沿视觉传导通路电针疗法对前部缺血性视神经病变兔细胞凋亡干预的研究

发布时间：2018-03-21 15:04

本文选题：前部缺血性视神经病变　切入点：电针　出处：《中国针灸》2017年08期 　论文类型：期刊论文

【摘要】：目的:从形态学及细胞凋亡的角度探讨沿视觉传导通路电针疗法对缺血性视神经病变(anterior ischemic optic neuropathy,AION)兔模型视神经组织的保护作用。方法:采用激光方法制作前部缺血性视神经病变新西兰大耳白兔模型眼(右眼)48只,随机分为模型组、针刺组、针刺加电针组,每组16只,并随机选取16只左眼构成空白组。造模完成后次日针刺组与针刺加电针组穴取右侧"攒竹""鱼腰"和"窍明穴"分别进行针刺与针刺加电针干预,每天1次,每次30 min,连续3 d。模型组和空白组不予干预。通过HE染色观察各组兔视神经组织形态结构和视网膜内层厚度,免疫组化法观察视网膜中凋亡因子Bcl-2、Bax的表达,酶联免疫吸附反应法(ELISA)检测视网膜组织中肿瘤坏死因子α(TNF-α)浓度。结果:模型组神经节细胞层出现增生现象,排列紊乱,视网膜神经节细胞排列疏松,出现较长片段的视网膜神经节细胞缺失,内核层明显变薄,细胞数目减少;针刺组和针刺加电针组结构损伤程度明显减轻。视网膜内层厚度:模型组较空白组减小(P0.05),针刺加电针组高于针刺组及模型组(均P0.05),与正常组比较差异无统计学意义(P0.05)。Bcl-2计数:模型组较空白组低(P0.05),针刺加电针组较针刺组及模型组均高(均P0.05),与空白组比较差异无统计学意义(P0.05)。Bax计数:模型组较空白组高(P0.05),针刺加电针组较针刺组、模型组均低(均P0.05),且与空白组相当(P0.05)。Bcl-2/Bax:模型组低于空白组(P0.05),针刺加电针组均高于针刺组、模型组(均P0.05),且与空白组相当(P0.05)。TNF-α:模型组高于空白组(P0.05),其余各组间与模型组比较差异无统计学意义(均P0.05)。结论:沿视觉传导通路电针疗法对视神经组织结构具有一定的保护作用,可使Bcl-2表达增强,Bax表达减弱,从而抑制神经节细胞凋亡。
[Abstract]:Objective: to investigate the protective effect of electroacupuncture (EA) along visual conduction pathway on optic nerve tissue in rabbit model of ischemic ischemic optic neuropathy-AION from the angle of morphology and apoptosis. Methods: anterior ischemia was made by laser method. Model eyes of New Zealand large ear white rabbits with optic neuropathy (48 eyes of right eye). They were randomly divided into model group, acupuncture group, acupuncture plus electroacupuncture group with 16 rats in each group. 16 left eyes were randomly selected to form a blank group. The acupuncture group and the acupuncture plus electroacupuncture group took the right side of "Zanzhu" "Yu waist" and "Qiaoming acupoint" respectively for acupuncture and acupuncture with electroacupuncture once a day after the completion of the model. Each time for 30 minutes, the model group and the blank group were not interfered. The morphological structure of optic nerve and the thickness of the inner retinal layer were observed by HE staining, and the expression of apoptosis factor Bcl-2P Bax in the retina was observed by immunohistochemical method. The concentration of TNF- 伪 in retinal tissue was detected by Elisa. Results: in the model group, the ganglion cell layer was proliferated, disordered, and the retinal ganglion cells were loosely arranged. Long segments of retinal ganglion cells were absent, the nuclear layer became thinner and the number of cells decreased. The thickness of the inner layer of retina in the model group was lower than that in the blank group, and that in the acupuncture plus electroacupuncture group was higher than that in the acupuncture group and the model group (all P 0.05). There was no significant difference between the model group and the normal group. The count of P0.05U 路Bcl-2 in the model group was lower than that in the blank group, and that in the acupuncture plus electroacupuncture group was higher than that in the acupuncture group and the model group (P 0.05). There was no significant difference between the model group and the blank group in the count of P0.05U. Bax: the model group was higher than the blank group, and the acupuncture plus electroacupuncture group was higher than the acupuncture group. The model group was lower than that of the blank group (P0.05%, P0.05%. Bcl-2% Bax.The model group was lower than the blank group (P0.05%), and the acupuncture plus electroacupuncture group was higher than the acupuncture group, and the model group was lower than the blank group (P 0.05), and the electroacupuncture group was higher than that of the control group. The model group (all P0.05) and the same as the blank group (P0.05N. TNF- 伪): the model group was higher than the blank group (P0.05), and the other groups had no significant difference compared with the model group (all P0.050.Conclusion: electroacupuncture along the visual conduction pathway has a certain protective effect on the optic nerve tissue structure. The expression of Bcl-2 increased and the expression of Bax decreased, which inhibited the apoptosis of ganglion cells.
【作者单位】：中国中医科学院眼科医院;北京市第一中西医结合医院;北部战区空军参谋部门诊部;北京中医药大学东直门医院;
【基金】：北京市科技新星项目:z141107001814050
【分类号】：R245

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