壮医复方莲素饮对围绝经期模型大鼠骨代谢相关因子影响的实验研究

发布时间：2018-03-18 20:11

本文选题：围绝经期综合征　切入点：壮医复方莲素饮　出处：《广西中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察壮医复方莲素饮对围绝经期模型大鼠骨代谢相关因子的影响,探讨壮医复方莲素饮对围绝经期综合征模型大鼠骨代谢的作用机理。方法:选择成年雌性SD大鼠,腹腔注射4-VCD芝麻油溶剂造模15天,4-VCD剂量为80 mg/kg。选择造模成功大鼠50只,随机分为5组,每组10只,分别为复方莲素饮高、中、低剂量组、模型组、补佳乐组,另选未造模的10只作为正常对照组。对照组、模型组给予蒸馏水灌胃,复方莲素饮高、中、低剂量组分别按5.76g/kg、2.88g/kg、1.44g/kg剂量给予灌胃,补佳乐组按0.09mg/kg给予灌胃,各组连续灌胃给药21天。给药结束后,取样本,测定血清中E2、LH、FSH,降钙素基因相关肽(CGRP)、血管活性肠肽(VIP)的含量;子宫和卵巢组织中基质Gla蛋白(MGP)的表达。结果:壮医复方莲素饮能显著提升围绝经期模型大鼠血清中E2水平,与模型组相比,复方莲素饮低、中剂量组的E2水平升高,差异具有统计学意义(P0.05),补佳乐组与复方莲素饮高剂量组的E2水平明显升高,差异均具有显著统计学意义(P0.01);并降低血清中FSH、LH水平,与模型组相比,复方莲素饮低、中剂量组的FSH、LH水平下降,差异具有统计学意义(P0.05),补佳乐组与复方莲素饮高剂量组的FSH、LH水平明显下降,差异均具有显著统计学意义(P0.01);降低血清中CGRP、VIP水平,与模型组相比,复方莲素饮中剂量组的CGRP、VIP水平下降,差异具有统计学意义(P0.05),补佳乐组与复方莲素饮高剂量组的CGRP、VIP水平明显下降,差异均具有显著统计学意义(P0.01);显著提升子宫、卵巢组织中MGP的表达,与模型组相比,复方莲素饮中剂量组的MGP基因表达水平升高,差异具有统计学意义(P0.05),补佳乐组与复方莲素饮高剂量组的MGP基因表达水平明显升高,差异均具有显著统计学意义(P0.01)。结论:壮医复方莲素饮通过提升E2水平,降低LH、FSH水平,调控生殖轴,平衡机体紊乱的性激素;下调CGRP、VIP表达水平,改善骨代谢和血管舒缩功能,缓解因围绝经期雌激素水平下降引起的骨流失加速和骨合成能力下降等一系列骨代谢相关情况,以及血管舒张过度引起的机体潮热汗出症状;上调MGP表达水平,促进骨生成抑制骨破坏,改善异常骨代谢。综上,本方能够改善围绝经期综合征因激素神经内分泌紊乱而出现的潮热、骨代谢异常等情况,起到调理内分泌、调节血管舒缩,改善骨代谢的作用。
[Abstract]:Objective: to observe the effect of Lianxin decoction on bone metabolism related factors in peri-menopausal rats, and to explore the mechanism of bone metabolism in rats with perimenopausal syndrome. Methods: adult female SD rats were selected. The dose of 4-VCD was 80 mg / kg / kg for 15 days by intraperitoneal injection of 4-VCD sesame oil solvent. Fifty successful rats were randomly divided into 5 groups, 10 rats in each group, respectively: compound Lianyin high, middle and low dosage groups, model group and Bujiale group. Another 10 rats were selected as normal control group. The model group was given distilled water by gastric perfusion. The group of high, middle and low dosage of compound Liansu drink was given intragastric perfusion at the dose of 5.76 g / kg ~ 2.88 g / kg ~ (1.44) g / kg, and Bujiale group was given a dose of 0.09 mg / kg 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1), respectively. Each group was given orally for 21 days. After the administration, samples were taken to determine the contents of serum E _ 2 LHH _ (FSH), calcitonin gene-related peptide (CGRPN) and vasoactive intestinal peptide (VIP). The expression of matrix Gla protein in uterus and ovary. Results: the serum E _ 2 level of the model rats was significantly increased by Liansu decoction, compared with the model group, and the level of E _ 2 in the middle dose group was higher than that in the model group. The difference was statistically significant (P 0.05), the level of E _ 2 in the Bujiale group and the high dose group of compound Liansu decoction were significantly increased, the difference was statistically significant (P < 0.01), and the level of FSHN LH in serum was decreased, compared with the model group, the level of compound Lianyin drink was lower than that in the model group. The level of FSHP LH in the middle dose group decreased significantly (P 0.05), and the level of FSHP LH in the Bujiale group and the high dose group of Fufang Liansu Yin decreased significantly (P 0.01), and the level of CGRP VIP in the serum decreased, compared with the model group, the level of FSHP LH in the middle dose group was significantly lower than that in the model group. The level of CGRPU VIP in the middle dose group of Fufang Liansu decoction decreased, the difference was statistically significant (P 0.05), and the level of VIP in the Bujiale group and the high dose group of Fufang Liansuyin decoction decreased significantly, and the difference was statistically significant (P 0.01). Compared with the model group, the expression level of MGP gene in the middle dose group of Fufang Liansu decoction was higher than that in the model group, and the difference was statistically significant (P 0.05). The expression level of MGP gene in the Bujiale group and the high dose group of Fufang Liansuyin group was significantly higher than that in the model group. Conclusion: Liansu decoction can improve the level of E2, decrease the level of LHH FSH, regulate the reproductive axis, balance the sex hormone of body disorder, down-regulate the expression of CGRPnP VIP, improve bone metabolism and vasomotor function. To relieve a series of bone metabolism related conditions, such as bone loss acceleration and bone synthesis ability decline caused by the decline of estrogen level in perimenopausal period, as well as the symptoms of hot sweating caused by excessive vasodilation, and to upregulate the expression of MGP. To promote bone formation, inhibit bone destruction and improve abnormal bone metabolism. In summary, the prescription can improve the hot flashes and abnormal bone metabolism in peri-menopausal syndrome due to hormonal neuroendocrine disorders, and regulate endocrine, vasomotor and contraction, etc. The effect of improving bone metabolism.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29;R-332

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