维药神香草提取物体外抗炎作用的谱效关系研究

发布时间：2018-03-15 04:12

本文选题：神香草　切入点：不同极性提取物　出处：《中国药房》2017年10期 　论文类型：期刊论文

【摘要】：目的:为阐明维药神香草抗炎作用的药效物质基础提供参考。方法:采用高效液相色谱法建立神香草8个不同极性提取物的指纹图谱;以巨噬细胞RAW264.7为研究对象,考察神香草不同极性提取物对细胞培养上清液中炎症因子一氧化氮(NO)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的抑制作用,比较其体外抗炎活性的差异性;采用灰色关联分析法分析指纹图谱共有峰的峰面积与抗炎活性的谱-效关系。结果:神香草8个不同极性提取物的指纹图谱具有明显的差异。体外抗炎结果提示,神香草30%乙醇提取物对体外炎症因子的抑制作用最强。对其建立的HPLC指纹图谱有共有峰14个,与体外炎症因子的抑制作用关系密切的5个共有峰分别为8、6、5、10、13号,其中8号峰为迷迭香酸。结论:所建立的指纹图谱及其与体外抗炎活性的谱效关系可为该药材的药效物质基础研究提供一定参考。
[Abstract]:Objective: to provide a reference for elucidating the substance basis of the anti-inflammatory effect of Herba Vulgaris. Methods: the fingerprint of 8 different polar extracts of Herba Shenxiangensis was established by high performance liquid chromatography (HPLC), and the macrophage RAW264.7 was used as the research object. To investigate the inhibitory effects of different polarity extracts of Shenxiang on inflammatory factors nitric oxide (no), tumor necrosis factor 伪 (TNF- 伪) and interleukin-6 (IL-6) in the supernatant of cell culture, and to compare their anti-inflammatory activities in vitro. The relationship between the peak area of the common peak and the anti-inflammatory activity was analyzed by grey relational analysis. Results: there were significant differences in the fingerprints of 8 different polar extracts of Herba Shenxianensis in vitro. Shenxiang 30% ethanol extract had the strongest inhibitory effect on inflammatory factors in vitro. There were 14 peaks in the HPLC fingerprint and 5 common peaks, which were closely related to the inhibitory effect of in vitro inflammatory factors. Conclusion: the established fingerprint and its relationship with the anti-inflammatory activity in vitro can provide some reference for the basic study of the pharmacodynamics of Rosemary acid.
【作者单位】：新疆维吾尔自治区药物研究所;中国医学科学院北京协和医学院药物研究所/天然药物活性物质与功能国家重点实验室;
【基金】：国家自然科学基金资助项目(No.81560644) 新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(No.KY2014062) 新疆维吾尔自治区中医民族医药科技人才培养项目(No.2016-03-03)
【分类号】：R29

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