出血性络风内动证小鼠模型的构建

发布时间：2018-04-29 16:43

本文选题：出血性络风内动证 + 动物模型　；参考：《中医杂志》2017年11期

【摘要】：目的建立符合临床、具有易行性与广泛适用性的出血性络风内动证动物模型。方法 Apo E(-/-)基因敲除小鼠高脂饲料喂养并于12周龄行右侧颈总动脉套管术,术后随机分为对照组、应激组、Compound组、应激+Compound组。术后4周后对所有小鼠腹腔注射脂多糖1mg/kg,每周2次,共8周;术后8周应激组、应激+Compound组给予限制性精神刺激,每天4h,共4周;术后10周Compound组、应激+Compound组小鼠腹腔注射Compound 48/80 0.5 mg/kg,隔日1次,共4次。术后12周进行血脂检测和斑块病理形态学检测,计算斑块面积、血管管腔狭窄率,比较斑块内脂质、胶原含量、平滑肌细胞和单核/巨噬细胞含量并计算斑块易损指数。结果各组小鼠血脂水平差异均无统计学意义(P0.05)。与对照组比较,Coumpound组斑块内出血情况明显升高,应激+Compound组总斑块病理形态情况差异有统计学意义(P0.05)。各组斑块面积、血管管腔狭窄率及斑块中脂质、平滑肌细胞含量比较差异均无统计学意义(P0.05),而应激+Compound组和Coumpound组的单核/巨噬细胞含量则较对照组明显升高(P0.05)。应激+Compound组斑块内胶原含量较其余各组减少,而易损指数较其余各组升高(P0.05)。结论采用脂多糖+应激+Compound 48/80三种因素联合刺激进行出血性络风内动证小鼠模型构建具有较高的斑块易损性和斑块内出血率,可作为出血性络风内动证动物模型的最佳造模方法。
[Abstract]:Objective to establish an animal model of hemorrhagic collateral wind syndrome with clinical, easy and wide applicability. Methods Apo E- / R) knockout mice were fed with high fat diet and were treated with right common carotid artery cannula at the age of 12 weeks. After the operation, they were randomly divided into control group, stress group and stress Compound group. Four weeks after operation, all mice were intraperitoneally injected with lipopolysaccharide 1 mg / kg, twice a week for 8 weeks; in the stress group, the stress Compound group was given restricted mental stimulation for 4 weeks, 4 hours per day, and 10 weeks after the operation, in the Compound group, Mice in stress Compound group were injected Compound 48 / 80 0.5 mg / kg intraperitoneally once every other day for 4 times. The plaque area, vascular stenosis rate, lipid content, collagen content, smooth muscle cell and mononuclear / macrophage content were compared and plaque vulnerability index was calculated 12 weeks after operation. Results there was no significant difference in blood lipid level in each group (P 0.05). Compared with the control group, the plaque hemorrhage in the Coumpound group was significantly increased, and the pathological morphology of the total plaque in the stress Compound group was significantly higher than that in the control group (P 0.05). There was no significant difference in plaque area, vascular lumen stenosis rate, lipid content and smooth muscle cell content in each group, but the monocyte / macrophage content in stress Compound group and Coumpound group was significantly higher than that in control group. The content of collagen in plaque in stress Compound group was lower than that in other groups, while the vulnerability index was higher than that in other groups (P 0.05). Conclusion the model of hemorrhagic collateral wind induced by lipopolysaccharide stress (Compound 48 / 80) combined with stimulation has high plaque vulnerability and intraplaque bleeding rate. It can be used as the best modeling method for the animal model of hemorrhagic collateral wind internal movement syndrome.
【作者单位】：北京中医药大学东直门医院;
【基金】：国家自然科学基金(81273913) 北京中医药大学重点学科开放课题(2013-ZDXKKF-27)
【分类号】：R259;R-332

【相似文献】