维药孜然不同提取物抗氧化及对α-葡萄糖苷酶抑制的研究

发布时间：2018-04-12 19:52

  本文选题：孜然 + 提取物　； 参考：《新疆大学》2016年硕士论文

【摘要】：孜然(Cuminum cyminum L.)在维药中果实入药,用以治疗消化不良和胃寒腹痛等。孜然果实主要含有挥发性和非挥发性两部分成分,在挥发性成分,烯烃类化合物较多,非挥发性成分主多要有酚类、黄酮类、糖类、脂类、蛋白类等化合物。黄酮及多酚类化合物具有广泛的生物活性,如抗氧化、抗菌、抗病毒、抗糖尿病等。本文采用溶剂提取法获得孜然总酚、总黄酮,并对孜然总酚提取工艺进行了优化;同时采用有机溶剂萃取法获得孜然果实不同提取部位,采用抗氧化相应评价方法(DPPH法、ABTS法、OH·法、铁氰化钾还原法)对孜然总酚、总黄酮及不同提取物抗氧化活性进行研究,同时利用酶法,对孜然总酚、总黄酮及不同提取物具有抑制α-葡萄糖苷酶性进行探讨。孜然总酚含量采用Folin-Ciocalteu比色法测定,孜然总黄酮含量应用硝酸铝显色法测定,孜然总酚含量测定采用响应面法进行工艺优化,初步纯化了孜然总酚和总黄酮。结果如下:(1)孜然总酚提取优化条件为:乙醇浓度70%、料液比1:20、超声温度60℃、超声时间60 min。在此工艺条件下,孜然总酚的含量为13.812 mg/g,高出预测值4.5%。大孔吸附树脂法对孜然总酚粗提物初步纯化,孜然总酚含量从12.044mg/m L上升至约20.186mg/m L,显示大孔树脂对孜然总酚具有一定的纯化效果。纯度从儿茶素当量34%上升至59%。孜然总黄酮含量为27.7+1.3μg/m L;醇溶性黄酮含量为25.3+0.9μg/m L;水溶性黄酮含量为13.5+0.3μg/m L。(2)抗氧化评价中DPPH自由基清除能力石油醚部位最强,依次为Vc石油醚提取物氯仿提取物乙酸乙酯提取物正丁醇提取物乙醇提取物纯化后总酚孜然总酚纯化后总黄酮总黄酮;ABTS自由基清除能力(浓度范围1-25mg/m L)乙酸乙酯部位最强:依次为Vc乙酸乙酯提取物正丁醇提取物纯化后总黄酮纯化后总酚氯仿提取物石油醚提取物孜然总酚乙醇提取物总黄酮;OH·自由基清除能力乙酸乙酯部位最强:依次为Vc乙酸乙酯提取物正丁醇提取物石油醚提取物纯化后总酚乙醇提取物孜然总酚氯仿提取物纯化后总黄酮总黄酮;总还原力实验中,孜然各提取物总还原力(浓度范围1-25mg/m L)最强的为正丁醇部位。以上结果可为今后从孜然中分离出抗氧化活性物质一定的数据基础。(3)采用α-葡萄糖苷酶抑制实验,研究孜然不同提取物抗糖尿病活性,对α-葡萄糖苷酶的抑制活性,孜然总酚阳性对照阿卡波糖孜然乙醇提取物孜然总黄酮孜然氯仿提取物孜然石油醚提取物孜然乙酸乙酯提取物孜然正丁醇提取物,孜然精油IC50值为72.41μL/m L。酶法实验结果显示,孜然不同提取物都具有一定的抑制α-葡萄糖苷酶活性,且孜然总酚对于α-葡萄糖苷酶抑制效果最强,可为孜然的降血糖活性的进一步研究提供数据参考。
[Abstract]:Cuminum cyminum L.The fruit is used in the treatment of dyspepsia and cold stomach pain.Cumin fruits mainly contain volatile and non-volatile components, in volatile components, olefins are more, non-volatile components are mainly phenols, flavonoids, carbohydrates, lipids, proteins and other compounds.Flavonoids and polyphenols have a wide range of biological activities, such as anti-oxidation, antibacterial, anti-virus, anti-diabetes and so on.In this paper, the total phenol and flavonoids of cumin were obtained by solvent extraction, and the extraction process of total phenol from cumin was optimized, and the different extraction parts of cumin fruit were obtained by organic solvent extraction.The antioxidant activities of total phenol, total flavonoids and different extracts of cumin were studied by DPPH method, Abts method (OH method) and potassium ferricyanide reduction method. The antioxidant activities of cumin total phenol, total flavonoids and different extracts were studied by enzymatic method.The inhibitory activity of total flavonoids and different extracts on 伪-glucosidase was studied.The total phenol content of cumin was determined by Folin-Ciocalteu colorimetry, the total flavone content of cumin was determined by aluminum nitrate coloration method, the total phenol content of cumin was optimized by response surface method, and the total phenol and flavonoids of cumin were preliminarily purified.The results showed that the optimum extraction conditions of cumin total phenol were as follows: ethanol concentration 70%, ratio of material to liquid 1: 20, ultrasonic temperature 60 鈩，

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