苗族药了哥王不同提取物对正常大鼠肝毒性的影响

发布时间：2018-04-14 19:37

本文选题：了哥王 + 提取物　；参考：《中国实验方剂学杂志》2017年11期

【摘要】：目的:考察了哥王不同提取物对正常大鼠肝毒性的影响,并探讨其作用机制。方法:选用SD大鼠72只,随机分成空白组、乙醇提取物组、石油醚提取物组、乙酸乙酯提取物组、正丁醇提取物组和水部位提取物组,每组12只。不同给药组连续灌胃给药2周,每隔2日称量大鼠体重,于第15天,每组随机取6只,麻醉后腹主动脉取血,离心血液,取血清测定丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),碱性磷酸酶(ALP),总胆红素(T-BIL),总蛋白(TP),白蛋白(ALB),血糖(GLU),总胆固醇(CHOL),肌酐(Cr)。取肝组织,称其质量,计算脏器系数,并留取肝组织作病理学检查。每组余下的6只大鼠于第15天停止给药,正常供给饲料与水,恢复2周,观察大鼠恢复情况,于第28天进行上述相同指标的检测。结果:给药2周后,与空白组比较,乙醇提取物组和乙酸乙酯提取物组大鼠的肝脏系数明显增大(P0.05)。乙醇提取物组血清中ALT,AST,GLB明显升高,白蛋白/球蛋白(A/G),TP和ALB明显降低(P0.05);乙酸乙酯提取物组血清中ALP,T-BIL,GLU明显升高,A/G,TP和ALB明显降低(P0.05);石油醚提取物组血清中AST,AST/ALT,ALP,GLU明显升高,T-BIL,A/G,TP和ALB明显降低(P0.05,P0.01);正丁醇提取物组血清中AST/ALT,T-BIL明显升高,A/G明显降低(P0.05,P0.01);水部位提取物组血清中A/G明显降低(P0.05);乙醇提取物组、乙酸乙酯提取物组和石油醚提取物组大鼠肝组织能观察到不同程度的炎细胞浸润,出现一定的肝细胞脂肪变性和局灶性肝细胞坏死,其他组少见。停药恢复2周后,各组肝脏系数均接近于空白组;各组血清中肝功能生化指标基本恢复到空白组水平;各组大鼠肝组织细胞结构清晰,少见炎细胞浸润,未见脂肪变性和肝细胞坏死。结论:苗族药了哥王乙醇提取物组、乙酸乙酯提取物组对肝损伤较大,具有明显的肝毒性;石油醚提取物组对肝也有一定的损伤,但比乙醇提取物组和乙酸乙酯提取物组损伤程度小;正丁醇提取物组毒性较小,水部位提取物组无毒性;停药2周恢复后,各组大鼠反映肝功能的生化指标基本恢复到空白组水平,大鼠的肝损伤明显的减轻,表明了哥王的肝毒性具有一定的可逆性。
[Abstract]:Aim: to investigate the effects of different extracts of Koking on the hepatotoxicity of normal rats and its mechanism.Methods: Seventy-two SD rats were randomly divided into control group, ethanol extract group, petroleum ether extract group, ethyl acetate extract group, n-butanol extract group and water extract group with 12 rats in each group.Rats in different groups were given intragastric administration for 2 weeks and weighed every 2 days. On the 15th day, 6 rats in each group were randomly selected. Blood was taken from abdominal aorta after anesthesia and blood was centrifuged.Serum levels of alanine aminotransferase (alt), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (T-BILN), total protein (TP), albumin (Alb), blood glucose (GLU), total cholesterol (TC) and creatinine (Cr) were determined.Take the liver tissue, call its mass, calculate the organ coefficient, and leave the liver tissue for pathological examination.The remaining 6 rats in each group were stopped on the 15th day, fed with normal feed and water, and recovered for 2 weeks. The recovery of the rats was observed, and the same indexes were detected on the 28th day.Results: after 2 weeks of administration, compared with the blank group, the liver coefficient of the ethanol extract group and the ethyl acetate extract group increased significantly (P 0.05).In the ethanol extract group, the serum ALT AST GLB was significantly higher than that in the control group.Albumin / globulin A / G (TP) and ALB decreased significantly (P 0.05N), serum ALP T-BILN GLU in ethyl acetate extract group increased significantly (P 0.05) and ALB decreased significantly (P 0.05); serum AST / T (alt) ALPGLU significantly increased in petroleum ether extract group (P < 0.05); blood P0.05T (P 0.05) was decreased in butanol extract group (P < 0.05); blood P0.05P was significantly decreased in ethyl acetate extract group (P < 0.05); serum level of ALB was significantly decreased in butanol extract group (P < 0.01); serum level of ASTT / T (alt) was significantly increased in petroleum ether extract group (P < 0.05).In Qingzhong, AST / alt T-BIL was significantly increased and A-1 / G decreased significantly (P / P 0.05), P / G was significantly decreased in water extract group, and P / G was significantly decreased in water extract group; ethanol extract group was treated with ethanol extract.In the ethyl acetate extract group and the petroleum ether extract group, different degrees of inflammatory cell infiltration were observed in the liver tissue, and some fatty degeneration of liver cells and focal hepatocyte necrosis were observed in the other groups, which were rare in other groups.After 2 weeks of withdrawal, the liver coefficient of each group was close to that of the blank group; the biochemical indexes of liver function in the serum of each group returned to the level of the blank group; the structure of liver tissue cells in each group was clear, and the infiltration of inflammatory cells was rare.There was no fatty degeneration and hepatocyte necrosis.Conclusion: in the Miao nationality, the alcohol extract group of Koking was found to have great liver injury and obvious hepatotoxicity in ethyl acetate extract group, and the petroleum ether extract group also had some damage to the liver.The toxicity of n-butanol extract group was less than that of ethanol extract group and ethyl acetate extract group, the water extract group had no toxicity, and after 2 weeks of withdrawal, the toxicity of n-butanol extract group was less than that of ethyl acetate extract group.The biochemical indexes reflecting liver function in each group were basically restored to the level of blank group, and the liver injury of rats was obviously alleviated, indicating that the hepatotoxicity of elder brother Wang had certain reversibility.
【作者单位】：天津中医药大学;贵阳中医学院;国家苗药工程技术研究中心;
【基金】：国家自然科学基金项目(81303141) 国家中医药管理局中医药部门公共卫生服务项目([2015]136) 贵州省高校工程技术研究中心建设项目(黔教合KY字[2012]021号) 贵阳中医学院基金项目(贵中医科院内[2016]40号) 贵州省发展和改革委员会投资建设项目(黔发改投资[2015]542号)
【分类号】：R29

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