恐伤孕鼠对其仔鼠颅骨培养成骨细胞Cx43、BGP表达的影响

发布时间：2018-04-12 22:23

本文选题：恐伤孕鼠 + 左归丸　；参考：《中华中医药学刊》2017年11期

【摘要】：目的:从先天肾虚影响颅骨培养成骨细胞Cx43、BGP表达情况入手,研究其影响骨早期发育的机制,并论证补肾填精法的作用。方法:采用模拟地震振动、光电刺激复合应激恐吓孕鼠造成子代先天肾虚。将孕鼠分为正常、模型、补肾3组,补肾组灌服左归丸浓缩剂。取各组新生仔鼠颅盖骨制取培养成骨细胞,观察细胞生长形态、ALP活性测定、RT-PCR检测第7、14、21天Cx43和BGPmRNA表达情况、免疫荧光染色检测第21天Cx43蛋白表达。结果:模型组仔鼠成骨细胞培养第5、7天ALP活性降低;第14、21天Cx43、BGPmRNA表达减少;第21天免疫荧光检测Cx43蛋白表达减少。上述指标与另两组比较差异显著(P0.05)。结论:先天肾虚导致仔鼠颅骨成骨细胞分化成熟过程中Cx43、BGP表达减少,这是其影响骨早期发育的重要机制之一。左归丸补肾填精治疗可提高Cx43、BGP表达,保证成骨细胞正常功能,促进骨发育。
[Abstract]:Objective: to study the mechanism of the effect of congenital kidney deficiency on the expression of Cx43 BGP in osteoblasts of skull culture, and to demonstrate the effect of tonifying kidney and filling essence.Methods: simulated earthquake vibration and photoelectricity stimulation combined stress were used to intimidate pregnant rats to cause congenital kidney deficiency in their offspring.Pregnant rats were divided into three groups: normal, model, kidney tonifying group and kidney tonifying group.Cultured osteoblasts were obtained from calvaria of newborn rats in each group. The expression of Cx43 and BGPmRNA was detected by RT-PCR on day 7, 14 and 21, and the expression of Cx43 protein on day 21 by immunofluorescence staining.Results: in the model group, the activity of ALP was decreased on the 7th day, the expression of Cx43 BGP was decreased on the 14th day, and the expression of Cx43 protein was detected by immunofluorescence on the 21st day.The above indexes were significantly different from the other two groups (P 0.05).Conclusion: congenital deficiency of kidney leads to the decrease of Cx43 BGP expression during the differentiation and maturation of calvarial osteoblasts, which is one of the important mechanisms affecting the early development of bone.Zuo Gui Pill can improve the expression of Cx43 BGP, ensure the normal function of osteoblasts and promote bone development.
【作者单位】：湖北中医药大学中医临床学院;
【基金】：国家自然科学基金青年科学基金项目(81403285) 国家中医药管理局“周安方全国名老中医药专家传承工作室”建设项目([2014]20)
【分类号】：R271

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