电针不同神经节段穴位对OT基因敲除小鼠胃排空的影响

发布时间：2018-03-17 13:52

本文选题：OT基因敲除小鼠　切入点：电针　出处：《中国针灸》2017年07期 　论文类型：期刊论文

【摘要】：目的:观察电针不同神经节段穴位对催产素(oxytocin,OT)基因敲除小鼠胃排空的影响,探讨室旁核OT在电针不同神经节段穴位调节胃功能活动中的作用。方法:8~9周龄OT基因敲除型及野生型小鼠两个亚组各20只,雌雄各半,每个亚组分别随机分为空白对照组、针刺足三里组、针刺内关组和针刺胃俞组,每组5只。空白对照组不进行针刺处理,针刺足三里组、针刺内关组和针刺胃俞组分别电针单侧"足三里""内关""胃俞"穴15 min,随后用同位素锝-亚锡喷替酸(~(99m)Tc-DTPA)标记的牛奶0.1 m L灌胃(放射性活度为3 mCi/m L),灌胃完成后即刻用单光子发射型计算机断层扫描仪(SPECT)行胃排空检测50 min,用专用软件分析采集的原始数据,得到全胃半排时间(GET 1/2)、50 min末胃残留率、胃排空曲线。结果:野生型小鼠组:与空白对照组相比,电针"足三里"和"内关"后GET 1/2、50 min末胃残留率明显减少,可加快野生型小鼠胃排空(P0.01,P0.05),电针"胃俞"后GET 1/2、50 min末胃残留率明显增加,可抑制野生型小鼠胃排空(P0.01,P0.05);OT基因敲除型小鼠组:与空白对照组相比,电针"足三里"和"内关"对OT敲除型小鼠胃排空无显著影响(均P0.05),电针"胃俞"后GET 1/2、50 min末胃残留率明显增加,可抑制OT敲除型小鼠胃排空(均P0.01);胃排空曲线:OT敲除型小鼠的胃排空明显快于野生型小鼠。结论:电针不同神经节段穴位对小鼠的胃排空影响程度不同,OT基因敲除可加速小鼠胃排空,且影响部分穴位的针刺效应,提示作为自主神经通路始动器的室旁核OT神经元,可能以神经及内分泌的形式参与电针不同神经节段穴位对胃功能的调节,抑制胃排空。
[Abstract]:Objective: to observe the effect of electroacupuncture on gastric emptying of oxytocin OTO gene knockout mice. To investigate the role of OT in the regulation of gastric function at acupoints of different segments of nerve segments in electroacupuncture. Methods two subgroups (20 female and half male) were randomly divided into control group (n = 20) and wild-type mice (n = 20) at the age of 9 weeks, and each subgroup was randomly divided into two subgroups: control group (n = 20) and control group (n = 20). Five rats in each group were treated with acupuncture in Zusanli group, Neiguan group and Weishu group respectively. The blank control group was not treated with acupuncture, and the Zusanli group was treated with acupuncture. Acupuncture in Neiguan group and Weishu group in acupuncture group were electroacupuncture at "Zusanli" and "Neiguan" and "Weishu" for 15 minutes respectively, and then 0.1 mL of milk labeled with Tc-DTPA-labeled with Tc-DTPA-labeled with Tc-DTPA-labeled with Tc-DTPA-labeled milk (radioactivity of 3 mCi/m / L) was perfused (3 mCi/m / L) after gastric perfusion was completed. Single photon emission computed tomography (SPECT) was used to detect gastric emptying for 50 min, and the original data was analyzed with special software. The gastric remnant rate and gastric emptying curve at the end of 50 min were obtained. Results: compared with the control group, the gastric remnant rate of GET 1 / 2 ~ 50 min after electroacupuncture in Zusanli and Neiguan groups was significantly decreased. It could accelerate the gastric emptying of wild type mice (P0.01P0.05N), increase the gastric residual rate of GET 1 / 2 / 2 ~ (50) min after electroacupuncture of "Weishu", and inhibit the gastric emptying of wild type mice by P0.01P0.05OT-gene knockout mice: compared with the blank control group, there was no significant difference in gastric emptying rate between the control group and the control group. Electroacupuncture "Zusanli" and "Neiguan" had no significant effect on gastric emptying in OT knockout mice (all P 0.05), and the gastric residual rate at the end of GET 1 / 2 ~ 50 min after electroacupuncture "Weishu" increased significantly. It can inhibit gastric emptying in OT knockout mice (all P 0.01); gastric emptying of mice with gastric emptying curve of 1: OT knockout is faster than that of wild type mice. Conclusion: electroacupuncture at different nerve segment points has different effects on gastric emptying in mice with different OT gene knockout. In addition to accelerating gastric emptying in mice, It is suggested that the OT neurons in paraventricular nucleus, which are the initiator of autonomic nerve pathway, may participate in the regulation of gastric function by electroacupuncture at different nerve segments in the form of nerve and endocrine, and inhibit gastric emptying.
【作者单位】：湖南中医药大学针灸推拿学院;
【基金】：国家自然科学基金资助项目:81273677
【分类号】：R245

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