壮药竹节蓼的质量标准研究

发布时间：2018-04-05 23:04

  本文选题：壮药　切入点：竹节蓼　出处：《中国药房》2017年21期

【摘要】：目的:建立壮药竹节蓼的质量标准。方法:采用薄层色谱法(TLC)对药材样品进行定性鉴别;采用高效液相色谱法对药材样品中槲皮苷进行含量测定:色谱柱为Agilent Zorbax SB-C_(18),流动相为乙腈-0.1%冰醋酸溶液(20∶80,V/V),流速为1.0mL/min,检测波长为360 nm,柱温为30℃,进样量为10μL;参照2015年版《中国药典》测定药材样品水分、灰分和浸出物的含量。结果:竹节蓼药材的TLC图斑点清晰,分离度好。槲皮苷检测质量浓度线性范围为0.023~0.46 mg/mL(r=0.999 1);精密度、稳定性、重复性试验的RSD2.0%;加样回收率为98.01%~103.05%(RSD=1.71%,n=6)。药材样品水分、总灰分、酸不溶性灰分、浸出物和槲皮苷含量分别为6.39%~9.78%、2.82%~8.07%、0.02%~0.27%、14.14%~28.45%、0.09%~0.50%。结论:该研究所建标准可用于壮药竹节蓼的质量控制。
[Abstract]:Objective: to establish the quality standard of Polygonum gondii.Methods: TLC was used to identify the samples.The content of quercetin in the sample was determined by high performance liquid chromatography (HPLC). The chromatographic column was Agilent Zorbax SB-CX 18, the mobile phase was acetonitrile-0.1% glacial acetic acid solution, the flow rate was 1.0 mL / min, the detection wavelength was 360 nm, the column temperature was 30 鈩，

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