地格达-4对幽门结扎性肝损伤大鼠PXR、CAR表达的影响

发布时间：2018-03-25 00:38

本文选题：地格达-　切入点：幽门结扎性肝损伤　出处：《中华中医药杂志》2017年12期

【摘要】：目的:研究地格达-4能否通过孕烷X受体(PXR)、组成型雄甾烷受体(CAR)表达变化治疗幽门结扎性肝损伤。方法:取50只大鼠,分为5组,即正常组、模型组、地格达-4的3个剂量(1.86、3.71、7.43g/kg)组,通过幽门结扎法建立大鼠肝损伤动物模型,灌胃给药共15d,采用实时荧光定量链式聚合酶反应(RTPCR)、蛋白印迹(Western blot)法检测大鼠肝脏组织中PXR、CAR表达变化及测定血清和肝组织中肝损伤敏感性指标。结果:与正常组比较,模型组血清AST、ALT、BILD2、BILT3、TBA显著增加(P0.01,P0.05),肝组织GSH-PX显著降低(P0.01);腹腔观察,腹膜具有明显的黄色、腹腔积水、黄尿、肉眼观察肝脏淤血;形态学观察,肝细胞有明显的脂肪变、水肿、边膜外肝细胞大量坏死、肝细胞具有一定程度的凋亡;与模型组比较,各给药组对AST、ALT、BILD2、BILT3、TBA显著降低(P0.01,P0.05),GSH-PX显著增高(P0.01);腹腔及肝组织形态具有一定好转;RT-PCR结果,地格达-4低、中、高组对核受体CAR上调至9 739、3 066、1 263倍;PXR上调至455、506、298倍;Western blot结果,地格达-4各给药组对PXR具有显著的下调、CAR具有显著的上调作用(P0.01)。结论:幽门结扎能引起急性肝损伤,且能改变对核受体PXR和CAR表达的变化。地格达-4通过PXR和CAR膜蛋白表达变化,引起保护肝脏,促进胆汁分泌功能。
[Abstract]:Objective: to study the effect of Digelda-4 on the treatment of pyloric ligation of liver injury through the change of expression of PXRG and CAR.Methods: 50 rats were divided into 5 groups: normal group and model group. The rat liver injury model was established by pyloric ligation in 3 doses of digerda-4 (1.86 ~ 3.71 ~ 7.43 g 路kg ~ (-1) 路kg ~ (-1)). The changes of PXRCAR expression in rat liver and the sensitivity of liver injury in serum and liver tissue were detected by real-time fluorescence quantitative chain polymerase reaction (RTPCRN) and Western blot assay after intragastric administration for 15 days. Results: compared with the normal group, the expression of PXRCAR and the sensitivity of PXRCAR in serum and liver tissue were measured. In the model group, the serum TBA of alt BILD _ 2 and BILT _ 3 increased significantly, and the GSH-PX of liver tissue decreased significantly (P _ (0.01), P _ (0.01) P _ (0.01)), the peritoneal membrane had obvious yellow color, hydrocele, yellowish urine and naked eye to observe liver congestion, morphological observation showed that hepatic cells had obvious fat change and edema. Compared with the model group, the TBA of BILD _ 2 and BILT _ 3 in each administration group decreased significantly (P 0.01) and GSH-PX increased significantly (P 0.01). The morphology of abdominal cavity and liver was improved by RT-PCR. CAR of nuclear receptor was up-regulated to 9 739 ~ 3 066 ~ 1 263 times. The results of Western blot showed that digerda-4 could significantly down-regulate the expression of PXR. Conclusion: pyloric ligation can induce acute liver injury. [WT5 "HZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5BZ] the results of Western blot were as follows: 1. It can also change the expression of PXR and CAR in nuclear receptors. Digoda-4 can protect the liver and promote bile secretion through the changes of PXR and CAR membrane protein expression.
【作者单位】：内蒙古民族大学蒙医药学院;内蒙古民族大学附属医院;内蒙古民族大学医学院;
【基金】：国家自然科学基金项目(No.81360673)~~
【分类号】：R29

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