电针治疗失眠症的交感—肾上腺髓质系统机制研究

发布时间：2018-05-12 22:06

本文选题：失眠 + 电针　；参考：《成都中医药大学》2016年硕士论文

【摘要】：目的：观察蓝斑去甲肾上腺素在失眠中的作用,电针“神门”、“三阴交”对失眠模型大鼠睡眠、焦虑抑郁状态以及中枢、血浆儿茶酚胺类神经递质的影响,探索电针治疗失眠的交感-肾上腺髓质系统机制。方法：将健康SPF级SD大鼠以体重、高架十字迷宫实验、鼠尾悬挂实验数据为基准,筛选合格动物,并将其随机分为空白组、假注射组、NE模型组、NE模型电针组。NE模型组和NE模型电针组进行侧脑室微量注射去甲肾上腺素,假注射组插入微量注射针头但不注射药物,空白组常规饲养。侧脑室注射后,各组进行24小时自发活动检测、高架十字迷宫实验以及鼠尾悬挂实验。检测后,NE模型电针组行“神门”、“三阴交”电针治疗,连续治疗4天,其余各组同步抓取固定不治疗。电针治疗后,各组先进行24小时自发活动、高架十字迷宫实验、鼠尾悬挂实验检测,再进行丘脑、脑干去甲肾上腺素及血浆去甲肾上腺素、肾上腺素、多巴胺含量检测。结果：(1)各组基线情况：空白组、假注射组、NE模型组、NE模型电针组大鼠在体重、鼠尾悬挂实验静止时间以及高架十字迷宫实验OE%、OT%方面,组间比较无统计学差异(P0.05)。(2)侧脑室注射后行为学情况：侧脑室注射后,各组大鼠在鼠尾悬挂实验静止时间方面,组间差异无统计学意义(P0.05)；而在24小时自发活动、高架十字迷宫实验OE%和OT%方面,组间差异均具有统计学差异(P0.05)。差异具体表现为：①24小时自发活动：NE模型组、NE模型电针组白昼活动量与空白组、假注射组相较显著增加(P0.05)、夜晚活动量显著减少(P0.05),假注射组白昼活动量、夜晚活动量与空白组相较无明显差异(P0.05)；②高架十字迷宫实验：NE模型组、NE模型电针组OE%和0T%与空白组、假注射组相较显著降低(P0.05),假注射组OE%和OT%与空白组相较无明显改变(P0.05)。(3)电针治疗后行为学情况：电针治疗后,各组大鼠在鼠尾悬挂实验静止时间方面,组间差异无统计学意义(P0.05)；而在24小时自发活动、高架十字迷宫实验OE%和OT%方面,组间差异均具有统计学差异(P0.05)。差异具体表现为：①24小时自发活动：NE模型组白昼活动量与空白组、假注射组相较显著增加(P0.05)、夜晚活动量显著减少(P0.05),NE模型电针组白昼活动量与NE模型组相较明显减少(P0.05)、夜晚活动量明显增加(P0.05),NE模型电针组白昼活动量、夜晚活动量与空白组、假注射组相较无明显差异(P0.05),’假注射组白昼活动量、夜晚活动量与空白组相较无明显差异(P0.05)；②高架十字迷宫实验：NE模型组OE%和OT%与空白组、假注射组相较明显降低(P0.05),NE模型电针组OE%和OT%与NE模型组相较明显升高(P0.05),NE模型电针组OE%和OT%与空白组、假注射组相较无明显差异(P0.05),假注射组OE%和OT%与空白组相较无明显差异(P0.05)。(4)电针治疗后神经递质情况：电针治疗后,各组大鼠在丘脑、脑干去甲肾上腺素含量,血浆去甲肾上腺素、肾上腺素、多巴胺含量方面,组间差异均具有统计学差异(P0.05)。差异具体表现为：①丘脑、脑干去甲肾上腺素含量：NE模型组与空白组、假注射组相较明显增加(P0.05),NE模型电针组与NE模型组相较明显减少(P0.05),NE模型电针组与空白组、假注射组相较无明显差异(P0.05),假注射组与空白组相较无明显差异(P0.05);②血浆去甲肾上腺素、肾上腺素、多巴胺含量：NE模型组与空白组、假注射组相较明显升高(P0.05),NE模型电针组与NE模型组相较明显减低(P0.05),NE模型电针组与空白组、假注射组相较无明显差异(P0.05),假注射组与空白组相较无明显差异(P0.05)。结论：侧脑室微量注射去甲肾上腺素建立失眠模型具有可行性,模型大鼠具有交感-肾上腺髓质系统兴奋、睡眠障碍以及焦虑等类似人类失眠的表现,蓝斑去甲肾上腺素可兴奋交感-肾上腺髓质系统引发失眠；电针神门、三阴交通过调节蓝斑去甲肾上腺素含量、改善交感-肾上腺髓质系统功能状态治疗失眠。
[Abstract]:Objective: To observe the effect of norepinephrine on insomnia, the effect of electroacupuncture "Shen gate" and "Sanyinjiao" on sleep, anxiety and depression and the center, plasma catecholamine neurotransmitters in the rat model of insomnia model, and explore the mechanism of the sympathetic adrenomedullary system in the treatment of insomnia by electroacupuncture. Methods: the body of healthy SPF SD rats is in body. Heavy, elevated cross labyrinth test and rat tail suspension test data were used to screen qualified animals, and they were randomly divided into blank group, false injection group, NE model group, NE model electroacupuncture group.NE model group and NE model electroacupuncture group for lateral ventricle microinjection of norepinephrine, false injection group inserted micro injection needles but no drug injection, blank group. After the routine feeding, after the injection of the lateral ventricle, 24 hours of spontaneous activity detection, the viaduct test and the tail suspension test were carried out. After the test, the NE model electroacupuncture group was treated with the "Shen gate", "Three Yin cross" electroacupuncture treatment, and the other groups were treated for 4 days. The rest of the acupuncture groups were not treated at the same time. After the electroacupuncture treatment, 24 hours of spontaneous activity was carried out in each group. An elevated cross labyrinth test, a rat tail suspension test, and then a test of thalamus, norepinephrine and plasma norepinephrine, adrenaline and dopamine in the brain stem. Results: (1) the baseline conditions of each group: blank group, false injection group, NE model group, NE model electroacupuncture group in body weight, stern suspension experiment time and Viaduct The cross maze test OE%, OT%, there was no statistical difference between groups (P0.05). (2) after lateral ventricle injection, after injection of the lateral ventricle, there was no significant difference between the groups in the rest of the rat tail suspension experiment (P0.05), but in the 24 hour spontaneous activity, OE% and OT% in the elevated cross maze test, the difference between the groups There were statistical differences (P0.05). The differences