不同频率电针对阿尔茨海默病大鼠学习记忆能力效应及部分作用机制探讨

发布时间：2018-03-17 20:02

本文选题：阿尔茨海默症　切入点：电针　出处：《中国针灸》2017年06期 　论文类型：期刊论文

【摘要】：目的:观察不同频率电针"百会"与"肾俞"穴对阿尔茨海默病(AD)模型大鼠学习记忆能力和海马组织糖原合酶激酶-3β(GSK-3β)、生长相关蛋白-43(GAP-43)的影响,探讨不同频率电针防治AD的作用机制。方法:将112只Wistar雄性大鼠按随机数字表法分为正常组、假手术组、模型组、针刺组、2 Hz电针组、30 Hz电针组、50 Hz电针组,每组16只。正常组实验室常规饲养,不进行任何处理;假手术组于双侧海马齿状回注射0.9%Na Cl溶液;其他组采用双侧海马齿状回注射β-淀粉样蛋白1-42(Aβ1-42)制备AD模型。造模成功15 d后,模型组、假手术组不进行任何处理,2 Hz、30 Hz、50 Hz电针组大鼠选"百会""肾俞"穴进行相应频率电针治疗,每日1次,7次为一疗程,疗程间休息1 d,共治疗2个疗程。针刺组选穴与电针组相同,但仅针刺不接电针。治疗结束后立即进行水迷宫检测,记录大鼠逃避潜伏期、首次跨越平台时间、跨越平台次数,并分别采用免疫组化法和Western blot检测大鼠海马组织GSK-3β、GAP-43的表达。结果:(1)水迷宫测试示,与正常组比较,模型组大鼠逃避潜伏期、首次跨越平台时间延长(均P0.01),跨越平台次数明显减少(P0.01)。针刺组、3组电针组与模型组比较,逃避潜伏期、首次跨越平台的时间缩短(均P0.01),跨越平台次数明显增加(P0.01)。50 Hz电针组与针刺组、2 Hz、30 Hz电针组比较,逃避潜伏期缩短(P0.01,P0.05)、首次跨越平台时间缩短(均P0.01),跨越平台次数明显增加(P0.01,P0.05)。(2)与正常组比较,模型组GSK-3β、GAP-43表达明显增多(均P0.01)。与模型组比较,针刺组和3组电针组GSK-3β表达明显减少(均P0.01),GAP-43表达明显增多(均P0.01)。与针刺组、2 Hz、30 Hz电针组比较,50 Hz电针组GSK-3β表达明显减少,GAP-43表达增加(均P0.01)。结论:电针可能通过下调GSK-3β、上调GAP-43表达,从而促进突触损伤修复,改善AD大鼠的学习记忆能力。50 Hz电针治疗效果优于30 Hz、2 Hz电针组及针刺组。
[Abstract]:Objective: to observe the effects of electroacupuncture of Baihui and Shenshu on learning and memory ability and glycogen synthase kinase-3 尾 -GSK-3 尾 -43 GAP-43 in hippocampus of rats with Alzheimer's disease (AD). Methods: 112 male Wistar rats were randomly divided into normal group, sham operation group, model group and acupuncture group. 16 rats in each group were fed in the normal laboratory without any treatment, and 0.9 NaCl solution was injected into the dentate gyrus of both sides in the sham operation group. The AD model was established by injecting 尾 -amyloid protein 1-42A 尾 1-42 into the dentate gyrus of the other groups. After 15 days of successful modeling, the model group was treated with 尾 -amyloid protein 1-42A 尾 1-42. Rats in the sham operation group were treated with "Baihui" and "Shenshu" at the corresponding frequency, 7 times a day as a course of treatment. The acupuncture group selected points the same as the electroacupuncture group, but only the acupuncture group did not receive electroacupuncture. After the treatment, the water maze test was carried out immediately, the escape latency of rats was recorded, the first time of crossing the platform, the times of crossing the platform, the time of crossing the platform, the time of crossing the platform for the first time, and the number of times of crossing the platform. Immunohistochemical method and Western blot were used to detect the expression of GSK-3 尾 -GAP-43 in hippocampus of rats. Results compared with the normal group, the escape latency of the model group was higher than that of the normal group. The time of crossing the platform was prolonged for the first time (all P0.01A, the times of crossing the platform were significantly decreased P0.01.The escape latency of EA group and model group was compared with that of model group. The time of crossing the platform for the first time was shortened (all P 0.01), and the times of crossing the platform were significantly increased compared with the acupuncture group and the acupuncture group. Compared with the normal group, the expression of GSK-3 尾 -GAP-43 in the model group was significantly higher than that in the normal group (P 0.01). The expression of GSK-3 尾 in acupuncture group and electroacupuncture group was significantly decreased (P 0.01) and the expression of GAP-43 was significantly increased (all P 0.01). Compared with acupuncture group, the expression of GSK-3 尾 in 50 Hz electroacupuncture group decreased significantly (all P 0.01). Conclusion: electroacupuncture may increase the expression of GSK-3 尾 (P 0.01). Regulation of GSK-3 尾, up-regulation of GAP-43 expression, The results showed that the effect of electroacupuncture on learning and memory ability of AD rats was better than that of 30 Hz / 2 Hz electroacupuncture group and 30 Hz / 2 Hz electroacupuncture group, so as to promote the repair of synaptic injury and improve the learning and memory ability of AD rats.
【作者单位】：湖北中医药大学;湖北中医药大学针灸骨伤学院;
【基金】：国家自然科学基金资助项目:81373741
【分类号】：R245

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