蒙医三根平衡针对慢性应激抑郁模型大鼠海马神经元形态及BDNF-CREB信号通路的影响

发布时间：2018-04-06 22:39

本文选题：抑郁　切入点：蒙医三根平衡针　出处：《中华中医药杂志》2017年08期

【摘要】：目的:观察蒙医三根平衡针对慢性应激抑郁模型大鼠海马神经元形态及BDNF-CREB信号通路的影响,以期探讨蒙医三根平衡针抗抑郁作用的机制。方法:雄性SD大鼠随机分为空白组、模型组、药物组、蒙医银针组、蒙医三根平衡针组和针药结合组,每组8只。除空白组外,每组大鼠孤养,进行不可预知的应激刺激,并接受不同的治疗。28d后检测行为学改变;断头、取材,观察CA3区神经元形态;检测海马BDNF、CREB的表达。结果:与空白组比较,模型组大鼠水平及垂直运动次数明显减少(P0.05)。与模型组比较,蒙医三根平衡针组、药物组及针药结合组水平及垂直运动次数明显增加(P0.05),海马CA3区神经元锥体细胞排列较有序、神经元胞膜较完整;海马BNDF、CREB的表达升高(P0.05)。结论:蒙医三根平衡针治疗抑郁症的作用机制,与神经元保护及上调海马BDNF-CREB表达有关。
[Abstract]:Aim: to observe the effects of three branches of Mongolian medicine on hippocampal neuronal morphology and BDNF-CREB signal pathway in rats with chronic stress depression, in order to explore the mechanism of antidepressant effect of three balanced acupuncture in Mongolian medicine.Methods: male Sprague-Dawley rats were randomly divided into blank group, model group, drug group, Mongolian medicine silver needle group, Mongolian medicine three balanced acupuncture group and acupuncture combined group with 8 rats in each group.Except the blank group, rats in each group were isolated and stimulated by unpredictable stress. The behavioral changes were detected after different treatment for 28 days; the morphology of neurons in the CA3 area was observed by cutting off the head, and the expression of BDNF / CREB in hippocampus was detected.Results: compared with the blank group, the number of horizontal and vertical movements in the model group decreased significantly (P 0.05).Compared with the model group, the horizontal and vertical motion times of the three balanced acupuncture groups, the medicine group and the combined acupuncture and medicine group, increased significantly, the pyramidal cells in the hippocampal CA3 region were arranged orderly and the neuronal membrane was intact, and the expression of BNDF- CREB in the hippocampus was increased (P0.05).Conclusion: the mechanism of treatment of depression by three balanced acupuncture in Mongolian medicine is related to neuronal protection and up-regulation of hippocampal BDNF-CREB expression.
【作者单位】：内蒙古自治区国际蒙医医院;内蒙古自治区精神卫生中心;北京中医药大学针灸推拿学院;
【基金】：国家自然科学基金项目(No.81360576) 内蒙古自治区自然科学基金项目(No.2015MS08130)~~
【分类号】：R29

【参考文献】