基因芯片筛选电针预处理心肌缺血再灌注大鼠细胞色素P450代谢通路相关差异表达基因

发布时间：2018-04-24 22:06

本文选题：基因芯片技术 + 电针预处理　；参考：《辽宁中医杂志》2017年04期

【摘要】：目的:利用基因芯片技术筛选电针预处理心肌缺血再灌注(MIR)大鼠心肌组织中细胞色素P450代谢通路相关差异表达的基因,寻找电针预处理对MIR大鼠产生保护作用的可能发生机制。方法:72只成年Wistar大鼠,雌雄各半,随机分为假手术组(Sham)、模型组(IR)、夹脊组(JJ)、内关组(NG)、阳陵泉组(YLQ)、曲池组(QC),每组12只。电针预处理组每天针刺30 min,连续针刺7 d。采用结扎左冠状动脉前降支的方法复制心肌缺血再灌注损伤模型。提取心肌组织总RNA并进行评估,根据Agilent One-Color Microarray-Based Gene Expression Analysis实验方案进行样品标记和芯片杂交,使用Agilent Feature Extraction、Gene Spring GX v12.1进行数据处理,使用标准的富集计算方法进行GO分析和Pathway分析。结果:电针预处理能够调控缺血再灌注大鼠的基因表达,其中夹脊穴组和内关穴组对细胞色素P450代谢通路相关基因影响显著,上调CYP450表氧化酶基因Cyp2b3、Cyp2c11、Cyp2b2、Cyp2s1,同时下调ω-羟化酶基因Cyp4a2、Cyp1a1、Cyp1b1,阳陵泉组和曲池组无显著性差异。结论:多个基因参与心肌缺血再灌注过程,涉及多条信号通路,电针夹脊穴和内关穴能够上调CYP2家族基因,下调CYP1和CYP4家族基因,从分子水平阐明了其对MIRI的保护作用机制。
[Abstract]:Aim: to screen the differentially expressed genes related to cytochrome P450 metabolic pathway in myocardial tissue of rats with electroacupuncture preconditioning myocardial ischemia reperfusion (MIR) by gene chip technique, and to find out the possible mechanism of protective effect of electroacupuncture preconditioning on MIR rats. Methods Seventy-two adult Wistar rats, half male and half female, were randomly divided into sham-operated group, model group, JJN group, Neiguan group, Yanglingquan group and Quchi group, 12 rats in each group. The electroacupuncture preconditioning group was treated with acupuncture for 30 minutes per day for 7 days. The model of myocardial ischemia reperfusion injury was established by ligating the anterior descending branch of left coronary artery. Total RNA of myocardial tissue was extracted and evaluated. According to the experimental scheme of Agilent One-Color Microarray-Based Gene Expression Analysis, sample labeling and chip hybridization were carried out, data were processed with Agilent Feature exactionator Gene Spring GX v12.1, go analysis and Pathway analysis were carried out using standard enrichment calculation method. Results: electroacupuncture preconditioning could regulate the gene expression of ischemia reperfusion rats, including Jiaji group and Neiguan point group, which had significant effects on the genes related to cytochrome P450 metabolic pathway. The gene Cyp2b3, Cyp2c11, Cyp2b2Cyp2s1, and the down-regulation of 蠅 -hydroxylase gene, Cyp4a2Cyp1a1 and Cyp1b1, were up-regulated, but there was no significant difference between Yanglingquan group and Quchi group in the up-regulation of Cyp2b3, Cyp2c11, Cyp2b2, Cyp2s1 and Cyp1b1b1. Conclusion: multiple genes are involved in the process of myocardial ischemia-reperfusion. Electroacupuncture at Jiaji point and Neiguan point can up-regulate CYP2 family genes and down-regulate CYP1 and CYP4 family genes. The mechanism of its protective effect on MIRI is elucidated at molecular level.
【作者单位】：山东中医药大学;山东中医药大学针灸推拿学院;山东中医药大学附属医院针灸科;
【基金】：国家自然科学基金(81373722) 山东省自然科学基金联合专项(ZR2014HL100)
【分类号】：R245

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