肾阳虚致高脂血症大鼠中FAS蛋白对甘油三酯的调控作用及机制研究

发布时间：2018-03-02 12:08

本文选题：LXRα　切入点：SREBP-c　出处：《中华中医药学刊》2017年05期 　论文类型：期刊论文

【摘要】：目的:通过肾阳虚制作高脂血症模型,观察右归丸对模型大鼠FAS蛋白及其上游LXRα等因子表达的影响,以探讨肾阳虚对模型大鼠甘油三酯(TG)的影响机制。方法:利用大剂量肌注氢化可的松制作肾阳虚高脂血症大鼠模型,给予右归丸进行干预,通过蛋白免疫印迹法(Western blot)检测实验大鼠肝组织FAS蛋白、LXRα和SREBP-1c表达,通过免疫组化检测实验大鼠肝组织FAS蛋白的表达。结果:大剂量肌注氢化可的松成功复制出了肾阳虚高脂血症的模型大鼠,与正常组比较,右归丸组和模型组肾阳虚高脂血症大鼠肝内LXRα、SREBP-1c和FAS蛋白表达增加(P0.05),与模型组比较,右归丸组大鼠肝内LXRα、SREBP-1c和FAS蛋白表达减少(P0.05);结论:肌注氢化可的松制造的肾阳虚高脂血症模型可能是上调了LXRα、SREBP-1c和FAS的蛋白的表达而影响了血液中TG的代谢,右归丸可能通过抑制LXRα、SREBP-1c和FAS蛋白的表达来降低模型大鼠TG的含量。
[Abstract]:Objective: to observe the effect of Yougui pill on the expression of FAS protein and LXR 伪 in the upper reaches of the model rats by making hyperlipidemia model with deficiency of kidney yang. To investigate the effect mechanism of kidney-yang deficiency on triglyceride triglyceride (TG) in model rats. Methods: the rat model of kidney yang deficiency hyperlipidemia was made by high dose intramuscular injection of hydrocortisone and treated with Yougui Pill. The expression of LXR 伪 and SREBP-1c in liver tissue of experimental rats was detected by Western blotting. The expression of FAS protein in liver tissue of experimental rats was detected by immunohistochemistry. Results: the model rats with kidney yang deficiency and hyperlipidemia were successfully duplicated by high dose intramuscular injection of hydrocortisone, which was compared with the normal group. The expression of LXR 伪 -SREBP-1c and FAS protein in liver of rats with kidney yang deficiency and hyperlipidemia were increased in Yougui pills group and model group, compared with model group. Conclusion: the hyperlipidemia model of kidney yang deficiency induced by hydrocortisone could up-regulate the expression of LXR 伪 -SREBP-1c and FAS and affect the metabolism of TG in blood. Yougui Pill may reduce the TG content of model rats by inhibiting the expression of LXR 伪 -SREBP-1c and FAS protein.
【作者单位】：湖北中医药大学;
【基金】：国家自然科学基金项目(81202615)
【分类号】：R259

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