银质针治疗对肌筋膜疼痛综合征大鼠脊髓神经递质表达的影响

发布时间：2018-05-13 11:11

本文选题：肌筋膜疼痛综合征 + 银质针　；参考：《贵州医科大学》2016年硕士论文

【摘要】：目的:观察银质针导热疗法对肌筋膜疼痛综合征(myofascial pain syndrome,MPS)大鼠脊髓水平神经递质的影响,初步探讨银质针治疗肌筋膜疼痛综合征是否有中枢镇痛机制的参与。方法:将24只健康雌性SD大鼠随机分为3组,每组8只:(1)正常组:正常饲养,不给予任何刺激;(2)模型组:采用打击右股内侧肌结合运动疲劳建立慢性肌筋膜疼痛综合征模型,而后正常饲养;(3)银质针组:肌筋膜疼痛综合征大鼠模型建立成功后,行银质针治疗。各组大鼠在治疗2周后测定热缩爪潜伏期、右股内侧肌肌电图自发电活动发生频率。取右股内侧肌,HE染色切片观察形态学变化,检测脊髓L4-6节段神经元型一氧化氮合酶(nNOS)、P物质(substance P,SP),及5-羟色胺(5-HT)的表达。结果:与正常组相比,模型组大鼠热缩爪潜伏期降低(p0.01);右股内侧肌自发电活动频率增加(p0.01);脊髓nNOS、SP表达升高(p0.01),差异有统计学意义。与模型组相比,银质针组热缩爪潜伏期明显升高(p0.01);右股内侧肌自发电活动频率明显降低(p0.01);脊髓nNOS及SP表达明显降低(p0.01);5-HT表达明显升高,差异有统计学意义。结论:1.银质针治疗可以增加脊髓后角5-HT及降低SP、nNOS的表达,提示可能通过对疼痛相关中枢神经递质的调节发挥治疗作用。2.银质针治疗可以改善肌筋膜疼痛综合征大鼠局部肌肉的病理状态,促进肌肉组织修复。3.银质针治疗能够减轻热痛觉敏感,提高热缩爪潜伏期。4.银质针治疗可减少肌筋膜疼痛综合征大鼠局部肌肉自发电活动。
[Abstract]:Objective: to observe the effect of silver acupuncture heat conduction therapy on spinal neurotransmitters in rats with myofascial pain syndromes (MPSs) and to explore whether there is a central analgesic mechanism in the treatment of myofascial pain syndrome. Methods: Twenty-four healthy female Sprague-Dawley rats were randomly divided into 3 groups, each group (n = 8) normal feeding without any stimulation (n = 2) model group: chronic myofascial pain syndrome model was established by attacking right medial femoris muscle combined with exercise fatigue. The model of myofascial pain syndrome was established successfully and then treated with silver acupuncture. After 2 weeks of treatment, the latent period of heat contraction claw and the frequency of autogeneration of right medial thigh muscle electromyography were measured. The morphological changes were observed by HE staining sections of the medial femoral muscle, and the expression of substance (PnNOSN) and serotonin (5-HT) in the L4-6 segment of spinal cord were detected. Results: compared with the normal group, the latent period of heat contraction paw was decreased in the model group, the frequency of autogeneration in the right medial femoral muscle was increased, and the expression of nNOS SP was increased in the spinal cord (P 0.01). Compared with the model group, the latent period of heat contraction claw in the silver needle group was significantly higher than that in the model group, the frequency of autogeneration in the right medial femoral muscle was significantly decreased, and the expression of nNOS and SP in the spinal cord was significantly decreased, and the expression of 5-HT in the spinal cord was significantly higher than that in the control group. Conclusion 1. Silver acupuncture can increase the expression of 5-HT and decrease the expression of SPN nNOS in the posterior horn of spinal cord, suggesting that it may play a therapeutic role by regulating the central neurotransmitters related to pain. Silver needle therapy can improve the pathological state of muscle in rats with myofascial pain syndrome and promote muscle tissue repair. Silver needle therapy can reduce the sensitivity of thermal pain and increase the latent period of heat contraction claw. 4. The treatment of silver acupuncture can reduce the autogeneration of muscle in rats with myofascial pain syndrome.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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