壮药锡叶藤药材质量标准研究

发布时间：2018-04-14 12:00

  本文选题：锡叶藤 + 没食子酸　； 参考：《中华中医药学刊》2017年01期

【摘要】：目的:建立锡叶藤药材的质量标准。方法:对锡叶藤根的性状、显微进行鉴别;采用薄层色谱法(TLC)对锡叶藤进行定性鉴别;采用HPLC法测定药材中的没食子酸含量,色谱柱为Phenomenex C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸水溶液(5∶95),检测波长280 nm,流速1 m L·min-1,柱温30℃。结果:锡叶藤根的薄层鉴别鉴别特征明显,专属性强;没食子酸的进样量在0.026 8~1.342μg(r=0.999 9)范围内与峰面积积分值呈良好线性关系,平均加样回收率为99.65%(RSD=1.26%,n=6)。结论:所建标准可用于锡叶藤药材的质量控制。
[Abstract]:Objective: to establish the quality standard of Dioscorea tinctata.Methods: TLC was used to identify the characters of the root, TLC was used to determine the content of Gallic acid, and HPLC was used to determine the content of Gallic acid.The chromatographic column was Phenomenex C18 column (4.6mm 脳 250mm-1), the mobile phase was methanol-0.1% phosphoric acid aqueous solution (5: 95), the detection wavelength was 280nm, the flow rate was 1 mL 路min ~ (-1), the column temperature was 30 鈩，

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