基于内皮损伤学说的冠心病中药干预研究

发布时间：2018-05-03 11:07

本文选题：冠心病 + 内皮损伤　；参考：《辽宁中医药大学》2017年硕士论文

【摘要】：目的:本研究依据“内皮损伤学说”,通过血清药理学实验,探究中药对内皮细胞和级联反应细胞干扰素的影响,为科研与临床验证中药治疗冠心病疗效提供候选检测方法。方法:1选取健康受试者10例,临床剂量静点舒血宁注射液,进行安全性临床观察,同时制备含药血清。2制作内皮细胞PAF损伤模型,以空白和含药血清培养细胞,观察细胞形态与增殖,以Elisa试剂盒检测细胞上清分泌物中NO、VE-Ca、ICAM-1、VCAM-1浓度,观察舒血宁对细胞及级联反应细胞干扰因素的影响,研究其对冠心病内皮损伤的干预作用。结果:1舒血宁注射液临床试验观察,受试者前后生命体征、肝肾功、血常规、凝血五项中,体温、淋巴细胞、中性粒细胞P值0.05,考虑为样本量过少所致;余项P值均0.05,表明用药前后无明显变化。不良反应1例,1级轻度,可能与试验药物无关。2造模:当以浓度为25umol/L的PAF作用4h后,细胞存活率平均值为47.45±6.95,细胞损伤率近50%,考虑为理想造模条件。细胞增殖随PAF浓度的升高、诱导时间的延长而受到抑制程度也加重,PAF诱导细胞损伤。3与模型对照组相比,含药血清组细胞形态生长状态良好,增殖72.9%±4.87%,NO浓度下降,VE-Ca、ICAM-1、VCAM-1浓度升高,与空白对照组相近,与模型对照组存在明显差异。结论1舒血宁注射液能够通过改善细胞形态,促进细胞增殖,调节细胞分泌功能,对内皮细胞和细胞级联反应干扰素产生影响。2以人体血清及细胞实验为依托的中药治疗冠心病疗效的候选检测方法具有可行性。
[Abstract]:Objective: To investigate the effect of Chinese medicine on the interferon in endothelial cells and cascade reaction cells through the theory of "endothelial injury", and to provide a candidate detection method for the therapeutic effect of Chinese medicine in the treatment of coronary heart disease by scientific research and clinical validation. 1 the clinical dose of Shuxuening injection was carried out in 10 healthy subjects. The PAF damage model of endothelial cells was prepared by the preparation of.2 containing serum, and the cell morphology and proliferation were observed with blank and drug containing serum. The concentration of NO, VE-Ca, ICAM-1 and VCAM-1 in the secretions of cell supernatant was detected by Elisa kit, and the effects of Shuxuening on the cell and cascade reaction cells were observed. The interventional effect of endothelium injury in coronary heart disease. Results: 1 the clinical trial of Shuxuening injection was observed in the clinical trial. In the subjects, the body temperature, the liver and kidney work, the blood routine and the coagulation five, the body temperature, the lymphocyte and the P value of neutrophils were 0.05, and the value of the remainder P was 0.05, indicating that there were no obvious changes before and after the drug use, 1 cases of adverse reactions and 1 light weight. Degree, may be irrelevant to the test drug.2 model: when the concentration of 25umol/L PAF action 4h, the average cell survival rate is 47.45 + 6.95, the cell damage rate is nearly 50%, considered as the ideal modeling condition. Cell proliferation with the increase of PAF concentration, the prolongation of induction time and the increase of inhibition range, PAF induced cell injury.3 and model control group In contrast, the cell morphologic growth of the serum containing drug group was 72.9% + 4.87%, the concentration of NO decreased, the concentration of VE-Ca, ICAM-1 and VCAM-1 increased, which was similar to that of the blank control group, and there was significant difference between the control group and the model control group. Conclusion 1 Shuxuening Injection can improve the cell morphology, promote the cell proliferation, regulate the cell secretion function, and the endothelial cells and the endothelial cells. Cell cascade reaction interferon production affects.2. It is feasible to detect human coronary heart disease with Chinese medicine based on human serum and cell experiments.

【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R259

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