电针不同穴位组合对功能性腹泻大鼠空肠肠嗜铬细胞和5-HT的影响研究

发布时间：2018-03-28 16:44

本文选题：电针　切入点：功能性腹泻　出处：《陕西中医药大学》2016年硕士论文

【摘要】：目的:运用一定参数的电针,干预功能性腹泻模型大鼠的不同穴位组合,观察大鼠的各种腹泻指标及空肠中肠嗜铬细胞的形态数量、5-HT分泌量及TPH的表达量的不同变化,探索电针干预功能性腹泻的调节机制,筛选出电针干预功能性腹泻疗效较好的穴位组合。方法:选用20只SD大鼠,随机分为2组:空白组10只、模型组10只。空白组不做任何处理,模型组给以3ml/只番泻叶煎液灌胃,1次/天,连续灌胃14天。灌胃结束后,对两组大鼠继续以正常饲食、饮水,连续观察14d,至第28d造模结束,取材,观察综合评定各项指标。选用50只SD大鼠,随机分为5组:空白组、模型组、电针1组、电针2组、电针3组。模型组、电针1组、电针2组、电针3组运用上述腹泻模型造模方法造模,造模结束后,进行电针干预观察,空白组、模型组不予以电针,电针组(电针1组、电针2组、电针3组)给予电针干预1个疗程(1次/天,10天/疗程)。疗程结束后,截取大鼠的空肠组织,检测其肠嗜铬细胞数量、5-HT的分泌量及TPH的表达量。结果:番泻叶煎液灌胃法制备的功能性腹泻大鼠模型,在通过体重增长率、腹泻指数、稀便率、稀便级、浸银染色等方面的观察和综合评定后,证明腹泻模型大鼠的制备是成功的。造模结束后,与空白组对比,模型组大鼠在空肠部位中的肠嗜铬细胞数量和5-HT的分泌量、TPH的表达量均有所升高。电针干预后,与空白组、模型组比较,电针三组均可降低空肠中肠嗜铬细胞的数量、5-HT分泌量及TPH的表达量,电针1组(天枢配大肠俞)对空肠肠嗜铬细胞数量、5-HT的分泌量、TPH的表达量影响显著,电针2组(曲池配上巨虚)和电针3组(天枢配大肠俞+曲池配上巨虚)影响不显著。结论:1.运用番泻叶煎液灌胃制备的功能性腹泻大鼠,模型稳定,造模成功。2.空肠和回肠是番泻叶腹泻模型造模的作用部位,空肠为主要作用部位。3.造模及电针干预过程中,嗜铬细胞的数量、5-HT的分泌量及TPH的表达量与功能性腹泻有相关性联系。4.电针干预相关穴位,通过减少肠嗜铬细胞数量、5-HT释放量及TPH表达量,致使信号传导减弱,起到治疗作用。电针1组影响显著,即天枢配大肠俞为治疗功能性腹泻的较好穴位组合,电针2组和电针3组影响不显著。
[Abstract]:Objective: to observe the effects of electroacupuncture on different acupoints combination in rats with functional diarrhea, and to observe the changes of various diarrhea indexes and the morphologic quantity of chromaffin cells in jejunum and the expression of TPH. To explore the regulating mechanism of electroacupuncture intervention on functional diarrhea and to screen out the acupoint combination of electroacupuncture for functional diarrhea. Methods: 20 SD rats were selected and randomly divided into 2 groups: blank group (n = 10), control group (n = 10), control group (n = 10), and control group (n = 10). Ten rats in the model group. The blank group was given 3ml/ Senna leaf decoction once a day for 14 days. After the gastric administration, the rats in the two groups continued to be fed with normal diet, drinking water and observed continuously for 14 days until the end of the 28 th day. 50 Sprague-Dawley rats were randomly divided into 5 groups: blank group, model group, electroacupuncture group 1, electroacupuncture group 2, electroacupuncture group 3, model group, electroacupuncture group 1, electroacupuncture group 2. Three groups of electroacupuncture were used to model the diarrhea model. After the model was made, the intervention of electroacupuncture was observed. The blank group and the model group were not given electroacupuncture, and the electroacupuncture group (group 1 and group 2) were treated with electroacupuncture. Electroacupuncture group (group 3) was given electroacupuncture intervention for one course of treatment (10 days per day). After the course of treatment, the jejunum tissue of rats was cut off. Results: the rat model of functional diarrhea was made by gastric perfusion of Senna leaf decoction. Results: the rat model of functional diarrhea was induced by body weight growth rate, diarrhea index, fecal rate and fecal grade. After the observation and comprehensive evaluation of silver staining and other aspects, it was proved that the preparation of diarrhea model rats was successful. After the establishment of the model, it was compared with the blank group. The number of chromaffin cells in jejunum and the secretion of 5-HT in model group were increased. Electroacupuncture could decrease the secretion of 5-HT and the expression of TPH in jejunum. The effect of electroacupuncture on the secretion of 5-HT and the expression of 5-HT in jejunal chromaffin cells was significant in group 1 (Tianshu combined with Daichang Shu). The effects of electroacupuncture group 2 (zhuchi plus great asthenia) and electroacupuncture group 3 (Tianshu group combined with Dachang Yu koji group on great asthenia) were not significant. Conclusion: 1. The model of functional diarrhea rats prepared by gastric perfusion with Senna leaf decoction was stable. 2. Jejunum and ileum are the acting sites of the model of Senna leaf diarrhea, and jejunum is the main part of the model. 3. In the process of model making and electroacupuncture intervention, The number of chromaffin cells and the expression of TPH were related to the functional diarrhea. 4. Electroacupuncture interfered with the related acupoints. By reducing the number of chromaffin cells and the expression of TPH, the signal transduction was weakened. The effect of electroacupuncture group 1 was significant, that is, Tianshu combined with Dachang Shu was a better combination of acupoints for the treatment of functional diarrhea, but the effect of electroacupuncture group 2 and electroacupuncture group 3 was not significant.
【学位授予单位】：陕西中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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