超声生物显微镜对温经通络法干预胶原诱导性关节炎大鼠滑膜增生的研究

发布时间：2018-03-13 16:05

  本文选题：超声生物显微镜　切入点：温经通络法　出处：《中华中医药学刊》2017年09期 　论文类型：期刊论文

【摘要】：目的:以超声生物显微镜(UBM)观察温经通络法干预胶原诱导性关节炎(CIA)大鼠滑膜增生情况。方法:40只SPF级雌性Wistar大鼠,随机分为正常组、模型组、对照组和中药组,每组10只。正常组常规饲养,其余各组复制胶原诱导性关节炎(CIA)大鼠模型后,分别以蒸馏水、雷公藤多苷片和加味芍甘附子汤干预4周,观察大鼠关节炎症评分(AI)和双足排水体积,超声生物显微镜观察治疗后各组大鼠跖趾关节病变情况,并与组织病理学结果进行对比。结果:造模后14~21 d,除正常组外各组AI值和双足排水体积均显著升高,给药后各组肿胀持续减轻,AI值降低。造模后42 d,中药组大鼠右足排水体积显著低于对照组(P0.05),同时期各组间AI和双足排水体积比较差异无统计学意义(P0.05);UBM平扫正常组可见骨皮质为连续的、致密强回声带,滑膜无显著增厚,厚度低于0.1 mm,其余3组关节间隙内均可见不同程度中等回声信号,其中模型组骨面毛糙,边缘欠整齐最明显,与病理学结果相符;滑膜病理评分与滑膜厚度呈正相关(r=0.637,P=0.002),与AI评分无显著相关性(r=0.192,P=0.344)。结论:UBM可用于检测RA动物模型早期病理改变,对疗效观察和预后评估具有较好的潜在价值。
[Abstract]:Objective: to observe the synovium proliferation of collagen-induced arthritis (CIA) rats by ultrasound biomicroscopy (UBMM). Methods: 40 SPF female Wistar rats were randomly divided into normal group, model group, control group and traditional Chinese medicine group. Ten rats in each group were fed with the normal control group, and the other groups were treated with distilled water, tripterygium wilfordii polyglycoside tablets and modified Paeoniao Gan Fuzi decoction for 4 weeks after the model of collagen-induced arthritis (CIA) was established in each group. The arthritis score of rats (AII) and the drainage volume of both feet were observed. The pathological changes of metatarsophalangeal joint were observed by ultrasound biomicroscopy. The results were compared with those of histopathology. Results: the AI value and biped drainage volume increased significantly in each group except the normal group at day 14 and 21 after modeling. The volume of draining of right foot in the Chinese medicine group was significantly lower than that of the control group at 42 days after administration of the drug, and there was no significant difference in AI and the volume of drainage between the two groups at the same time. We can see that the bone cortex is continuous. The thickness of synovium was less than 0.1 mm and the thickness of synovium was less than 0.1 mm. The other three groups showed moderate echo signal in the space of joint, in which the bone surface of model group was rough and the edge was not neat, which was consistent with the pathological results. There was a positive correlation between synovial pathological score and synovial thickness. There was no significant correlation between synovial pathological score and AI score. Conclusion: the early pathological changes of RA animal model can be detected by WUBM, which has a good potential value for curative effect observation and prognosis evaluation. [WT5 "HZ] [WT5" BZ] [WT5BZ] [WT5BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5BZ]
【作者单位】： 河南省中医院;北京中医药大学东方医院;北京中医药大学;
【基金】：国家自然科学基金项目(81273697)
【分类号】：R259
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本文编号：1607105