肿节风和马蔺子素对大鼠放射性肺损伤的防护研究

发布时间：2018-05-01 17:42

本文选题：放射性肺损伤 + 肿节风　；参考：《广西医科大学》2016年硕士论文

【摘要】：目的：观察肿节风和马蔺子素对SD大鼠放射性肺损伤的防护作用。方法：采用随机数字表法将120只SD大鼠完全随机分为正常组(A组)、单纯照射组(B组)、肿节风照射组(C组)、马蔺子素照射组(D组),每组30只。正常组(A组)、单纯照射组(B组)灌服等体积的生理盐水；肿节风照射组(C组)灌服肿节风溶液(5g·kg-1·d-1),马蔺子素照射组(D组)灌服马蔺子素的溶液(31mg·kg-1·d-1),照射前连续灌服1周, 照射后继续灌服至肺取出。单纯照射组(B组)、肿节风照射组(C组)、马蔺子素照射组(D组)均采用60Co Y治疗机单次20Gy右全肺照射,建立大鼠放射性肺损伤模型,正常组(A组)不予照射。照射后第1、4、8、12、24周取大鼠肺组织苏木素伊红染色(HE染色)、马松三色染色(Masson染色)进行组织病理学观察；紫外分光光度计检测肺组织中羟脯氨酸含量；酶联免疫吸附测定法(ELISA)检测血清TNF-α、IL-1β和TGF-β1的浓度；免疫组化法检测肺组织中肿瘤坏死因子α(TNF-α)和转化生长因子β1(TGF-β1)的表达。结果：正常组(A组)在各个时间点肺泡结构完整,无炎性细胞渗出和水肿。单纯照射组(B组)在照射后1、4、8周出现淋巴细胞及中性粒细胞、巨噬细胞等炎细胞浸润灶,肺泡毛细血管普遍扩张、肺间质充血、水肿,肺泡壁、肺泡间隔逐渐增厚；12、24周,肺泡壁重度增厚,肺泡腔明显变小甚至缺失,支气管、血管、肺泡间隔有大量成纤维细胞增生形成纤维化。C组和D组在1、4、8、12周相同时间点也出现上述炎性细胞浸润灶等病理变化,但较同时期B组轻；24周,肺泡壁重度增厚,D组较B组纤维化程度轻,但C组与B组相比差别不大。血清TNF-α表达水平：在照射后1、4、8周,单纯照射组(B组)值均明显高于C组、D组,差异有统计学意义(P0.05)；而12、24周,与D组比较,差异有统计学意义(P0.05),但与C组比较,差异均无统计学意义(P0.05)。IL-1β表达水平：照射后1、4、8周,单纯照射组(B组)值均明显高于C组、D组,差异有统计学意义(P0.05)；而12、24周,与D组比较差异有统计学意义(P0.05),但与C组比较差异均无统计学意义(P0.05)。血清TGF-β1、肺组织羟脯氨酸含量表达水平：在照射后4、8周,单纯照射组(B组)值均明显高于C组、D组,差异有统计学意义(P0.05)；而12、24周,与D组比较,差异有统计学意义(P0.05),但与C组比较,差异均无统计学意义(P0.05)。肺组织TNF-a的表达：在照射4、8、12周,单纯照射组(B组)的表达均明显高于C组、D组,差异有统计学意义(P0.05)；而24周,与D组比较,差异有统计学意义(P0.05),但与C组比较,差异均无统计学意义(P0.05)。肺组织TGF-β1的表达：在照射后4、8、12周,单纯照射组(B组)的表达均明显高于C组、D组,差异有统计学意义(P0.05)；而24周,与D组比较,差异有统计学意义(P0.05),但与C组比较,差异均无统计学意义(P0.05)。结论：肿节风、马蔺子素均能降低早期放射性肺损伤大鼠血清中的TNF-α、IL-1β、TGF-β1含量及肺组织羟脯氨酸、TNF-α、TGF-β1的表达,对早放射性肺损伤具有防护作用,二者无差别。马蔺子素,能减轻放射性肺炎的同时、也能减轻晚期放射性肺纤维化。肿节风能减轻放射性肺炎,但对减轻晚期放射性肺纤维化作用不明显。
[Abstract]:Objective: To observe the protective effect of gal and iris on the radiation lung injury of SD rats. Methods: 120 SD rats were randomly divided into normal group (group A), simple irradiation group (group B), group C (group C), group D (group D), normal group (group A), simple irradiation group (B group). Isomer saline (group C) group (group C) administered orisomal solution (5g. Kg-1. D-1), iris irradiate group (group D) to fill in the solution of iris (31mg. Kg-1. D-1), before irradiation for 1 weeks, and continued to be taken to the lung after irradiation. The 60Co Y treatment machine single 20Gy right whole lung irradiation was used to establish a rat model of radionuclide lung injury. The normal group (group A) was not irradiated. After 1,4,8,12,24, the rat lung tissue was stained with hematoxylin (HE), and Masson staining (Masson staining) was used to observe the tissue disease. The ultraviolet spectrophotometer was used to detect the hydroxyproline in the lung tissue. The concentration of TNF- alpha, IL-1 beta and TGF- beta 1 in serum was detected by enzyme linked immunosorbent assay (ELISA); the expression of tumor necrosis factor alpha (TNF- alpha) and transforming growth factor beta 1 (TGF- beta 1) in lung tissue was detected by immunohistochemistry. Results: the normal group (group A) had complete alveolar structure at every time point, without inflammatory cell exudation and edema. In group B, lymphocytes and neutrophils, macrophages and other inflammatory cells infiltrated, alveolar