针刺俞、原、募穴对高尿酸血症大鼠SUA、XOD、ALP的影响

发布时间：2018-05-13 01:37

本文选题：针刺 + 高尿酸血症　；参考：《北京中医药大学》2017年硕士论文

【摘要】：研究目的:通过分别针刺大鼠肝、脾和肾的俞穴、原穴以及大、小肠的募穴这3组穴位,观察不同的穴位对于痛风早期高尿酸血症模型大鼠体内的SUA、XOD、ALP含量和肾脏指数的影响,以及对于肾脏的大体形态和肾脏细胞组织结构形态的影响,明确这3组针刺穴位对于痛风高尿酸血症的形成是否具有干预预防的作用,筛选针刺防治痛风高尿酸血症的最佳穴位,为临床治疗提供选穴依据。研究方法:将50只健康的、成年的雄性Wistar大鼠采取随机分组的方法,平均分成5组:空白组、模型组、俞穴组、原穴组、募穴组,每组10只。造模采用灌胃给药的方法制备高尿酸血症大鼠模型,造模剂为腺嘌呤(100mg/kg)加乙胺丁醇(250mg/kg)。5组同时于实验第1天开始造模,前21天每日灌胃一次造模剂,21天之后改为隔日灌胃一次,直到第90天实验结束。其中的俞穴组、原穴组和募穴组3组针刺干预组于造模的同时予以针刺治疗,隔日针刺1次,每次针刺15min,针刺10次作为1个疗程,每疗程结束后停止针刺10天,之后再进行下一疗程的干预,如此循环往复,到实验第90天结束,共3个疗程。实验结束后,5组大鼠统一取材,对于指标的检测,实验采用全自动生化分析仪检测SUA及ALP,免疫荧光法检测XOD,将大鼠肾脏做成石蜡切片,用HE染色法染色,再置于普通光学显微镜下观察其细胞组织结构。实验结果:与空白组相比较,模型组的SUA和XOD含量明显升高,肾脏指数也明显增大,肉眼观察肾脏体积增大,镜下观察肾脏切片肾小管扩张,炎性细胞浸润;与模型组相比较,俞穴组、原穴组和募穴组3组针刺干预组的SUA、XOD含量均有不同程度的降低,肾脏指数和肾脏体积也均有减小,镜下观察肾脏HE染色切片肾小管扩张程度降低,炎性细胞数量减少,胞浆空泡减少;3组针刺干预组之间相互比较,肾脏指数方面组间并无明显的差异性,募穴组、原穴组降低SUA、XOD含量的效果较俞穴组显著,其中募穴组在降低SUA含量方面的干预效果最明显;5组实验组之间ALP含量均互无明显的差异性。实验结论:(1)3组针刺干预组均能在不同程度上降低SUA和XOD的含量,并且均能有效地降低对肾脏的损害,减缓造模剂对肾脏细胞的正常形态和功能的侵蚀过程,在一定程度上成功地预防了高尿酸血症的形成,防止了肾损害,预防了痛风;(2)在降低SUA含量方面,干预效果的显著程度以募穴组为最佳,原穴组的效果次之,俞穴组的效果在3组中相对最不明显;在抑制X0D活性方面,募穴组和原穴组的干预效果要好于俞穴组;(3)5组实验组的ALP含量互无差异性,说明体内ALP含量的影响因素可能不仅是高尿酸血症,同时也是体内其他复杂变化综合作用的结果,具体原因还需做进一步的研究。
[Abstract]:Objective: to observe the effects of different acupoints on the content of SUAXODN ALP and renal index in rats with early gout hyperuricemia by acupuncture the points of liver, spleen and kidney at Shu point, original point, large point and small intestine acupoint, respectively, in order to observe the effect of different acupoints on the content of SUAXODN ALP in rats with early gout hyperuricemia. The effects of acupuncture on the gross morphology of kidney and the tissue structure of renal cells were also discussed. Whether the acupuncture points of these three groups could interfere with and prevent the formation of hyperuricemia in gout was determined. To select the best acupuncture points for prevention and treatment of hyperuricemia of gout. Methods: 50 healthy adult male Wistar rats were randomly divided into 5 groups: blank group, model group, Shu group, original acupoint group and mu-point group, with 10 rats in each group. The rat model of hyperuricemia was established by intragastric administration of adenine (100 mg / kg) and ethylamine butanol (250 mg / kg). The model was established on the first day of the experiment. After 21 days of oral administration of the model agent once a day, the rat model was given once every other day. Until the end of the 90 th day. Among them, the group of Shu, the group of original acupoint and the group of acupoint raising were treated with acupuncture at the same time when the model was made. The acupuncture was treated once every other day for 15 mins each time, 10 times as a course of treatment, and 10 days after the end of each course of treatment, the acupuncture was stopped for 10 days. Then the next course of intervention, so cycle, to the end of the experiment 90 days, a total of 3 courses. SUA and ALP were detected by automatic biochemical analyzer, XODs were detected by immunofluorescence, and the kidneys were made into paraffin sections and stained by HE staining. The tissue structure of the cells was observed under the ordinary optical microscope. Results: compared with the blank group, the contents of SUA and XOD in the model group were significantly increased, the renal index was also significantly increased, the size of the kidney was increased with the naked eye, the renal tubular dilatation and inflammatory cell infiltration were observed under microscope, and compared with the model group, The content of SUAXOD, renal index and renal volume in the three groups of acupuncture intervention group were all decreased, and the renal tubule dilatation degree was decreased and the number of inflammatory cells was decreased in HE stained sections of kidney under microscope. There was no significant difference in renal index between the three acupuncture intervention groups. The effect of reducing the XOD content in the acupoint group and the original acupoint group was significantly higher than that in the Shu group, but there was no significant difference in the renal index between the acupuncture intervention groups and the original acupoint group, but there was no significant difference in the renal index between the three groups. There was no significant difference in ALP content among the five experimental groups. Conclusion Acupuncture intervention group can decrease the content of SUA and XOD in different degree, and can effectively reduce the damage to kidney and the erosion process of normal morphology and function of kidney cells. To a certain extent, the formation of hyperuricemia was prevented, kidney damage was prevented, and gout was prevented. In the aspect of reducing SUA content, the intervention effect was the best in the control group, followed by the original point group. In inhibiting X0D activity, the intervention effect of the acupoint raising group and the original point group was better than that of the control group, and there was no difference in the ALP content between the two groups. It is suggested that the influencing factors of ALP content in vivo may be not only hyperuricemia, but also the comprehensive effect of other complex changes in vivo. The specific reasons need to be further studied.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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