针刺手厥阴经穴对心肌缺血再灌注损伤大鼠CRTmRNA表达率的影响

发布时间：2018-04-10 21:05

  本文选题：针刺 + 心肌缺血再灌注损伤　； 参考：《中国中医基础医学杂志》2017年01期

【摘要】：目的:观察电针手厥阴经"内关""郄门"穴对缺血再灌注损伤过程大鼠心肌组织CRTmRNA表达率的影响,探讨经穴与脏腑间的特异性联系。方法:50只Wistar大鼠根据随机数字表法分为假手术组、缺血-再灌注模型组、针刺"内关"治疗组、针刺"郄门"治疗组以及针刺"合谷"对照组5组各10只。除假手术组外,其他4组均制备缺血-再灌注动物模型,假手术组穿线但不结扎冠状动脉。"内关"、"郄门"、"合谷"3组穴位针刺20 min后,结扎冠状动脉左前降支40 min,再次针刺上述穴位20 min松扎,恢复冠脉灌流60 min后摘取心脏取心尖部组织,采用RT-PCR方法测定CRTmRNA表达率,实验过程中心电图(ECG)全程监测。结果:假手术组CRTmRNA的表达很低,模型组与"内关"组、"郄门"组比较,CRTmRNA表达率下降非常明显;模型组与"合谷"组比较,CRTmRNA表达率比较差异无统计学意义;"内关"组与"郄门"组比较差异无统计学意义。结论:针刺手厥阴经穴可有效促进CRTmRNA的表达,提高CRTmRNA的表达率,减轻胞浆钙超载对心肌细胞的损伤。
[Abstract]:Methods Fifty Wistar rats were randomly divided into sham-operation group, ischemia-reperfusion model group, acupuncture "Neiguan" treatment group, acupuncture "Ximen" treatment group and acupuncture "Hegu" control group.Except sham-operation group, all the other four groups were used to establish ischemia reperfusion animal model, sham-operation group perforated but did not ligate coronary artery. "The electrocardiogram (ECG) was monitored in the whole course of the experiment.Results: the expression of CRTmRNA in sham operation group was very low, and the expression rate of CRTmRNA in model group was significantly lower than that in "Neiguan" group and "Ximen" group.There was no significant difference in CRT mRNA expression between model group and Hegu group, but there was no significant difference between "Neiguan" group and "Ximen" group.
【作者单位】： 山西中医学院针灸推拿学院;山西中医学院附属医院;山西医科大学第二医院;
【基金】：国家自然科学基金资助项目(30873240)-针刺对缺血再灌注损伤心肌细胞CaMKⅡ信号传递作用及Akt心肌保护作用的研究 山西省自然科学基金资助项目(2010011056-1)-针刺激活Akt信号通路对再灌注损伤心肌细胞凋亡干预机制的研究
【分类号】：R245
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本文编号：1732909