capillaries expanded generally, pulmonary alveolar hyperemia, edema, alveolar wall, and alveolar septum gradually thickened; 12,24 weeks, the alveolar walls were thickened, alveolar cavity became smaller or even missing, bronchi, blood vessels, and alveolar septum were large The fibroblast proliferation and the formation of fibrotic.C group and D group also appeared in the same time point at the same time of 1,4,8,12. But it was lighter than the same period B group. 24 weeks, the alveolar wall thickened and the D group was lighter than the B group, but the C group was not very different from the B group. The serum TNF- alpha expression level: in 1,4,8 weeks after irradiation, simple The value of the group (group B) was significantly higher than that of the group C, and the difference in the group D was statistically significant (P0.05), while the difference was statistically significant (P0.05) compared with the D group (P0.05), but the difference was not statistically significant (P0.05) in the group of C (P0.05).IL-1 beta: the value of the simple irradiation group (B group) was significantly higher than that in the C group, and the difference was statistically significant. (P0.05), and 12,24 weeks, compared with the D group, there was significant difference (P0.05), but there was no significant difference between the C group and the C group (P0.05). The serum TGF- beta 1, the level of hydroxyproline content in the lung tissue was significantly higher than the C group and D group (P0.05) in 4,8 weeks after irradiation, and the difference was statistically significant (P0.05). The difference was statistically significant (P0.05), but compared with the C group, the difference was not statistically significant (P0.05). The expression of TNF-a in the lung tissue was significantly higher than that of the C group (B group) at 4,8,12 weeks, and the difference was statistically significant (P0.05) in the D group (P0.05), but the difference was statistically significant (P0.05) compared with the D group (P0.05), but compared with the C group, the difference was worse than that of the C group. There was no statistically significant difference (P0.05). Expression of TGF- beta 1 in lung tissue: at 4,8,12 weeks after irradiation, the expression of pure irradiation group (group B) was significantly higher than that in group C and group D (P0.05), but the difference was statistically significant (P0.05) compared with that of D group (P0.05), but the difference was not statistically significant (P0.05) compared with that of the C group (P0.05). Conclusion: swelling wind, The contents of TNF- - alpha, IL-1 beta, TGF- beta 1 and the expression of hydroxyproline, TNF- A and TGF- beta 1 in lung tissue of early radioactive lung injury rats can protect the early radioactive lung injury, and there is no difference between the two. It can reduce radiation pneumonitis, but it has no obvious effect on reducing late radiation pulmonary fibrosis.

【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R273

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