针灸对环磷酰胺化疗小鼠骨髓造血微环境中SDF-1及LFA-1影响的研究

发布时间：2018-04-26 10:22

本文选题：环磷酰胺 + 骨髓抑制　；参考：《河南中医药大学》2016年硕士论文

【摘要】：目的通过观察针灸对环磷酰胺(Cyclophosphamide,CTX)所致骨髓抑制模型小鼠骨髓组织中基质细胞衍生因子-l(SDF-1)及淋巴细胞相关抗原-1(LFA-1)含量的影响,从分子生物学水平揭示针灸修复和保护化疗后骨髓造血微环境的作用机理,从而为临床应用针灸保护化疗患者的造血功能,提升外周血白细胞提供分子生物学的实验室依据。方法将80只SPF级雄性昆明种小鼠按治疗天数不同依其体重随机分为3天组和5天组,每组再按其体重随机分成4组:空白组、模型组、针刺组、艾灸组;每亚组各10只。用CTX建立骨髓抑制模型。造模成功后,每天定时专人对针刺组、艾灸组小鼠“大椎”、“膈俞”、“肾俞”、“足三里”给予针刺、艾灸治疗,根据所分组不同,分别连续治疗3或5天。空白组、模型组每日陪同抓取固定,不治疗。各组小鼠每日定时专人尾静脉采血,镜检外周血白细胞计数;酶联免疫吸附试验检测各组小鼠骨髓细胞SDF-1和LFA-1含量,采用SPSS20.0 for Windows统计软件进行数据统计分析。结果1造模后,3天组和5天组接受CTX化疗的各组小鼠与空白组比白细胞数明显下降(P0.05),提示2组均造模成功。治疗3天后,模型组、针刺组、艾灸组白细胞数均低于空白组(P0.05)。治疗5天后,模型组白细胞数低于空白组(P0.05),而针刺组、艾灸组白细胞数均高于模型组和空白组(P0.05)。模型组造模前白细胞最高,然后呈下降趋势,在治疗3天后白细胞降至最低,然后呈上升趋势。针刺组、艾灸组造模前白细胞最高,然后呈下降趋势,在治疗第2天后白细胞最低,然后呈上升趋势。2造模后,接受CTX化疗的各组小鼠SDF-1含量与空白组比明显下降(P0.05),提示CTX抑制骨髓趋化因子SDF-1的表达。治疗3天与5天后SDF-1含量变化规律非常相近。与空白组比,模型组、针刺组、艾灸组SDF-1含量有所下降(P0.05)。但针刺组、艾灸组SDF-1的含量明显高于模型组(P0.05),针刺组与艾灸组比,艾灸组略高于针刺组但差异无统计学意义(P0.05)。3造模后,接受CTX化疗的各组小鼠LFA-1含量与空白组比明显升高(P0.05),提示CTX促进骨髓黏附分子LFA-1的表达。治疗3天后与治疗5天后LFA-1含量变化规律大致相同:与空白组比,模型组、针刺组、艾灸组LFA-1含量有所上升(P0.05),但针刺组、艾灸组LFA-1含量明显低于模型组(P0.05)。治疗3天后与治疗5天后两者的差别在于:治疗3天后针刺组与艾灸组比,艾灸组LFA-1略低于针刺组但差异无统计学意义(P0.05),治疗5天后艾灸组LFA-1低于针刺组且差异有统计学意义(P0.05)。4治疗天数不同对SDF-1、LFA-1含量的影响。针刺3天或5天对小鼠骨髓中SDF-1、LFA-1含量的影响,差异均无统计学意义(P0.05)。艾灸3天或5天对小鼠骨髓中SDF-1表达含量的差异亦无统计学意义(P0.05),但艾灸5天组小鼠骨髓中LFA-1含量低于艾灸3天组小鼠骨髓中LFA-1含量(P0.05)。结论1针灸治疗可以拮抗CTX化疗所致的骨髓抑制,改善骨髓造血功能,提高外周血白细胞计数。2针刺和艾灸可以通过上调CTX化疗小鼠骨髓中趋化因子SDF-1的表达,增强造血干/祖细胞的趋化作用,调节骨髓中干/祖细胞的迁移,促进早期血细胞的生成和增殖,起到修复化疗后骨髓造血微环境损伤的作用。3针刺和艾灸可以通过下调CTX化疗小鼠骨髓中黏附因子LFA-1的表达,降低造血干细胞与骨髓基质的牢固黏附,促进造血祖细胞增殖和分化,保护骨髓造血微环境,维护化疗后骨髓造血功能。4针刺和艾灸相比,艾灸更有利于通过下调CTX化疗小鼠骨髓中黏附因子LFA-1含量的途径降低造血祖细胞与基质的黏附,拮抗抑制性调节因子对造血祖细胞的损伤。
[Abstract]:Objective To observe the effect of acupuncture on the content of matrix cell derived factor -l (SDF-1) and lymphocyte associated antigen -1 (LFA-1) in bone marrow tissue of mice induced by Cyclophosphamide (CTX), and to reveal the mechanism of the action of acupuncture and moxibustion and protecting hematopoietic microenvironment after chemotherapy and chemotherapy. Clinical use of acupuncture and moxibustion to protect the hematopoietic function of chemotherapy patients and improve the molecular biology of peripheral blood leukocytes. Methods 80 SPF grade male Kunming mice were randomly divided into 3 days and 5 days according to their weight, and each group was divided into 4 groups according to their weight: blank group, model group, acupuncture group and moxibustion group. 10 rats in each subgroup were used to establish a bone marrow suppression model with CTX. After the success of the model, every day was given to the acupuncture group, the moxibustion group "Da Zhui", "Zushu", "Shenshu", "Zusanli", and moxibustion treatment. According to the different groups, the group was treated for 3 or 5 days. The blank group, the model group was accompanied daily and fixed, and the treatment was not treated. Group mice were given blood for collecting blood by tail vein every day, the white blood cell count of peripheral blood was examined by microscope, the content of SDF-1 and LFA-1 in bone marrow cells of each group were detected by enzyme linked immunosorbent assay, and the data were analyzed by SPSS20.0 for Windows software. The results of 1 models were compared with white blood cells in the 3 day group and 5 day group which received CTX chemotherapy. After 3 days, the number of white blood cells in model group, acupuncture group and moxibustion group were lower than that of blank group (P0.05). The number of white blood cells in model group was lower than that of blank group (P0.05) after 5 days treatment. The number of white blood cells in acupuncture group and moxibustion group were higher than that of model group and blank group (P0.05). The highest white blood cells in model group were before model building. After 3 days of treatment, the white blood cells decreased to the lowest, and then showed an upward trend. In the acupuncture group, the