瑶药岗松抗佐剂性大鼠关节炎的实验研究

发布时间：2018-03-19 14:57

本文选题：瑶药　切入点：岗松　出处：《中国民族民间医药》2017年05期 　论文类型：期刊论文

【摘要】：目的:观察瑶药岗松根醇提物(BFTE)对大鼠佐剂性关节炎(AA)的药理作用。方法:42只大鼠随机分为正常组、模型组、雷公藤多苷组及BFTE高、中、低剂量组,完全弗氏佐剂诱导AA后,于第12天开始连续灌胃给药16d;同一时间点测定大鼠足趾肿胀度、关节炎评分、免疫器官指数;第29天处死大鼠,酶联免疫法(ELISA)测定大鼠血清中TNF-α、IL-6、IL-1β的含量,光学显微镜观察大鼠踝关节的病理变化。结果:与模型组相比,BFTE能够显著抑制AA大鼠的原发性和继发性足跖肿胀度,降低关节炎评分、免疫器官系数,改善大鼠踝关节病理状态,下调AA大鼠血清中炎症因子TNF-α、IL-6和IL-1β的水平。结论:BFTE对AA大鼠具有良好的治疗作用,其抗炎免疫的机理可能与减少TNF-α、IL-6和IL-1β的分泌有关。
[Abstract]:Objective: to observe the pharmacological effect of the alcohol extract of yaoyaoyagang pine root on adjuvant arthritis (AAA) in rats. Methods: 42 rats were randomly divided into normal group, model group, tripterygium wilfordii polyglycoside group and BFTE high, middle and low dose groups, after complete Freund adjuvant induced AA. At the same time, the swelling degree of toes, arthritis score and immune organ index of rats were measured at the same time point. The rats were killed on the 29th day, and the content of TNF- 伪 IL-6 IL-6IL-1 尾 in serum was determined by Elisa. Results: compared with the model group, BFTE could significantly inhibit the primary and secondary plantar swelling, reduce the arthritis score and immune organ coefficient in AA rats. The levels of TNF- 伪 IL-6 and IL-1 尾 in the serum of AA rats were improved and the levels of TNF- 伪 -IL-6 and IL-1 尾 were down-regulated. Conclusion: BFTE has a good therapeutic effect on AA rats, and its mechanism of anti-inflammatory immunity may be related to the reduction of TNF- 伪 IL-6 and IL-1 尾 secretion.
【作者单位】：郑州大学药学院;河南省中医院;
【基金】：国家自然科学基金(NO.:81303162) 中国博士后科学基金(NO.:2015T80784、2014M552017) 河南省博士后科研资助(NO.:2013010)
【分类号】：R29

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