甘丹油治疗婴幼儿湿疹的临床与实验研究

发布时间：2018-04-15 03:31

本文选题：婴幼儿湿疹 + 中药甘丹油　；参考：《南京中医药大学》2016年博士论文

【摘要】：目的：1观察甘丹油治疗婴幼儿湿疹的临床疗效。2通过小鼠实验研究观察甘丹油外用时对湿疹模型小鼠的消炎止痒作用,并且观察中药甘丹油对小鼠背部湿疹皮损中IL-2、1NF-α、IFN-γRa和小鼠血中CD4、CD8的免疫影响。方法：1动物的药效学研究部分,每日涂抹2,4-二硝基氯苯(2,4-DNCB)于动物的耳、背部皮肤,造其湿疹造模,另外用0.5%的4-氨基吡啶(4-AP)造其瘙痒模型,采用中药甘丹油进行治疗,观察小鼠舔体反应次数,比较小鼠两耳厚度、重量差、耳部皮肤组织中炎症细胞数值的变化,用免疫组化法检测各组小鼠背部皮损组织中的含量多少,及检测各组小鼠血中CD4.CD8淋巴细胞的数值高低。2 临床病例研究122例婴幼儿湿疹患者随机分为治疗组61例、对照组61例。治疗组采用中药甘丹油治疗,对照组采用丁酸氢化可的松软膏治疗。比较两组病情总积分改变,疗效前后对比,皮疹形态表现分值的改变。结果：1中药甘丹油治疗婴幼儿湿疹疗效确切。2中药甘丹油外用对湿疹造模的小鼠其皮损处有明显的消炎作用,其机理为润肤止痒,调节小鼠体内分泌紊乱的细胞因子等。结论：1临床病例研究结果表明,对照组各项指标明显优于治疗组,但从其复发率、副作用方面考量治疗组优于对照组。体现中药甘丹油治疗婴幼儿湿疹疗效较好,可作为理想的治疗婴幼儿湿疹药物。2小鼠药效学结果显示中药甘丹油外用治疗致小鼠耳廓肿胀模型、背部湿疹造模均有显著的抑制作用,具有抗炎消肿作用；对2,4-DNCB致小鼠背部皮肤瘙痒模型有明显的抑制作用,其止痒作用与给药浓度有一定关系,其中以中中组疗效最好。体现中药甘丹油外用的机理可能是通过抗炎消肿而改善湿疹的皮损,为临床治疗婴幼儿湿疹和亚急性、慢性湿疹提供理论基础。
[Abstract]:Objective to observe the clinical efficacy of Gandan oil in the treatment of infant eczema by observing the anti-inflammatory and antipruritic effect of Gandan oil on eczema model mice by experimental study in mice.The immunological effects of Gandan oil on IL-2NF- 伪 IFN- 纬 Ra and CD _ 4 CD _ 8 in mice blood were observed.Methods in the part of pharmacodynamics study on the animals, the mice were treated with 2% 4-dinitrochlorobenzene 24-DNCBs daily on the ear, back skin and eczema. 0.5% 4-aminopyridine 4-AP) was used to make the pruritus model, and the traditional Chinese medicine Gandan oil was used to treat the pruritus model.To observe the times of licking reaction, to compare the thickness and weight of the two ears, to compare the changes of inflammatory cells in the skin tissue of the ears, and to detect the content of the skin lesions in the back of the mice by immunohistochemical method.The clinical case study of 122 cases of infantile eczema were randomly divided into treatment group (61 cases) and control group (61 cases).The treatment group was treated with gandan oil and the control group with hydrocortisone butyrate ointment.The changes of the total score, the curative effect before and after treatment, and the morphologic features of rash were compared between the two groups.Results the therapeutic effect of Gandan oil on infant eczema was definite .2 the external application of Gandan oil had obvious anti-inflammatory effect on the skin lesions of mice model of eczema. The mechanism was to moisturize the skin and relieve itching and regulate the cytokines secreting in mice.Conclusion the results of the clinical case study showed that the control group was superior to the treatment group, but the treatment group was superior to the control group in terms of its recurrence rate and side effects.The results showed that Gandan oil was effective in treating infant eczema, and could be used as an ideal drug for treating infant eczema. The pharmacodynamics of mice showed that the model of auricle swelling was induced by external use of Gandan oil, which was an ideal drug for the treatment of infant eczema.All the models of eczema on the back had obvious inhibitory effect, which had anti-inflammatory and swelling effect, and had obvious inhibitory effect on the mice skin pruritus model induced by 2C4-DNCB, and its antipruritus effect was related to the concentration of drug administration, and the middle group had the best curative effect.The mechanism of external application of Gandan oil may be to improve the skin lesions of eczema through anti-inflammation and detumescence, which provides a theoretical basis for the clinical treatment of infantile eczema, subacute and chronic eczema.
【学位授予单位】：南京中医药大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R275.9

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