针刺对慢性情绪应激焦虑大鼠血浆ANP、CORT和肾上腺ANP受体的影响

发布时间：2018-03-02 10:10

本文关键词： 针刺焦虑 ANP NPR-A CORT　出处：《辽宁中医杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:通过观察针刺对慢性情绪应激焦虑大鼠血浆心房利钠肽(atrial natriuretic peptide,ANP)、皮质酮(corticosterone,CORT)含量和肾上腺利钠肽A型受体(natriuretic peptide receptor type A,NPR-A)水平的影响,探讨针刺干预焦虑障碍模型的部分外周作用机理。方法:选取清洁级雄性SD大鼠30只,完全随机分为空白组、模型组、针刺组3组。运用不可预知的慢性情绪应激法建立焦虑动物模型,针刺组同时选取"内关""神门"予以治疗。以高架十字迷宫实验(elevated plus maze test,EPM)测试大鼠焦虑样行为,分别采用放射免疫法和ELISA法检测血浆ANP和CORT的含量,运用免疫组化法检测肾上腺皮质部NPR-A的水平。结果:与空白组对比,模型组的进入开臂次数(open-arms entries,OE)占入臂总次数的百分比值(OE%)显著下降(P0.01),血浆ANP、CORT值均明显升高(P0.05或P0.01);与模型组相比,针刺组OE%值有升高并接近空白组,血浆CORT显著下降(P0.01);3组肾上腺皮质NPR-A水平无显著性差异。结论:针刺可能通过降低血浆ANP和CORT的含量达到改善焦虑情绪的目的,而肾上腺皮质ANP受体的参与作用未显现。
[Abstract]:Objective: To observe the effect of acupuncture on chronic anxiety rats plasma atrial natriuretic peptide (atrial natriuretic, peptide, ANP), corticosterone (corticosterone, CORT) and the content of adrenal natriuretic peptide receptor type A (natriuretic peptide receptor type A, NPR-A) levels, and to explore the mechanism of intervention of anxiety disorder model part peripheral mechanism methods: 30 male SD rats were selected and randomly divided into blank group, model group, acupuncture group 3 groups. Using the method of chronic emotional stress unpredictable to establish animal models of anxiety, the acupuncture group and select the "Neiguan" and "Shenmen" treatment. With the elevated plus maze test (elevated plus maze test, EPM) test rats anxiety like behavior, the content of plasma ANP and CORT were detected by radioimmunoassay and ELISA method, using immunohistochemical method to detect adrenal NPR-A level. Results: compared with the control group of Ratio of open arm entries into the model group (open-arms entries, OE)% of the ratio of the total number of arm entries (OE%) decreased significantly (P0.01), plasma ANP, CORT values were significantly increased (P0.05 or P0.01); compared with the model group, the acupuncture group had elevated OE% value and close to the blank control group, plasma CORT was significantly decreased (P0.01); there was no significant difference in the adrenal cortex NPR-A levels in the 3 groups. Conclusion: acupuncture can decrease the plasma levels of ANP and CORT can improve the anxiety, but does not appear in the effects of the adrenal cortex ANP receptor.

【作者单位】：成都中医药大学针灸推拿学院;
【基金】：国家自然科学基金项目(81173322) 教育部博士点基金专项科研基金博导类课题(20135132110009)
【分类号】：R245

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