逆灸对力竭运动大鼠心肌组织中AMPK、mTOR的影响

发布时间：2018-04-27 03:16

本文选题：心肌损伤 + 逆灸　；参考：《中国针灸》2017年05期

【摘要】：目的:观测逆灸对力竭运动大鼠心肌组织中一磷酸腺苷活化的蛋白激酶(AMPK)、哺乳动物雷帕霉素靶蛋白(mTOR)的影响,探讨逆灸抗力竭运动心肌损伤的作用机制。方法:将18只雄性SD大鼠随机分为空白组、力竭组和逆灸组,每组6只。空白组大鼠每天被操作者用手轻按于操作台上,套上特制鼠套,保持5min,不进行任何干预;力竭组大鼠前10d不做处理,逆灸组取"足三里""关元"进行干预,每穴每日灸5壮,连续10d,第11d力竭组和逆灸组大鼠力竭游泳后取材,并记录两组大鼠达到力竭所用时间。采用HE染色观察各组大鼠心肌组织炎性反应;比色法检测血清中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;免疫抑制法检测血清中肌酸激酶同工酶(CK-MB)活性;Elisa检测血清中肌钙蛋白T(cTnT)含量;Western blot测定心肌组织中一磷酸腺苷活化的蛋白激酶α2(AMPKα2)、哺乳动物雷帕霉素靶蛋白(mTOR)表达。结果:逆灸组较力竭组到力竭状态所需时间延长[(4 831.17±689.88)s vs(3 509.50±1 232.49)s,P0.05],心肌组织结构清楚,炎细胞浸润减轻;与空白组比较,力竭组血清LDH、CK-MB、cTnT升高(均P0.05);MDA含量升高,SOD活性下降(均P0.05);心肌组织AMPKα2表达升高、mTOR下降(均P0.05)。与力竭组比较,逆灸组血清LDH、CK-MB、cTnT下降(均P0.05);MDA含量下降,SOD活性升高(均P0.05);心肌组织AMPKα2表达升高、mTOR下降(均P0.05);AMPKα2与mTOR呈负相关(r=-0.764,P0.05)。结论:逆灸可能通过调控AMPK/mTOR表达诱导相应的信号级联反应对抗一次性力竭运动大鼠心肌组织损伤。
[Abstract]:Objective: to observe the effect of reverse moxibustion on adenosine monophosphate activated protein kinase AMPKO and mammalian rapamycin target protein mTORin in myocardial tissue of exhaustive exercise rats, and to explore the mechanism of anti-exhaustive exercise myocardial injury by reverse moxibustion. Methods: eighteen male Sprague-Dawley rats were randomly divided into blank group, exhaustive group and reverse moxibustion group with 6 rats in each group. The rats in the blank group were pressed lightly by the operator on the operating platform with their hands every day, the rats in the exhaustive group were not treated for the first 10 days, and the rats in the reverse moxibustion group were treated with "Zusanli" and "Guanyuan" at 5 strong points per day for 5 minutes, and the rats in the exhaustive group were not treated for 5 minutes, and the rats in the reverse moxibustion group were treated with "Zusanli" and "Guan Yuan". The rats in the exhaustion group and the reverse moxibustion group were taken after exhaustive swimming for 10 consecutive days, and the time to reach exhaustion was recorded in the two groups. The myocardial inflammatory reaction was observed by HE staining, the activity of LDH, SOD and the content of malondialdehyde (MDA) in serum were detected by colorimetric method. The activity of creatine kinase isoenzyme (CK-MBB) in serum was determined by immunosuppressive assay. The content of troponin TnT in serum was determined by Western blot. The expression of adenosine monophosphate activated protein kinase 伪 2(AMPK 伪 2 and mammalian rapamycin target protein mTORwere detected by Western blot. Results: the time from exhaustion to exhaustion was prolonged in the reverse moxibustion group [4 831.17 卤689.88 s vs(3 509.50 卤1 232.49 s P 0.05], the myocardial tissue structure was clear and the inflammatory cell infiltration was reduced, compared with the blank group. In the exhaustive group, the level of serum AMPK 伪 2 and the expression of AMPK 伪 2 were increased (all P 0.05) and the expression of AMPK 伪 2 was decreased (all P 0.05), while the activity of sod was decreased (P 0.05), and the expression of AMPK 伪 2 was increased (P 0.05). Compared with the exhaustive group, the serum level of AMPK 伪 2 in the moxibustion group was significantly lower than that in the exhaustive group (P 0.05), the activity of AMPK 伪 2 was increased (P 0.05), and the expression of AMPK 伪 2 in myocardium was significantly lower than that in the control group (P 0.05), and there was a negative correlation between AMPK 伪 2 and mTOR (r -0.764P 0.05). Conclusion: moxibustion may induce signal cascade reaction by regulating the expression of AMPK/mTOR against myocardial tissue injury in rats with exhaustive exercise.
【作者单位】：南京中医药大学第二临床学院;
【基金】：国家自然科学基金面上项目:81574062,81674063 江苏省高校自然科学研究重大项目:16KJA 360003 江苏省普通高校研究生科研创新计划资助项目:KYLX 15-1004
【分类号】：R245

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