were as follows: (1) 24 hours of spontaneous activity: the NE model group, the day activity of the NE model electroacupuncture group and the blank group, the false injection group increased significantly (P0.05), the night activity decreased significantly (P0.05), the false injection group was alive in the daytime, and there was no significant difference between the night activity and the blank group (P0.05). (2) the elevated cross maze test: NE model group, NE model electroacupuncture group OE% and 0T% and blank group, false injection group decreased significantly (P0.05), OE% and OT% in the false injection group were not significantly changed (P0.05). (3) the behavior after electroacupuncture treatment: after electroacupuncture treatment, the rest time of rats in rats tail suspension experiment, the difference between groups There was no statistical significance (P0.05), but in 24 hours of spontaneous activity, the differences in OE% and OT% were statistically different (P0.05). The differences were as follows: (1) 24 hours of spontaneous activity: the daytime activity in the NE model group and the blank group, the false injection group increased significantly (P0.05), and the activity at night decreased significantly (P0.05 In the NE model, the daytime activity of the electroacupuncture group decreased significantly (P0.05), the night activity increased significantly (P0.05), the daytime activity of the NE model electroacupuncture group, the night activity and the blank group, and the false injection group had no significant difference (P0.05), "the white day activity in the false injection group, and the night activity volume and the blank group had no significant difference (P0.05). (2) the elevated cross labyrinth test: the NE model group OE% and OT% and the blank group, the false injection group decreased significantly (P0.05), the OE% and OT% in the NE model group were significantly higher than the NE model group (P0.05), the NE model electroacupuncture group was OE% and OT% and blank groups, and the false injection group had no obvious difference. The difference (P0.05). (4) the neurotransmitter after electroacupuncture treatment: after electroacupuncture treatment, the differences in the content of thalamus, norepinephrine, norepinephrine, epinephrine and dopamine in the rats were all statistically different (P0.05). The difference specific manifestations were: (1) thalamus, brain stem norepinephrine content: NE model The group and the blank group, the false injection group increased significantly (P0.05), the NE model electroacupuncture group and the NE model group decreased significantly (P0.05). The NE model electroacupuncture group and the blank group, the false injection group were not significantly different (P0.05), the false injection group was not significantly different from the blank group (P0.05); (2) the plasma norepinephrine, adrenaline, dopamine content: NE The model group and the blank group, the false injection group increased significantly (P0.05), the NE model electroacupuncture group was significantly lower than the NE model group (P0.05). The NE model electroacupuncture group and the blank group, the false injection group were not significantly different (P0.05), the false injection group was not significantly different from the blank group (P0.05). Conclusion: the side ventricle microinjection of norepinephrine to establish the loss of norepinephrine. Sleep model is feasible, model rats have sympathetic adrenal medullary system excitement, sleep disorder and anxiety, such as the performance of human insomnia, blue norepinephrine can stimulate the sympathetic adrenal medulla system to cause insomnia; Electroacupuncture of the God gate, three yin traffic regulation of the locus methadinephrine content, the improvement of sympathetic adrenomedullary medulla The functional state of the system is used to treat insomnia.

【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R246.6

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