维药青香茅中芹菜素-7-O-β-D-吡喃葡萄糖苷含量测定

发布时间：2018-05-04 00:18

本文选题：维药青香茅 + 芹菜素--O-β-D-吡喃葡萄糖苷　；参考：《中国医院药学杂志》2017年17期

【摘要】：目的:测定维药青香茅中芹菜素-7-O-β-D-吡喃葡萄糖苷含量。方法:采用高效液相色谱法,使用SinoChrom C18色谱柱(5μm,250 mm×4.6 mm),以甲醇-0.2%磷酸水溶液(41∶59)为流动相,检测波长324 nm。结果:芹菜素-7-O-β-D-吡喃葡萄糖苷在5.25~52.50μg·mL~(-1)范围内与峰面积呈良好的线性关系(r=0.999 9),方法平均回收率为:97.29%(RSD为1.23%),平均含量为57.912 1μg·g~(-1)。结论:本方法简便,准确,重复性好,可用测定维药青香茅中芹菜素-7-O-β-D-吡喃葡萄糖苷含量。
[Abstract]:Objective: to determine the content of apigenin-7-O- 尾-D-pyranoside in citronella citronella. Methods: high performance liquid chromatography (HPLC) was used with SinoChrom C18 column (250mm 脳 4.6mm). The mobile phase was methanol-0.2% phosphoric acid aqueous solution (41: 59). The detection wavelength was 324nm. Results: there was a good linear relationship between apigenin and peak area in the range of 5.25 ~ 52.50 渭 g / mL ~ (-1) for apigenin-7-O- 尾 -D- glucopyranoside. The average recovery of the method was 1. 23m ~ (-1) and the average content was 57.912 渭 g / g ~ (-1). Conclusion: this method is simple, accurate and reproducible, and can be used to determine the content of apigenin-7-O- 尾-D-pyranoside.
【作者单位】：新疆华世丹药业股份有限公司;新疆医科大学附属中医医院;新疆医科大学中医学院;
【基金】：新疆维吾尔自治区科技援疆项目(201491173)
【分类号】：R29

【相似文献】