自然衰老法与卵巢去势法建立围绝经期小鼠模型的比较研究

发布时间：2018-03-17 16:02

本文选题：自然衰老　切入点：卵巢去势　出处：《中华中医药学刊》2017年03期 　论文类型：期刊论文

【摘要】：目的:比较自然衰老法与卵巢去势法建立的ICR小鼠围绝经期模型,并对模型进行评价。方法:通过观察阴道脱落细胞,比较自然衰老组及卵巢去势组小鼠的动情周期情况;观察小鼠悬尾活动,比较自然衰老组及卵巢去势组小鼠的情绪状态;测定血清性激素,比较血清卵泡刺激素(FSH)、促黄体激素(LH)、雌二醇(E2)的含量;测定小鼠下丘脑神经肽,比较下丘脑Kiss-1及其受体GPR54 mRNA的表达。结果:自然衰老组及卵巢去势组小鼠动情周期均紊乱,动情频率均减少;两组小鼠悬尾活动比均显著提高;两组小鼠血清的FSH、LH水平显著上升,E2水平明显降低;两组小鼠的下丘脑Kiss-1及受体GPR54 mRNA的表达显著升高。相比卵巢去势小鼠,自然衰老小鼠动情周期相对延长,动情频率明显减少,血清的FSH、LH水平上升更显著,E2水平降低更显著,Ki SS-1/GPR54 mRNA的表达升高更明显。结论:自然衰老法与卵巢去势法均能建立小鼠围绝经期模型,而自然衰老法优于卵巢去势法。
[Abstract]:Objective: to compare the peri-menopausal model of ICR mice established by natural aging method and ovariectomized method, and to evaluate the model. Methods: by observing vaginal exfoliated cells, the estrous cycle of natural aging group and ovariectomized group was compared. To observe the activity of tail suspension in mice, to compare the emotional state of mice in natural aging group and ovariectomized group, to measure serum sex hormone, to compare the contents of serum follicle stimulating hormone FSHN, luteinizing hormone LHN, estradiol E _ 2, and to determine the neuropeptide of hypothalamus of mice. Results: the oestrous cycle of mice in natural aging group and ovariectomized group was disorder, and the frequency of estrus was decreased. The levels of Kiss-1 and GPR54 mRNA in hypothalamus of the two groups increased significantly. Compared with ovariectomized mice, the oestrous cycle and the frequency of estrus decreased significantly in the naturally aging mice. The serum level of FSH LH increased significantly and the level of E2 decreased significantly. The expression of Ki SS-1/GPR54 mRNA increased more obviously. Conclusion: both natural senescence and ovariectomization can establish the model of mouse peri-menopausal period, and the natural aging method is better than the ovariectomized method.
【作者单位】：杭州市中医院;湖州市中心医院;杭州市萧山区第一人民医院;
【基金】：全国名老中医药专家傅萍传承工作室建设项目([2014]20号) 浙江省科技厅项目(2013C33211) 浙江省中医药管理局项目(2013ZQ025) 浙江省卫生厅项目(2013KYA168) 杭州市科技局项目(20140733Q31) 浙江省名老中医专家傅萍传承工作室研究项目(GZS2012023)
【分类号】：R271.116;R-332

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