针刺对支气管哮喘大鼠肺组织P-AKT蛋白表达的调控

发布时间：2018-05-06 07:48

本文选题：针刺 + 哮喘　；参考：《辽宁中医杂志》2017年10期

【摘要】：目的:探讨针刺对哮喘大鼠肺组织中P-AKT蛋白表达的影响。方法:将40只SPF级雄性SD大鼠随机分为空白组、哮喘模型组、哮喘模型针刺组和哮喘模型雾化阻断剂组,每组10只。各组分别给予相应干预后,用HE染色观察大鼠肺组织形态学改变,Western Blot法和免疫组织化学法检测大鼠肺组织P-AKT蛋白的表达。结果:HE染色显示哮喘模型组大鼠气道周围有多种炎症细胞的浸润和聚集,支气管平滑肌痉挛,官腔狭窄;针刺组和雾化阻断剂组有少量炎症细胞,官腔狭窄管壁增厚程度较模型组减轻。Western Blot和免疫组化法检测示哮喘模型组P-AKT蛋白表达较空白组、针刺组、雾化阻断剂组升高(P0.05);针刺后P-AKT蛋白表达降低(P0.05),雾化阻断剂组与空白组无差异(P0.05)。结论:针刺可降低大鼠肺组织中P-AKT蛋白表达以减轻炎症反应和气道重塑。
[Abstract]:Objective: to investigate the effect of acupuncture on the expression of P-AKT protein in lung tissue of asthmatic rats. Methods: forty SPF male Sprague-Dawley rats were randomly divided into blank group, asthma model group, asthma model acupuncture group and asthma model atomization blocker group with 10 rats in each group. The morphological changes of lung tissue were observed by HE staining and the expression of P-AKT protein in rat lung tissue was detected by immunohistochemical method and Western Blot method. Results in the asthmatic model group, there were many inflammatory cells infiltrating and aggregating around the airway, bronchial smooth muscle spasm and stenosis of the functional cavity, and a small number of inflammatory cells in the acupuncture group and atomization blocker group. Compared with the model group, the thickening degree of the stenosis wall of the functional cavity decreased. Western Blot and immunohistochemical method showed that the expression of P-AKT protein in the asthmatic model group was higher than that in the blank group and the acupuncture group. The expression of P-AKT protein decreased after acupuncture, but there was no difference between the atomization blocker group and the blank group. Conclusion: acupuncture can reduce the expression of P-AKT protein in rat lung tissue and alleviate inflammation and airway remodeling.
【作者单位】：华北理工大学中医学院;
【分类号】：R245

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