水药金耳环多糖对实验性2型糖尿病模型大鼠糖脂代谢、肾功能及氧化应激的影响

发布时间：2018-04-03 15:32

  本文选题：水药　切入点：金耳环多糖　出处：《中国药房》2017年31期

【摘要】：目的:研究水药金耳环多糖对实验性2型糖尿病模型大鼠糖脂代谢、肾功能及氧化应激的影响,为金耳环多糖的开发利用提供参考。方法:将60只SD大鼠随机分为正常对照组、模型对照组、阳性对照组(厄贝沙坦片,0.02 g/kg)和金耳环多糖低、中、高剂量组(以生药计分别为2、4、8 g/kg),每组10只。除正常对照组外,其余各组大鼠均ip链脲佐菌素75 mg/(kg·d)复制糖尿病大鼠模型。成模后,各给药组大鼠ig相应药物,正常对照组和模型对照组大鼠ig等体积5%羧甲基纤维素钠溶液,每天2次,连续42d。给药结束后,采用紫外分光光度计检测肝组织中肝糖原含量;采用全自动生化分析仪检测大鼠24 h尿量、24 h尿蛋白含量,血清中三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、肌酐(Cr)、尿素氮(BUN)水平,以及肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、超氧化物歧化酶(ROS)、丙二醛(MDA)水平。结果:与正常对照组比较,模型对照组大鼠肝组织中肝糖原含量减少(P0.05);24 h尿量、24 h尿蛋白量增加(P0.05);血清中TG、TC、LDL-C水平升高(P0.05),HDL-C水平降低(P0.05);肾组织中SOD、CAT、GSH-Px水平降低(P0.05),ROS、MDA水平升高(P0.05)。与模型组比较,除肝组织中肝糖原含量、血清中HDL-C水平以及金耳环多糖低剂量组大鼠24 h尿量改善不显著外,其余各给药组大鼠上述指标均得到明显改善(P0.05)。结论:金耳环多糖可调节实验性2型糖尿病模型大鼠的血脂代谢紊乱、改善大鼠肾功能和提高肾抗氧化能力。
[Abstract]:Objective: to study the effects of polysaccharides on glucose and lipid metabolism, renal function and oxidative stress in experimental type 2 diabetic rats, and to provide reference for the development and utilization of polysaccharides from gold earrings.Methods: sixty Sprague-Dawley rats were randomly divided into normal control group, model control group, positive control group (Irbesartan 0.02 g 路kg ~ (-1)) and low, medium and high dose group (2: 48 g 路kg ~ (-1) 路kg ~ (-1)) of irbesartan tablets (n = 10).The diabetic rats were induced by streptozotocin (75 mg/(kg d).After the model was established, the rats of each group were given intravenously, and the rats of normal control group and model control group were given 5% sodium carboxymethyl cellulose solution twice a day for 42 days.After administration, the content of liver glycogen in liver tissue was detected by ultraviolet spectrophotometer, and the urine protein content in 24 h urine of rats was detected by automatic biochemical analyzer.Serum triacylglycerol (TGN), cholesterol triglyceride (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), creatinine (Cr), urea nitrogen bun (BUNA),And the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PxN), catalase (CATX), superoxide dismutase (SOD) and malondialdehyde (MDA) in renal tissue.缁撴灉:涓庢�ｅ父瀵圭収缁勬瘮杈�,妯″瀷瀵圭収缁勫ぇ榧犺倽缁勭粐涓�鑲濈硸鍘熷惈閲忓噺灏�(P0.05);24 h灏块噺,24 h灏胯泲鐧介噺澧炲姞(P0.05);琛，

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