电针对缓解期偏头痛大鼠脑5-羟色胺1F受体mRNA及蛋白表达的影响

发布时间：2018-04-26 22:36

本文选题：偏头痛 + -羟色胺受体　；参考：《中医杂志》2017年02期

【摘要】：目的探究电针治疗缓解期偏头痛的可能作用机制及腧穴配伍的效应。方法将30只SD大鼠随机分为对照组、模型组、单穴组、配穴组、假穴组,每组6只。除对照组外其余各组大鼠颅顶钻孔植入电极手术后电刺激硬脑膜建立缓解期偏头痛模型。单穴组电针双侧风池穴,配穴组电针双侧风池穴加双侧阳陵泉穴,假穴组取大鼠腰部非穴位点,于造模后第2、4、6天实行清醒状态下电针干预,并测定大鼠足底和面部痛阈值,第7天检测大鼠脑三叉神经节、三叉神经脊束核尾核、中缝大核中5-羟色胺1F(5-HT1F)受体mRNA及蛋白表达。结果第2、4、6天模型组大鼠面部、足底痛阈值均明显低于同时间对照组(P0.05);单穴组和配穴组面部、足底痛阈值均高于同时间模型组(P0.05);单穴组面部、足底痛阈值低于同时间配穴组而高于同时间假穴组(P0.05)。模型组大鼠脑三叉神经节、三叉神经脊束核尾核、中缝大核中5-HT1F受体mRNA及蛋白表达明显低于对照组(P0.05);单穴组和配穴组各部位5-HT1F受体mRNA及蛋白表达明显高于模型组,并且配穴组高于单穴组(P0.05)。结论电针治疗缓解期偏头痛机制可能与5-HT1F受体激活作用有关,且远近配穴疗效优于单穴治疗。
[Abstract]:Objective to explore the possible mechanism of electroacupuncture in the treatment of migraine in remission period and the effect of acupoint compatibility. Methods Thirty Sprague-Dawley rats were randomly divided into control group, model group, single acupoint group, acupoint matching group and pseudo acupoint group, with 6 rats in each group. The model of migraine in remission stage was established by electrical stimulation of dura mater of rats except the control group. In the single point group, the bilateral Fengchi points were electroacupuncture, the two sides Fengchi points were combined with the bilateral Yanglingquan points in the combined acupoint group, and the lumbar non-acupoint points were taken in the sham point group, and the electroacupuncture intervention was carried out in the conscious state on the 2nd 4th day after the establishment of the model, and the threshold of plantar and facial pain in the rats was measured. On the 7th day, the expression of 5-hydroxytryptamine (5-HT) receptor mRNA and protein in trigeminal ganglion, caudate nucleus of spinal tract of trigeminal nerve and nucleus raphe Magnus were detected. Results the threshold of plantar pain in the model group was significantly lower than that in the control group at the same time (P 0.05), the threshold of plantar pain in the single point group and the matching point group was higher than that in the model group at the same time (P 0.05), the facial pain threshold in the single point group was higher than that in the control group. The threshold of plantar pain was lower than that of acupoint allocation group at the same time, but higher than that of pseudo-acupoint group at the same time (P 0.05). The expression of 5-HT1F receptor mRNA and protein in trigeminal ganglion, caudal nucleus of trigeminal nerve and nucleus raphe Magnus in model group was significantly lower than that in control group (P0.05A), and the expression of mRNA and protein of 5-HT1F receptor in single point group and point matching group was significantly higher than that in model group. And the point matching group was higher than the single point group (P 0.05). Conclusion the mechanism of electroacupuncture in the treatment of migraine in remission period may be related to the activation of 5-HT1F receptor, and the therapeutic effect of far and near matching points is better than that of single point therapy.
【作者单位】：首都医科大学附属北京中医医院;安徽中医药大学第一附属医院;

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