电针治疗对颞下颌关节紊乱综合症大鼠TNF-α 、IL-1β影响的研究

发布时间：2018-04-23 02:28

本文选题：电针治疗 + 颞下颌关节紊乱综合征　；参考：《广州中医药大学》2016年硕士论文

【摘要】：研究目的：颞下颌关节紊乱综合症(temporomandibular joint dysfunction syndrome,TMJDS),又称颞下颌功能紊乱病(temporomandibular disorders, TMD),是口腔科的常见疾病,临床症状较重,对患者的生活影响较大,会引起咀嚼功能和语言障碍；TMD的病程较长,反复难愈,给患者心理上蒙上一层厚厚的阴影,该疾病给患者带来精神方面和生活方面的负面影响较大。深入探讨颞下颌关节紊乱综合症的病因病机和发病机制,寻找有效的治疗方法,是现代医学研究的重要任务。本实验通过拔出大鼠右上颌磨牙,使大鼠长期偏侧咀嚼而造成颞下颌关节紊乱综合症实验动物模型,以炎性因子IL-1β TNF-α为观察指标,观察电针治疗对颞下颌关节紊乱综合征大鼠的血清和颞下颌关节及组织内炎性因子TNFα、IL-1β的影响。从细胞分子水平探讨电针疗法治疗颞下颌关节紊乱综合症的作用机理,为电针疗法治疗颞下颌关节紊乱综合症给予科学的理论支持。研究方法：SPF级SD大鼠共30只,雌雄各半,体重180-220g,实验分电针治疗组、空白对照组、模型组3组,每组10只。通过拔除大鼠右上颌磨牙,人为造成大鼠偏侧咀嚼,建立大鼠颞下颌关节紊乱综合征(TMD)模型。造模2周后进行电针治疗,针刺取穴为“下关”、“太阳”穴,导线连接双侧“太阳”和“下关”穴,治疗时间为两周,模型组和空白组大鼠每天抓握5分钟,抓握方式同电针组。持续2周治疗完成后,在不同组别的大鼠中采血并提取血清运用酶联免疫吸附反应(ELISA)法检测各组TNFα、IL-1β水平,处死大鼠后提取颞下颌关节及相关组织,研磨匀浆后取上清液运用酶联免疫吸附反应(ELISA)法检测各组TNFα、IL-1β水平,分析它们之间的关系,并运用统计学方法对比各组实验数据,采用SPSS19.0统计软件进行统计学处理。结果：模型组与空白组对照比较,模型组大鼠颞下颌关节内IL-1β、TNF-α水平大幅升高(p0.05)；与模型组对照比较,各电针组IL-1β、TNF-α含量明显降低(p0.05)。电针治疗对大鼠颞下颌关节内的炎性因子IL-1β、TNF-α的影响密切相关,差异有统计学意义(p0.05)。结论：1.电针能够有效降低大鼠血清中IL-1β、TNF-α等促炎因子的表达水平,从而提高血清抗炎因子的表达水平。2.电针治疗可以通过调节与颞下颌关节紊乱综合症有关的炎症反应,抑制相关炎症反应,控制颞下颌关节紊乱综合症的发作,进而抑制颞下颌关节紊乱综合症的病变过程。3.电针治疗可明显抑制模型大鼠颞下颌关节炎的指数,具有一定抗炎消肿镇痛作用,达到治疗的目的。
[Abstract]:Objective: temporomandibular joint dysfunction syndrome is a common disease in stomatology. TMD can cause masticatory function and language disorder, the course of TMD is longer, and it is difficult to heal repeatedly, and the patient is covered with a thick shadow on the psychology. The disease has a great negative effect on the mental and life aspects of the patients. It is an important task of modern medical research to explore the etiology, pathogenesis and pathogenesis of temporomandibular joint disorder syndrome and to find effective treatment methods. The experimental animal model of temporomandibular joint disorder syndrome was established by pulling out the right maxillary molars in rats, and the inflammatory factor IL-1 尾 TNF- 伪 was used as the observation index. To observe the effect of electroacupuncture on the inflammatory factor TNF 伪 and IL-1 尾 in serum, temporomandibular joint (TMJ) and temporomandibular joint (TMJ) in rats with temporomandibular disorder syndrome (TMJ). To explore the mechanism of electroacupuncture in the treatment of temporomandibular joint disorder syndrome from the cellular and molecular level, and to provide scientific theoretical support for electroacupuncture treatment of temporomandibular joint disorder syndrome. Methods Thirty Sprague-Dawley rats of SPF grade were divided into three groups: electroacupuncture group, blank control group and model group with 10 rats in each group. The rat model of temporomandibular joint disorder syndrome (TMJS) was established by pulling out the right maxillary molars and making partial mastication. After 2 weeks of model making, the rats were treated with electroacupuncture. The acupuncture points were "Xiaguan", "Sun" and "Sun" points. The treatment time was two weeks. The rats in the model group and blank group were clasped for 5 minutes every day. Grip mode is the same as electroacupuncture group. After 2 weeks of treatment, blood was collected from different groups of rats and serum was extracted to detect the level of TNF 伪 and IL-1 尾 by Elisa. The temporomandibular joint and related tissues were extracted after the rats were killed. After grinding homogenate, the supernatant was collected to detect the level of TNF 伪 and IL-1 尾 in each group by enzyme linked immunosorbent assay (Elisa), and the relationship between them was analyzed. The statistical method was used to compare the experimental data of each group and the statistical software SPSS19.0 was used to deal with them. Results: compared with the control group, the level of IL-1 尾 -TNF- 伪 in the temporomandibular joint in the model group was significantly higher than that in the control group, and the content of IL-1 尾 -TNF- 伪 in each electroacupuncture group was significantly lower than that in the model group. The effect of electroacupuncture on the inflammatory factor IL-1 尾 -TNF- 伪 in temporomandibular joint of rats was closely related, and the difference was statistically significant (P 0.05). Conclusion 1. Electroacupuncture can effectively reduce the expression of proinflammatory factors such as IL-1 尾 -TNF- 伪 in serum of rats, and thus increase the expression level of anti-inflammatory factors in serum. Electroacupuncture can control the attack of temporomandibular joint disorder syndrome by regulating the inflammatory response associated with temporomandibular joint disorder syndrome, and then inhibit the pathological process of temporomandibular joint disorder syndrome. Electroacupuncture can obviously inhibit the index of temporomandibular arthritis in model rats.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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