桃核承气汤对慢性肾衰大鼠肾组织BMP-7及MMP-2表达影响的研究

发布时间：2018-03-29 20:51

本文选题：桃核承气汤　切入点：肾间质纤维化　出处：《福建中医药大学》2016年硕士论文

【摘要】：目的：通过观察桃核承气汤对5/6肾切除CRF大鼠肾组织BMP-7、MMP-2 及 TIMP-2表达的影响,探讨该方治疗CRF的疗效机制,为临床治疗CRF提供思路和实验依据。方法：60只雄性SD大鼠,随机分为空白组、假手术组、模型组、对照组和治疗组,每组各12只。空白组不予手术,假手术组仅做双肾被膜剥离,其余3组均行5/6肾切除手术,建立CRF大鼠模型。术后4周每天上午分别用桃核承气汤、尿毒清颗粒给予治疗组、对照组灌胃,其余3组分别予等量生理盐水灌胃。8周后腹主动脉采血检测各组大鼠RBC、HB、血清Scr、BUN、血β2-MG,收集尿液检测24h尿蛋白、尿β2-MG、尿NAG、尿RBP。取各组大鼠肾组织,分别检测肾组织BMP-7、MMP-2、TIMP-2蛋白和BMP-7、MMP-2mRNA的表达,并进行病理观察。结果： (1)模型组大鼠的RBC计数和HB含量均显著降低,而血清Scr、BUN、血清β2-MG、24h尿蛋白定量、尿β2-MG、尿NAG和尿RBP均显著升高(P0.05)；肾组织BMP-7、MMP-2蛋白及nRNA的表达均显著降低,而TIMP-2蛋白的表达显著升高(P0.05)；光镜下肾间质纤维明显增生。 (2)治疗组和对照组大鼠的RBC计数和HB含量均显著升高,而血清Scr、B JN、血清β2-MG、24h尿蛋白定量、尿β2-MG、尿NAG和尿RBP均显著降低(P0.05)；肾组织BMP-7、MMP-2蛋白及mRNA的表达均显著升高,而TIMP-2蛋白的表达却显著降低(P0.05)；光镜下两组肾间质纤维化程度均较模型组轻；除血常规外,治疗组疗效明显比对照组好(P0.05)结论： (1)桃核承气汤通过纠正肾性贫血,促进代谢产物的排出,改善肾小球和肾小管的功能,减轻肾脏的病变,从而延缓CRF的进展。(2)桃核承气汤治疗CRF的机制,可通过上调BMP-7、MMP-2蛋白及mRNA的表达,而下调TIMP-2蛋白的表达,以减少ECM的过度沉积,从而减轻肾间质纤维化。(3)除血常规外,与尿毒清颗粒相比,桃核承气汤治疗CRF的疗效更佳。
[Abstract]:Objective: to observe the effect of Taohe Chengqi decoction on the expression of BMP-7 and MMP-2 and TIMP-2 in renal tissue of CRF rats after 5 / 6 nephrectomy, and to explore the mechanism of its therapeutic effect on CRF, and to provide the thought and experimental basis for clinical treatment of CRF. Methods: 60 male Sprague-Dawley rats were selected. They were randomly divided into blank group, sham operation group, model group, control group and treatment group with 12 rats in each group. The CRF rat model was established. The rats were treated with Taohe Chengqi decoction and Niaoduqing granules respectively in the morning of 4 weeks after operation, and the control group were given intragastric perfusion. The other three groups were fed with normal saline for 8 weeks. The blood samples from abdominal aorta of each group were collected to detect RBCU HBs, serum Scrnb and 尾 2-MG. urine samples were collected to detect 24 hours urine protein, urine 尾 2-MGG, urinary nag and urine RBP.Renal tissues of each group were taken out. The expression of MMP-2TIMP-2 protein and BMP-7MGG mRNA in renal tissue were detected, and the pathological observation was made. Results: the RBC count and HB content in the model group were significantly decreased, while the serum Scr-BUNand the 24-hour urine protein of 尾 2-MGG were measured. Urinary 尾 2-MG, urinary NAG and urinary RBP increased significantly (P 0.05), and the expression of BMP-7 MMP-2 and nRNA decreased significantly in renal tissue. However, the expression of TIMP-2 protein was significantly increased (P 0.05), renal interstitial fiber proliferation was observed under light microscope. The RBC count and HB content of rats in treatment group and control group were significantly increased, while serum Scr-B JN and serum 尾 2-MGG 24-hour urinary protein were measured. Urinary 尾 2-MG, urinary NAG and urinary RBP decreased significantly the expression of BMP-7 MMP-2 and mRNA in renal tissue, while the expression of TIMP-2 protein significantly decreased P0.05.The degree of renal interstitial fibrosis in the two groups was lighter than that in the model group under light microscope, except for the blood routine, the renal interstitial fibrosis degree of the two groups was lower than that of the model group, and the renal interstitial fibrosis degree of the two groups was lower than that of the model group. Conclusion: Taohe Chengqi decoction can promote the excretion of metabolites, improve the function of glomeruli and renal tubules, and alleviate the pathological changes of kidney by correcting renal anemia, improving the function of glomeruli and tubules, and improving the function of renal tubules in the treatment group. Therefore, the mechanism of Taohe Chengqi decoction in treating CRF can down-regulate the expression of TIMP-2 protein by up-regulating the expression of BMP-7, MMP-2 and mRNA, so as to reduce the excessive deposition of ECM and alleviate the renal interstitial fibrosis. Compared with Niaoduqing granule, Taohe Chengqi decoction has better effect on CRF.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R277.5

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