不同强度按法对急性闭合性软组织损伤家兔的影响

发布时间：2018-03-03 07:31

本文选题：软组织损伤　切入点：五羟色胺　出处：《浙江中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的观察不同强度按法对急性闭合性软组织损伤家兔的五羟色胺(5-hydroxytryptamine,5-HT)、前列腺素E2(prostaglandin E2, PGE2)以及病理学上的影响。方法动物采用中国长耳兔,体重在2.5-3kg由浙江中医药大学动物实验中心提供,造模前先将中国长耳兔适应性饲养1周,造模成功的100只中国长耳兔随机平均分为对照组、实验A(250g)组、实验B(500g)组、实验C(1000g)组和实验D(2000g)组,每组20只。再根据处死时间(按压10min后、第2天、第四天及第八天)分为1、2、3、4四个亚组；即对照组1,实验组A1、B1、C1、D1,每组五只。所有动物在造模成功后及干预后处死前取耳缘静脉血4ml左右用于五羟色胺的检测。剪取处死兔受打击部位的中心且呈青紫色的肌肉组织,做苏木精-伊红染色用于病理观察以及前列腺素E2的检测。结果各实验组均可降低外周血中五羟色胺的含量,且各组间无统计学差异。各实验组均降低了前列腺素E2的含量,但A组与B组优于C组及D组。A与B两组间无统计学差异,C与D间也无统计学差异。各实验组均不能改善急性闭合性软组织损伤兔的病理学指标,且与对照组及各组之间无明显差异。结论不同强度按法对急性闭合性软组织损伤兔的五羟色胺及前列腺素E2都有调节作用,250g和500g压力组疗效更为显著。几种强度按法均不能改善急性闭合性软组织损伤兔病理学指标。
[Abstract]:Objective to observe the effects of different intensities on serotonin 5-hydroxytryptamine 5-HTG, prostaglandin E2prostaglandin E2 (PGE2) and pathology in rabbits with acute closed soft tissue injury. The weight of 2.5-3kg was provided by the Animal experiment Center of Zhejiang University of traditional Chinese Medicine. 100 Chinese long-eared rabbits were fed adaptively for one week before modeling. The 100 Chinese long-eared rabbits were randomly divided into two groups: control group, experimental group (250 g) and experimental group (B _ (500 g)). 20 rats in each group were divided into 4 subgroups according to the time of execution (after 10 minutes, 2 days, 4th days and 8 days). That is, the control group 1, the experimental group A1B1B1C1C1D1, each group of five. All animals after the success of the model and the intervention before the death of the auricular vein blood about 4 ml for the detection of serotonin. Cut to kill the rabbit hit the center of the muscle tissue and blue color, Hematoxylin-eosin staining was used for pathological observation and prostaglandin E2 detection. Results all experimental groups could reduce the content of serotonin in peripheral blood, and there was no statistical difference among the groups. The content of prostaglandin E 2 was decreased in each experimental group. But there was no statistical difference between group A and group B compared with group C and group D. there was no significant difference between group C and group D. all experimental groups could not improve the pathological indexes of rabbits with acute closed soft tissue injury. Conclusion the effect of different intensities on serotonin and prostaglandin E _ 2 in acute closed soft tissue injury rabbits is more significant than that in the pressure group of 250g and 500g. The pathological indexes of rabbits with acute closed soft tissue injury could not be improved by the method.
【学位授予单位】：浙江中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R244.1

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