moxibustion group had the highest white blood cells before making the model, and then showed a downward trend. After second days of treatment, the white blood cells were the lowest, and then the.2 model was on the rise. The SDF-1 content in each group of rats received CTX chemotherapy was significantly lower than that in the blank group (P0.05), suggesting CTX Inhibition of the expression of bone marrow chemotactic factor SDF-1. The changes of SDF-1 content in 3 days and 5 days were very similar. Compared with the blank group, the content of SDF-1 in the model group, the acupuncture group and the moxibustion group decreased (P0.05). However, the content of SDF-1 in the acupuncture group and moxibustion group was significantly higher than that of the model group (P0.05), the ratio of the acupuncture group to the moxibustion group and the moxibustion group were slightly higher than the acupuncture group, but the difference was slightly higher than that in the acupuncture group. Without statistical significance (P0.05).3, the content of LFA-1 in each group of mice received CTX chemotherapy was significantly higher than that in the blank group (P0.05), suggesting that CTX promoted the expression of LFA-1 of bone marrow adhesion molecule. The changes of LFA-1 content in 3 days after treatment and 5 days after treatment were roughly the same as that in the blank group, and the LFA-1 content in the model group, the acupuncture group and the moxibustion group increased (P0.0). 5), but in the acupuncture group, the content of LFA-1 in the moxibustion group was significantly lower than that of the model group (P0.05). The difference between the 3 days after treatment and the treatment for 5 days was that the LFA-1 of the acupuncture group and the moxibustion group was slightly lower than the acupuncture group for 3 days, but the difference was not statistically significant (P0.05), and the LFA-1 in the moxibustion group was lower than the acupuncture group for 5 days, and the difference was statistically significant (P0.05).4. The effect of different treatment days on the content of SDF-1 and LFA-1. The effect of 3 days or 5 days on the content of SDF-1 and LFA-1 in the bone marrow of mice was not statistically significant (P0.05). The difference of SDF-1 expression in the bone marrow of mice was not statistically significant (P0.05) at 3 days or 5 days, but the content of LFA-1 in the bone marrow of the 5 day moxibustion group was lower than that of the 3 day moxibustion group. Conclusion the content of LFA-1 in rat bone marrow (P0.05). Conclusion 1 acupuncture and moxibustion therapy can antagonize the bone marrow suppression caused by CTX chemotherapy, improve the hematopoietic function of bone marrow, improve the peripheral blood leukocyte count,.2 acupuncture and moxibustion can increase the expression of chemokine SDF-1 in the bone marrow of the mice with CTX chemotherapy, enhance the chemotactic effect of the blood stem / progenitor cells and regulate the stem / progenitor in the bone marrow. Cell migration promotes the formation and proliferation of early blood cells, and plays the role of repairing hematopoietic microenvironment injury after chemotherapy.3 acupuncture and moxibustion can reduce the expression of adhesion factor LFA-1 in the bone marrow of mice with CTX chemotherapy, reduce the strong adhesion of hematopoietic stem cells and bone marrow stroma, promote the proliferation and differentiation of hematopoietic progenitor cells and protect the bone. Compared with.4 acupuncture and moxibustion, moxibustion is more beneficial to reduce the adhesion of the hematopoietic progenitor cells to the matrix by lowering the content of the adhesion factor LFA-1 in the bone marrow of the mice treated with CTX, and antagonize the damage of the inhibitory regulatory factors to the hematopoietic progenitor cells.

【学位授予单位】：河南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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