“正常体质-肾虚体质-肾虚证候”唾液代谢组学研究

发布时间：2018-04-14 21:53

本文选题：正常体质 + 肾虚体质　；参考：《中华中医药杂志》2017年11期

【摘要】：目的:探讨"正常体质-肾虚体质-肾虚证候"动态演变过程中唾液代谢物的变化。方法:根据中医"恐伤肾"理论,运用猫吓孕鼠的方法制作先天不足肾虚体质动物模型,运用肌肉注射氢化可的松方法制作肾虚证候动物模型,观察各组大鼠唾液代谢物的变化。结果:与正常体质对照组比较,肾虚体质模型组唾液中α-亚麻酸、γ-亚麻酸、十八碳三烯酸含量升高,肾虚证候模型组大鼠唾液中二十碳五烯酸、二高γ-亚麻酸、苯丙氨酸、牛磺酸、6-甲基腺嘌呤含量下降。与肾虚体质模型组比较,肾虚证候模型组大鼠唾液酪氨酸酶含量升高。结论:伴随着"正常体质-肾虚体质-肾虚证候"的动态演变过程,唾液中的代谢物成分发生了变化,进一步说明了中医"肾在液为唾"理论的科学性。
[Abstract]:Objective: to investigate the changes of salivary metabolites during the dynamic evolution of "normal constitution-kidney deficiency constitution-kidney deficiency syndrome".Methods: according to the theory of "kidney scares and kidney injury" in traditional Chinese medicine, the animal model of deficiency of kidney and the animal model of syndrome of kidney deficiency were made by injecting hydrocortisone intramuscularly.The changes of salivary metabolites in each group were observed.Results: compared with the normal constitution control group, the contents of 伪 -linolenic acid, 纬 -linolenic acid and octadectrienoic acid in saliva of kidney deficiency model group were increased, and the contents of eicosapentaenoic acid, dihigh 纬 -linolenic acid and phenylalanine in saliva of kidney deficiency syndrome model group were increased.The content of taurine 6-methyl adenine decreased.Compared with the kidney deficiency model group, the salivary tyrosinase content in the kidney deficiency syndrome group was increased.Conclusion: along with the dynamic evolution of "normal constitution, kidney deficiency constitution and kidney deficiency syndrome", the metabolites in saliva have changed, which further explains the scientific nature of the theory of "kidney is in the saliva".
【作者单位】：陕西中医药大学;西京学院;
【基金】：国家自然科学基金项目(No.81202614) 陕西省科技厅科研项目(No.2015JQ8291) 陕西省教育厅重点实验室科研计划项目(No.17JS032)~~
【分类号】：R22

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