刺糖对酒精性肝病TNF-α和TLR4表达及病理变化的影响

发布时间：2018-04-09 01:07

本文选题：刺糖　切入点：肿瘤坏死因子-α　出处：《动物医学进展》2017年06期

【摘要】：探讨刺糖对酒精性肝病(Alcoholic liver disease,ALD)小鼠肝组织TNF-α和TLR4表达及组织病理变化的影响。将42只小鼠随机分成空白组、模型组、水飞蓟宾阳性组、刺糖高、中、低剂量组。模型组以500mL/L酒精造模,空白对照组(15mL/kg生理盐水)、刺糖高、中、低(600、300、150mg/kg)、水飞蓟宾(300mg/kg)灌胃,每日药物处理后用500mL/L酒精(10mL/kg)灌胃2次(分别为药物处理后2h和药物处理后10h),连续3d,末次灌胃酒精12h后,取肝脏提取mRNA,制备肝脏标本进行HE染色,实时荧光定量PCR检测肝组织TNF-α和TLR4mRNA表达。结果表明,与模型组相比,小鼠肝脏中TNF-α和TLR4mRNA表达水平明显降低。各刺糖给药组均抑制酒精引起的TNF-α和TLR4基因mRNA表达水平的增强。刺糖高、中、低剂量组均能够改善酒精所引起的肝脏病理变化。可推出刺糖对酒精引起的小鼠肝损伤有一定的保护作用,其作用机制可能与抑制TNF-α和TLR4基因mRNA表达有关。
[Abstract]:To investigate the effect of prickly sugar on the expression of TNF- 伪 and TLR4 in the liver tissue of alcoholic liver disease (ALD) mice and its histopathological changes.42 mice were randomly divided into blank group, model group, silythistle Binyang group, high, middle and low dose groups.The model group was made with 500mL/L alcohol, and the blank control group was treated with 15ml / kg normal saline, 600,300mg / kg silybin and 300mg / kg silybin.Two times (2 h after drug treatment and 10 h after drug treatment) were treated with 500mL/L alcohol (10 mL / kg) for 3 consecutive days. After the last administration of alcohol for 12 h, mRNAs were extracted from the liver, and the liver specimens were prepared for HE staining.The expression of TNF- 伪 and TLR4mRNA in liver tissue was detected by real-time fluorescence quantitative PCR.The results showed that compared with the model group, the expression of TNF- 伪 and TLR4mRNA in the liver of mice decreased significantly.All the prickly sugar groups inhibited the increase of mRNA expression of TNF- 伪 and TLR4 gene induced by alcohol.High, medium and low doses of prickly sugar could improve the pathological changes of liver induced by alcohol.It can be concluded that prickly sugar has a protective effect on the liver injury induced by alcohol in mice, and its mechanism may be related to the inhibition of the expression of TNF- 伪 and TLR4 gene mRNA.
【作者单位】：新疆农业大学动物医学学院;西北农林科技大学动物医学院;
【基金】：国家自然科学基金项目(31460677)
【分类号】：R29

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