鼻敏感颗粒对变应性鼻炎大鼠Nods样模式识别受体的影响

发布时间：2018-05-16 03:26

本文选题：变应性鼻炎 + Nods样模式识别受体　；参考：《南京中医药大学》2016年博士论文

【摘要】：目的探讨鼻敏感颗粒对变应性鼻炎大鼠鼻粘膜中Nods样模式识别受体的表达的影响,并研究鼻敏感颗粒对变应性鼻炎大鼠外周血中IL-1β、IL-17、IL-18、TGF-β1细胞因子的表达的影响,以探索Nods样模式识别受体与变应性鼻炎发病机制的关系,并进一步研究鼻敏感颗粒治疗变应性鼻炎的作用机理。方法本研究包括两个方面：一是进行鼻敏感颗粒的药效学实验研究,包括抗炎、镇痛、抗过敏实验；二是以48只SD大鼠为实验对象,分为空白组、模型组、氯雷他定组与鼻敏感颗粒组,每组12只,建立变应性鼻炎大鼠模型后,两组用药组分别使用氯雷他定和鼻敏感颗粒对其进行药物治疗,观察鼻敏感颗粒对变应性鼻炎大鼠症状体征变化及Nods样模式识别受体的影响。结果1.鼻敏感颗粒对大鼠具有抗炎、镇痛、抗过敏作用；2.症状体征评分：鼻敏感颗粒对变应性鼻炎大鼠喷嚏、擦鼻以及流涕症状有良好的治疗作用,与模型组相比,差异有统计学意义(P0.01)。3.病理学检查：鼻敏感颗粒对变应性鼻炎大鼠鼻腔粘膜炎症表现有明显改善作用,与模型组相比,差异有统计学意义(P0.01)。4.细胞因子检测：鼻敏感颗粒对变应性鼻炎大鼠外周血中IL-1β、IL-17、IL-18有明显抑制作用,对TGF-β1有明显促进作用。5.免疫组化检测：变应性鼻炎大鼠鼻粘膜中三种Nods样模式识别受体呈现高表达状态,而鼻敏感颗粒可明显抑制三种Nods样模式识别受体的表达,与模型组相比,差异有统计学意义(P0.01)。6.Western-Blot检测：变应性鼻炎大鼠鼻粘膜中Nod1、Nod2、 Nalp3蛋白表达明显高于空白组大鼠,经过鼻敏感颗粒治疗后,三种Nods样模式识别受体蛋白含量均明显降低,与模型组相比,差异有统计学意义(P0.01)。7.RT-PCR检测：变应性鼻炎大鼠鼻粘膜中存在Nod1mRNA、Nod2mRNA、Nalp3mRNA表达,经鼻敏感颗粒灌胃治疗后三种Nods样模式识别受体的iRNA表达量水平明显降低,与模型组相比,差异有统计学意义(P0.01)。结论1..Nod1、Nod2、Nalp3在变应性鼻炎大鼠模型中均有表达,变应性鼻炎大鼠模型组中的蛋白及nRNA表达明显高于空白组,提示Nod1、Nod2、Nalp3可能参与变应性鼻炎的发病。2.鼻敏感颗粒具有抗炎、镇痛、抗过敏作用,变应性鼻炎大鼠经过鼻敏感颗粒的治疗后,其过敏症状明显减轻,鼻粘膜中Nod1、Nod2、Nalp3的表达明显低于模型组,外周血中的IL-1β、IL-17、IL-18含量明显减低,TGF-β1含量明显升高。提示鼻敏感颗粒可能通过影响ods样模式识别受体对Th17/Treg免疫平衡的调控作用,参与变应性鼻炎的治疗。为鼻敏感颗粒的临床科学应用提供新的依据。
[Abstract]:Objective to investigate the effect of rhinosensitive granules on the expression of Nods like pattern recognition receptor in nasal mucosa of rats with allergic rhinitis, and to study the effect of rhinosensitive granules on the expression of IL-1 尾 -IL-17 IL-18 TGF- 尾 1 cytokines in peripheral blood of allergic rhinitis rats. To explore the relationship between Nods like pattern recognition receptor and the pathogenesis of allergic rhinitis, and to further study the mechanism of Bi-sensitive granule in the treatment of allergic rhinitis. Methods the study included two aspects: one was to study the pharmacodynamics of nasal sensitive granules, including anti-inflammatory, analgesic and anti-allergic experiments, the other was to divide 48 SD rats into blank group and model group. After the establishment of allergic rhinitis rat model, the two groups were treated with loratadine and rhinosensitive granules, respectively, in each group, 12 rats in each group were treated with loratadine and nasal sensitive granules, respectively, after the establishment of allergic rhinitis model, the two groups were treated with loratadine and nasal sensitive granules respectively. To observe the effect of Bi-sensitive granule on the changes of symptoms and signs and Nods like pattern recognition receptor in allergic rhinitis rats. Result 1. Rhinosensitive granules have anti-inflammatory, analgesic and anti-allergic effects in rats. Score of symptoms and signs: rhinosensitive granules had a good therapeutic effect on sneezing, nasal rub and runny symptoms in allergic rhinitis rats, compared with model group, the difference was statistically significant (P 0.01). Pathological examination: rhinosensitive granules can obviously improve the nasal mucosal inflammation in allergic rhinitis rats, compared with the model group, the difference is statistically significant (P 0.01). 