针刺联合亚低温对脑缺血再灌注大鼠相关miRNA功能的影响

发布时间：2018-03-05 02:30

本文选题：针刺　切入点：亚低温　出处：《湖南中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:通过观察针刺联合亚低温对脑缺血再灌注损伤(CIRI)模型大鼠神经功能缺损评分、梗死面积比、Mi RNA的表达的影响,探讨针刺联合亚低温是否能通过调控CIRI大鼠的相关Mi RNA功能,产生脑保护效应。方法:将60只SD大鼠随机分为空白组、假手术组、模型组、针刺组、亚低温组、针刺联合亚低温组,参照Zea Longa线拴法并加以改良,复制大脑中动脉缺血模型(MCAO),针刺组、亚低温组、联合组做相应治疗,治疗72h后,用基因微阵列技术筛选差异表达mi RNA,对比其功能,并于对大鼠行神经功能缺损评分、TTC染色检测的梗死面积比进行比较。结果:1、各组大鼠神经功能缺损评分的比较:治疗前,与空白组及假手术组比较,针刺组、亚低温组、针刺联合亚低温组神经功能缺损评分均降低,差异具有统计学意义(P0.05或P0.01);治疗后,与空白组及假手术组比较,模型组、针刺组、亚低温组神经功能缺损评分较高,差异具有显著统计学意义(P0.01),与模型组比较,针刺组、亚低温组、针刺联合亚低温组均能降低神经功能缺损评分,差异具统计学意义(P0.05或P0.01),各治疗组之间相比,差异无统计学意义(P0.05)。2、各组大鼠梗死面积比的比较:与空白组及假手术组比较,模型组、针刺组、亚低温组及针刺联合亚低温组梗死面积比均明显增加,差异有统计学意义(P0.01);与模型组比较,针刺组、亚低温组及针刺联合亚低温组梗死面积比均减少,差异有统计学意义(P0.05或P0.01)。3、与空白组比较,模型组差异表达mi RNAs个数为4;其功用参与细胞增殖和细胞凋亡调控;与模型组相比:差异表达mi RNAs数,针刺组为16、亚低温组为7,针刺联合亚低温组为23;其功用都参与调节细胞增殖、基因表达、细胞凋亡、神经元分化、轴突延伸、炎性因子产生,且参与每项功用的mi RNAs数目均不同。结论:脑缺血再灌后有多种mi RNAs功用失调,针刺联合亚低温可以改善神经功能,保护脑组织,其作用机制可能与多方面、多环节调节相关mi RNAs的功用有关。
[Abstract]:Objective: to observe the effect of acupuncture combined with mild hypothermia on neurological impairment score and infarct area ratio of Mi RNA in rats with cerebral ischemia-reperfusion injury. To investigate whether acupuncture combined with mild hypothermia can produce brain protective effect by regulating the function of Mi RNA in CIRI rats. Methods: sixty SD rats were randomly divided into blank group, sham operation group, model group, acupuncture group and mild hypothermia group. Acupuncture combined with mild hypothermia group, referring to the method of Zea Longa thread tying and improving, the model of middle cerebral artery ischemia (MCAO), acupuncture group, mild hypothermia group, combined group were treated with corresponding therapy after 72 hours of treatment. The differentially expressed mi RNAs were screened by gene microarray technique, and their functions were compared, and the infarct area ratio detected by TTC staining was compared in rats. Results: 1, comparison of neurological deficit scores in each group: before treatment, Compared with the blank group and sham operation group, the scores of nerve function defect in acupuncture group, mild hypothermia group, acupuncture combined with hypothermia group were all decreased, the difference was statistically significant (P0.05 or P0.01), after treatment, compared with blank group and sham operation group, model group, acupuncture group, Compared with the model group, acupuncture group, mild hypothermia group and acupuncture combined with hypothermia group all decreased the neurological function defect score. The difference was statistically significant (P 0.05 or P 0.01). There was no significant difference between the treatment group and the treatment group. The ratio of infarct area in each group was: compared with the blank group and sham operation group, the model group, the acupuncture group, the control group, the control group, the acupuncture group, the control group, the model group, the acupuncture group, the control group, the model group and the acupuncture group. The infarct area ratio of mild hypothermia group and acupuncture combined with mild hypothermia group was significantly increased, the difference was statistically significant (P 0.01), compared with model group, the infarct area ratio of acupuncture group, mild hypothermia group and acupuncture combined with mild hypothermia group were all decreased. Compared with the blank group, the number of RNAs expressed in the model group was 4; its function was involved in the regulation of cell proliferation and apoptosis; compared with the model group, the number of the difference expressed mi RNAs was higher than that in the model group. 16 in acupuncture group, 7 in mild hypothermia group and 23 in acupuncture combined with mild hypothermia group. Their functions were involved in regulating cell proliferation, gene expression, apoptosis, neuronal differentiation, axon extension and inflammatory factor production. The number of mi RNAs involved in each function is different. Conclusion: there are many kinds of RNAs dysfunction after cerebral ischemia reperfusion. Acupuncture combined with mild hypothermia can improve nerve function and protect brain tissue. Multi-link regulation related to the function of the relevant mi RNAs.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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