电针预处理治疗布比卡因中毒大鼠伴膀胱排尿功能障碍的效果及作用机制

发布时间：2018-05-05 06:34

本文选题：电针预处理 + 布比卡因中毒　；参考：《毒理学杂志》2017年03期

【摘要】：目的探讨电针干预对布比卡因排尿功能障碍的影响及相关作用机制。方法选取6周龄60只清洁级SD雄性大鼠分为对照组、模型组和预处理组,每组各20只。模型组和预处理组均经腰麻注入建立布比卡因中毒大鼠模型。建模前模型组大鼠接受电针预处理。测定和记录各组大鼠膀胱测压参数。免疫染色组化法测定和比较各组大鼠逼尿肌细胞凋亡率和细胞bax、bcl-2表达,Western blot法测定和比较各组大鼠脊髓BDNF、Trk B和P2X3蛋白表达。结果对照组和预处理组大鼠单日排尿量、残余尿量和膀胱容量均显著高于模型组大鼠(P0.05),膀胱内压均显著低于模型组大鼠(P0.05)。对照组和预处理组大鼠逼尿肌细胞AI值均显著低于模型组(P0.01)。对照组和预处理组大鼠逼尿肌细胞bax表达显著高于模型组(P0.01),逼尿肌细胞bcl-2表达显著低于模型组(P0.01)。对照组和预处理组大鼠脊髓BDNF、Trk B表达显著低于模型组(P0.01),脊髓P2X3蛋白表达显著高于模型组(P0.01)。结论电针预处理可以改善布比卡因中毒大鼠排尿功能障碍,其作用机制可能与调控膀胱逼尿肌Bcl-2/Bax及脊髓BDNF、Trk B、P2X3蛋白表达相关。
[Abstract]:Objective to investigate the effect of electroacupuncture intervention on urinary dysfunction of bupivacaine and its related mechanism. Methods Sixty clean SD male rats aged 6 weeks were divided into control group, model group and pretreatment group with 20 rats in each group. The model group and pretreatment group were injected with spinal anesthesia to establish the model of bupivacaine poisoning in rats. Rats in the model group were pretreated with electroacupuncture before modeling. The parameters of bladder manometry were measured and recorded in each group. The apoptosis rate of detrusor muscle cells and the expression of bcl-2 in the cells of detrusor muscle were detected and compared by immunohistochemical method. The expression of BDNF Trk B and P2X3 protein in spinal cord of rats in each group was detected and compared by Western blot method. Results the urine volume, residual urine volume and bladder volume in the control group and pretreatment group were significantly higher than those in the model group, and the intravesical pressure was significantly lower than that in the model group. The AI of detrusor cells in control group and preconditioning group was significantly lower than that in model group (P 0.01). The bax expression of detrusor cells in the control group and preconditioning group was significantly higher than that in the model group, and the bcl-2 expression in the detrusor cells was significantly lower than that in the model group. The expression of BDNF Trk B in the spinal cord of the control group and preconditioning group was significantly lower than that of the model group, and the expression of P2X3 protein in the spinal cord was significantly higher than that in the model group. Conclusion electroacupuncture preconditioning can improve urination dysfunction in rats with bupivacaine poisoning, and its mechanism may be related to the regulation of Bcl-2/Bax in bladder detrusor muscle and BDNF Trk BP2X3 protein expression in spinal cord.
【作者单位】：河北省医科大学第三医院;
【分类号】：R245

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