支气管哮喘寒饮蕴肺证大鼠气道炎症机理及温阳化饮方作用机制研究

发布时间：2018-03-08 14:02

本文选题：1　切入点：25(OH)2D3　出处：《山东中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:探讨大鼠支气管哮喘寒饮蕴肺证气道炎症的机理,研究温阳化饮方作用机制。方法:采用卵蛋白致敏加冰箱冷冻、冰水饲养、温水游泳等方法,建立支气管哮喘寒饮蕴肺证大鼠模型,分别用1,25(OH)2D3、小青龙汤、温阳化饮方和六君子汤进行干预,观察相关检测指标。结果:造模较为成功,模型组大鼠的一般行为学表现与支气管哮喘寒饮蕴肺证的临床表现基本相符,微观指标的变化也证实造模较为成功;治疗组的四种药物对上述各项指标都有不同程度的改善,其中温阳化饮方疗效最佳;小青龙汤组和温阳化饮方组药物均对大鼠血液1,25(OH)2D3含量有明显促进作用,其中,温阳化饮方组作用最显著,而1,25(OH)2D3组和六君子汤组对大鼠血液1,25(OH)2D3含量促进作用不明显。结论:针对支气管哮喘寒饮蕴肺证,温阳化饮方确有较好疗效。同时发现温阳化饮方能提升支气管哮喘寒饮蕴肺证大鼠血液中1,25(OH)2D3的含量,提示温阳化饮方疗效机制可能是通过改善大鼠血液中1,25(OH)2D3的含量,进而改善气道炎症和气道重塑。说明温阳化饮方的温补肺阳,温化寒饮及改善肺通气的作用,与其调控体内1,25(OH)2D3的含量有关。
[Abstract]:Objective: to explore the mechanism of airway inflammation in rat bronchial asthma with cold drink retention of lung syndrome, and to study the mechanism of warming Yanghua decoction. Methods: egg protein sensitization and refrigerator freezing, ice water feeding, warm water swimming and so on were used. The rat model of asthmatic cold drink accumulation of lung syndrome was established. The rats were interfered with 1J 25 OHN 2D3, Xiao Azure Dragon decoction, Wen Yang Hua Yin Fang and Liujunzi decoction, respectively. Results: the model was made successfully. The general behavior of the rats in the model group was basically consistent with the clinical manifestation of cold drink accumulation of lung syndrome in bronchial asthma, and the changes of microscopic indexes also confirmed that the model was more successful, and the four drugs in the treatment group all improved the above indexes to some extent. Among them, Wenyanghua decoction had the best curative effect, both of Xiao#china_person0# decoction group and Wenyanghua decoction group had obvious promoting effect on the blood of rats, among which, the effect of Wenyanghua decoction group was the most significant. However, the effects of OHH 2D3 group and Liujunzi decoction group on the content of OHN 2D3 in blood of rats were not obvious. Conclusion: according to the syndrome of cold drink and lung accumulation in bronchial asthma, there is no obvious effect on promoting the content of OHO 2D3 in the blood of rats. At the same time, it was found that Wenyanghua decoction could elevate the content of OHH 2D3 in the blood of rats with cold drink accumulation syndrome of bronchial asthma, suggesting that the therapeutic mechanism of Wenyanghua decoction might be by improving the content of OH2D3 in the blood of rats. The effect of Wenyanghua decoction on warming the lung yang, warming the cold drink and improving the lung ventilation is related to the regulation of the content of OHH 2D3 in the body.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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