血水同治法治疗溃疡性结肠炎的临床与实验研究

发布时间：2018-05-06 08:46

本文选题：血水同病 + 血水同治法　；参考：《成都中医药大学》2016年硕士论文

【摘要】：目的：通过采用“血水同治”法的代表方剂当归芍药散治疗溃疡性结肠炎(Ulcerative Colitis, UC),观察其对结肠粘膜水通道蛋白3、4和血液流变学等相关指标的影响,初探“血水同治”法对UC“血”和“水”的调节机制以及UC的发病机制。方法：(1)在临床方面,将60例UC患者简单随机分为治疗组和对照组,各30例。治疗组每日口服当归芍药散水煎剂,并用其水煎液每晚保留灌肠1次。对照组口服柳氮磺吡啶肠溶片,再用氢化可的松每晚保留灌肠1次。各组治疗前后均检测血液流变学指标,治疗1周后观察两组患者血液流变学情况。(2)实验方面,将60只SD雄性大鼠随机分为正常组、模型组、当归芍药散高、中、低剂量组和对照组,共6组,每组10只。除正常组外其余50只均建立UC模型,造模成功后,每日予以当归芍药散高、中、低剂量组不同剂量中药煎剂灌胃,SASP组予以柳氮磺吡啶混悬液灌胃,模型组及正常组每日给予等容积的生理盐水灌胃,各组灌胃均1次/日,连续治疗两周后,腹主动脉采血备检,再处死动物留取病变结肠做标本备检。结果： (1)在临床方面,治疗组和对照组在治疗前血液流变学指标均有异常增高,经治疗后,各组血液流变学指标均有降低,治疗前后比较差异有统计学意义(P0.05)。(2)在实验方面,模型组AQP3、AQP4阳性细胞表达量、平均光密度值显著下降,低于正常组(P0.05),当归芍药散高、中剂量组和阳性对照组大鼠结肠组织AQP3、AQP4表达量和平均光密度值较模型组显著升高,其间差异有统计学意义(P0.05)。结论：UC患者存在血液高凝状态,“血瘀”在UC发病中占有重要地位；UC患者可能存在水液代谢障碍。“血水同病”可能是UC的发病机制。血水同治法治疗UC效果显著,其机制可能是通过改善UC血液流变学及上调结肠AQP3、AQP4表达来实现的。
[Abstract]:Objective: to observe the effect of Danggui Shaoyao Powder (Danggui Shaoyao Powder), the representative prescription of "Blood and Water treatment", on the colon mucosal aquaporin-3 and hemorheology in the treatment of Ulcerative Colitis of ulcerative colitis (UCX). The regulation mechanism of UC blood and water and the pathogenesis of UC were discussed. Methods 60 patients with UC were randomly divided into treatment group (n = 30) and control group (n = 30). In the treatment group, Danggui Shaoyao Powder decoction was taken daily, and its decoction was retained once a night. The control group was treated with sulfasalopyridine enteric-coated tablets and hydrocortisone enema once a night. After 1 week of treatment, 60 SD male rats were randomly divided into normal group, model group, Danggui Shaoyao powder, middle and middle groups. Low dose group and control group, 6 groups, 10 rats in each group. The UC model was established in the other 50 rats except the normal group. After the model was made successfully, Danggui Paeoniflorin Powder was given daily to SASP group with different dosages of traditional Chinese medicine decoction and SASP. The rats in the model group and the normal group were given the same volume of normal saline daily. The rats in each group were given intragastric perfusion once a day. After two weeks of continuous treatment, the abdominal aorta was collected for blood preparation, and then the animals were sacrificed to take samples from the diseased colon for preparation. Results: in the clinical aspect, the hemorheology indexes of the treatment group and the control group were increased before and after treatment. After treatment, the hemorheological indexes of each group were all decreased, and the difference before and after treatment was statistically significant (P 0.05). The expression of AQP3AAQP4 positive cells in the model group was significantly lower than that in the normal group (P 0.05). The expression of AQP3AAQP4 and the average optical density in the colonic tissue of the middle dose group and the positive control group were significantly higher than those in the model group. The difference was statistically significant (P 0.05). Conclusion Blood hypercoagulability exists in the patients with UC, and blood stasis plays an important role in the pathogenesis of UC. The pathogenesis of UC may be the same disease of blood and water. The effect of simultaneous treatment of blood and water on UC is remarkable, and the mechanism may be achieved by improving the hemorheology of UC and upregulating the expression of AQP3 and AQP4 in colon.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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