方氏头针对血管性痴呆大鼠海马组织中炎性因子TNF-α表达的影响

发布时间：2018-03-29 23:07

本文选题：方氏头皮针　切入点：血管性痴呆　出处：《中国中医基础医学杂志》2017年06期

【摘要】：目的:观察各组大鼠海马组织中炎性因子TNF-α表达的变化,探讨方氏头针治疗VD的作用机理。方法:将40只健康大鼠随机分为正常组、假手术组、模型组和头针组4组各10只,通过改良的4-VO法制备VD模型,随后头针组进行选穴针刺,余3组正常喂养,10 d后采用Morris水迷宫检测各组大鼠学习记忆能力的变化,ELLSA检测大鼠海马区TNF-α表达的变化。结果:与正常组比较,模型组大鼠的平均逃避潜伏期及首次跨越原象限平台的时间均有明显延长,海马组织中TNF-α表达也明显升高,而经头皮针治疗后,大鼠的平均逃避潜伏期及首次跨越原象限平台的时间明显缩短,TNF-α表达明显降低,正常组与假手术组比较差异无统计学意义。结论:方氏头针可以有效改善VD大鼠的学习记忆能力,这可能与方氏头皮针可以调控大鼠海马组织中TNF-α的表达有关。
[Abstract]:Objective: to observe the expression of inflammatory factor TNF- 伪 in hippocampus of rats in each group, and to explore the mechanism of Fangshi scalp acupuncture in treating VD. Methods: forty healthy rats were randomly divided into normal group, sham operation group, model group and scalp acupuncture group (n = 10 each). VD model was established by modified 4-VO method, and then acupuncture was performed in scalp acupuncture group. Morris water maze was used to detect the changes of learning and memory ability of rats in the remaining 3 groups after 10 days of normal feeding. Results: compared with the normal group, the expression of TNF- 伪 in hippocampus was detected by ELLSA. In the model group, the average escape latency and the first time to cross the original quadrant platform were significantly prolonged, and the expression of TNF- 伪 in the hippocampus was also significantly increased after scalp acupuncture treatment. The mean escape latency and the first time to cross the original quadrant platform in rats were significantly shortened and the expression of TNF- 伪 was significantly decreased. Conclusion: Fangshi scalp acupuncture can effectively improve the learning and memory ability of VD rats, which may be related to the regulation of TNF- 伪 expression in hippocampal tissue of VD rats by Fangshi scalp acupuncture.
【作者单位】：成都中医药大学针灸推拿学院;陕西中医学院;西安市中医医院针灸推拿科;
【分类号】：R245

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