瘢痕灸预处理对原发性肝癌大鼠Wnt信号通路相关蛋白表达的影响

发布时间：2018-04-15 22:34

本文选题：瘢痕灸预处理 + 肝俞　；参考：《中华中医药学刊》2017年05期

【摘要】：目的:探讨瘢痕灸预处理肝俞及足三里穴对原发性肝癌大鼠Wnt信号传导通路相关蛋白β-连环蛋白(β-catenin)、分泌型卷曲相关蛋白(s FRP1)表达的影响。方法:48只Wistar雄性大鼠,随机分为对照组、模型组、预处理肝俞组、预处理足三里组,每组12只。预处理肝俞组和预处理足三里组均提前2周施行瘢痕灸后,与模型组一同采用腹腔注射二乙基亚硝胺(50 mg/kg)建立大鼠原发性肝癌(primary liver cancer,PLC)模型,记录各组大鼠体质量,造模结束后取大鼠肝脏,采用Western blot方法测定β-catenin,s FRP1表达量,HE染色观察大鼠肝脏组织形态学变化。结果:与对照组相比,模型组、预处理肝俞组和预处理足三里组的s FRP1表达量明显下降(P0.05),β-catenin的表达量明显升高(P0.05);与模型组比较,预处理肝俞组和预处理足三里组s FRP1表达量明显升高(P0.05),β-catenin的表达量显著降低(P0.05);与预处理肝俞组比较,预处理足三里组s FRP1表达量明显降低(P0.05),β-catenin的表达量升高(P0.05)。结论:瘢痕灸预处理肝俞和足三里对预防原发性肝癌均有良性作用,且肝俞穴效果优于足三里穴。
[Abstract]:Objective: to investigate the effect of scar moxibustion preconditioning at Ganshu and Zusanli points on the expression of 尾 -cateninine (尾 -cateninine), a secretory curl associated protein (FRP1), in rats with primary liver cancer (HCC).Methods Forty-eight male Wistar rats were randomly divided into control group, model group, Ganshu preconditioning group and Zusanli preconditioning group with 12 rats in each group.After preconditioning Ganshu group and Zusanli group were given scar moxibustion two weeks in advance, the primary liver cancer model was established by intraperitoneal injection of diethylnitrosamine 50 mg / kg together with the model group, and the body weight of each group was recorded.At the end of the model, the liver of rats was taken out. The expression of 尾 -cateninins FRP1 was determined by Western blot method and the histomorphology of the liver was observed by HE staining.Results: compared with the control group, the expression of s FRP1 and 尾 -catenin in the model group, Ganshu preconditioning group and Zusanli preconditioning group were significantly decreased, and the expression of 尾 -catenin was significantly increased in the model group, and compared with the model group, the expression of 尾 -catenin in the model group was significantly higher than that in the model group.The expression of s FRP1 and 尾 -catenin were significantly increased in Ganshu preconditioning group and Zusanli group, and the expression of 尾 -catenin was significantly decreased compared with that in Ganshu preconditioning group, the expression of s FRP1 in Zusanli preconditioning group was significantly lower than that in Ganshu preconditioning group, and the expression of 尾 -catenin was significantly increased in Zusanli preconditioning group.Conclusion: both Ganshu and Zusanli pretreated with scar moxibustion have benign effects on the prevention of primary liver cancer, and the effect of Ganshu point is better than that of Zusanli point.
【作者单位】：辽宁中医药大学针灸推拿学院;沈阳市中医院内科;辽宁中医药大学第一临床学院;
【基金】：辽宁省科学技术基金项目(2014020047)
【分类号】：R245

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