藏药榜嘎质量标准研究

发布时间：2018-03-18 14:35

本文选题：榜嘎　切入点：生物碱　出处：《成都中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：榜嘎(Bangga)为毛茛科(Ranunculaceae)乌头属(Aconitum)多年生草本植物,是习用藏药材之一,味苦性凉,有小毒,民间及临床的应用均取其抗炎、清热解毒、抗肿瘤等药理作用,多种藏药复方中均含榜嘎。文献分析发现榜嘎药材的主要毒性及活性成分为生物碱类,榜嘎药材的广泛使用与其所含生物碱成分的药理作用密切相关,但其质量标准极完善,缺乏科学及系统性,限制临床用药的安全性。该课题开展了榜嘎药材生物碱成分的提取、分离、纯化及鉴定研究,从中得到3个化合物,分别鉴定为：异叶乌头碱、大麦芽碱、塔拉乌头胺,纯度符合对照品要求,采用HPLC及TLC分析发现大麦芽碱为榜嘎药材共有生物碱成分,且具有显著的药理活性,故将其定为榜嘎药材质量评价指标。采用LC-MS对毒性双酯型生物碱乌头碱、新乌头碱、次乌头碱进行了定性分析,研究发现,榜嘎药材中不含乌头碱、次乌头碱、新乌头碱成分,与文献报道一致。故本研究未对毒性成分乌头碱、新乌头碱及次乌头碱进行限量测定。在榜嘎药材质量标准的研究中,以大麦芽碱为检测指标。采用薄层鉴别法,建立了以大麦芽碱为活性指标成分的薄层鉴别方法；采用高效液相色谱法法,建立了以大麦芽碱为检测指标的含量测定方法,并测定了多批药材中大麦芽碱含量,规定其含量不得低于0.009%；照《中国药典》2015年版(四部)通则方法测定榜嘎药材水分、灰分、浸出物,规定了其水分不大于9.5%,总灰分不大于15.5%,酸不溶性灰分不大于2.5%,浸出物不得少于23.4%。在对榜嘎药材的生物碱成分的活性研究中,采用烟熏法造急性支气管炎模型,观察了大麦芽碱、异叶乌头碱、塔拉乌头胺对气管炎的防治作用。实验结果表明,3种生物碱均有一定的抗炎活性,但大麦芽碱的药理活性较强,为质量标准的建立提供研究支撑。本课题建立了榜嘎药材的质量标准,完善了性状、鉴别、检查、含量测定等检测项目,并对榜嘎特征性生物碱成分的药理活性进行了初步研究,为榜嘎药材的鉴定、资源的合理利用提供了研究支撑。
[Abstract]:Banggaa (Ranunculaceae) is a perennial herb of the genus Ranunculaceae). It is one of the traditional Tibetan medicinal herbs. It has a cool taste and has a small poison. The folk and clinical applications take its pharmacological effects of anti-inflammatory, heat-clearing and detoxifying, anti-tumor, and so on. The literature analysis showed that the main toxic and active components were alkaloids. The extensive use of Bongga was closely related to the pharmacological action of its alkaloids, but its quality standard was perfect. Lack of scientific and systematic methods to limit the safety of clinical drug use. In this paper, the extraction, isolation, purification and identification of alkaloids from Pangha were carried out, from which three compounds were obtained and identified as iso-aconitine and malt alkaloid, respectively. The purity of taraconitine met the requirement of reference substance. HPLC and TLC analysis showed that malt alkaloid was a common alkaloid component and had remarkable pharmacological activity. Therefore, it was determined as the quality evaluation index of Bongjia medicinal materials. The qualitative analysis of aconitine, new aconitine and subaconitine was carried out by LC-MS. The results showed that there were no aconitine, subaconitine and new aconitine in Bongga medicinal materials. Therefore, no limited determination of aconitine, new aconitine and subaconitine was carried out in this study. A TLC method for the identification of malt base was established, and a method for the determination of malt base was established by HPLC, and the content of malt base in many batches of medicinal materials was determined. It is stipulated that its content shall not be less than 0.009; according to the general rules of the 2015 edition (four parts) of the Chinese Pharmacopoeia, the water, ash, and extracts of the medicinal materials of Bongga shall be determined, Its water content is not greater than 9.5, total ash content is not greater than 15.5am, acid insoluble ash content is not more than 2.5, and extract is not less than 23.40.In the study of the activity of alkaloid components of Bongga medicinal herbs, the model of acute bronchitis was established by smoking method, and the malt base was observed. The experimental results showed that all three alkaloids had some anti-inflammatory activities, but the pharmacological activities of malt alkaloids were stronger. To provide research support for the establishment of quality standard. This subject has established the quality standard of Bongga medicinal materials, and has perfected the items of character, identification, inspection, content determination and so on. The pharmacological activities of the characteristic alkaloids were preliminarily studied, which provided the research support for the identification of the medicinal materials and the rational utilization of the resources.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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