4. Detection of cytokines: rhinosensitive granules had obvious inhibitory effect on IL-1 尾 -IL-17 IL-18 in peripheral blood of allergic rhinitis rats, and significantly promoted TGF- 尾 1. Immunohistochemistry: the expression of three Nods like pattern recognition receptors in the nasal mucosa of allergic rhinitis rats showed high expression, while nasal sensitive granules could significantly inhibit the expression of three Nods like pattern recognition receptors, compared with the model group. The difference was statistically significant (P 0.01) .6.Western-Blot: the expression of Nod1nd2 and Nalp3 protein in nasal mucosa of allergic rhinitis rats was significantly higher than that of blank rats. After treatment with nasal sensitive granules, the protein content of three Nods like pattern recognition receptors was significantly decreased, compared with model group, and compared with the model group, the expression of Nod1 and Nod2 protein in the nasal mucosa of allergic rhinitis rats was significantly lower than that in the control group. The expression of Nod1mRNA-Nod2mRNA-Nalp3 mRNA was found in nasal mucosa of allergic rhinitis rats. The iRNA expression level of three Nods like pattern recognition receptors was significantly decreased after the treatment of nasal sensitive granules, compared with the model group, the expression of Nalp3 mRNA in the nasal mucosa of rats with allergic rhinitis was significantly lower than that in the model group. The difference was statistically significant (P 0.01). Conclusion the expression of Nalp3 in allergic rhinitis rat model is higher than that in control group, suggesting that Nod1 / Nod2nalp3 may be involved in the pathogenesis of allergic rhinitis. [conclusion] the expression of protein and nRNA in allergic rhinitis model group is significantly higher than that in control group, suggesting that Nod1 / Nod2Nalp3 may be involved in the pathogenesis of allergic rhinitis. Rhinosensitive granules had anti-inflammatory, analgesic and anti-allergic effects. The allergic symptoms of allergic rhinitis rats were alleviated after treatment, and the expression of Nod1, Nod2Nalp3 in nasal mucosa was significantly lower than that in the model group. The level of IL-1 尾 -17 IL-18 in peripheral blood decreased significantly, and the level of TGF- 尾 1 increased significantly. The results suggest that rhinosensitive granules may be involved in the treatment of allergic rhinitis by affecting the regulation of Th17/Treg immune balance by ods like pattern recognition receptor. To provide a new basis for clinical scientific application of nasal sensitive granules.
【学位授予单位】：南京中医药大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R276.1

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