维药对叶大戟质量标准研究

发布时间：2018-05-17 16:49

  本文选题：对叶大戟 + 质量评价　； 参考：《新疆师范大学》2016年硕士论文

【摘要】：目的:对叶大戟植物记载于《中国植物志》第44(3)卷,其中只收录了对叶大戟的名称、性状、产地、来源、鉴别。卫生部颁标准《维药分册》(31)收录了对叶大戟果性状、鉴别、杂质和水分检查、功能与主治。对叶大戟现阶段未有相应的质量标准要求,通过对叶大戟药材化学成分及质量标准进行科学研究,能更好的开发这一种新疆特色维吾尔医药材。方法:化学成分系统预试法初步判断对叶大戟药材中的化学成分种类;10批对叶大戟药材中水分、浸出物、总灰分、酸不溶性物质进行测定。根据2015版《中国药典》要求,采用ICP-AES法测定Fe、Cu、Zn、Cr;石墨炉原子吸收法测定Pb和Cd.;原子荧光法测定As和Hg进行含量测定。利用紫外可见分光光度仪测定总多酚、总黄酮的含量。采用红外光谱和化学计量学分析方法对10批对叶大戟药材及其提取物的红外光谱规律进行探究。HPTLC法对10批对叶大戟药材中3种有效成分山奈酚、莨菪亭、香草酸进行定性研究;采用HPLC测定对叶大戟中三种有效成分含量。结果:化学成分系统预试结果表明对叶大戟化学成分主要含有黄酮类、酚类、糖类、生物碱等.10批对叶大戟药材水分、总灰分、水溶性浸出物和醇溶性浸出物平均值分别为:5.74%、11.53%、17.68%、14.82%;采用ICP-AES、石墨炉原子吸收、原子荧光测定10批对叶大戟中8种规定元素(Fe、Cu、Zn、Cr、Pb、Cd、As、Cr)的含量,药材中8种元素含量均符合2015版《中国药典》元素含量要求;通过对10批对叶大戟提取液中总多酚、总黄酮进行提取工艺优化,得到总多酚和总黄酮最佳提取工艺条件分别是:乙醇浓度70%、料液比1:40、温度45℃;超声时间30 min和乙醇浓度70%、料液比1:35、温度35℃、超声时间40 min;含量分别在9.89-14.33和18.01-23.14 mg/g之间。10批对叶大戟中红外吸收峰的峰数、峰位、峰形、峰强度存在一定的差异,通过对样品红外光谱的比较,峰:3391cm-1、2927cm-1、1324cm-1、1240cm-1可作为对叶大戟药材共有特征峰,采用主成分分析和聚类分析法将10批对叶大戟进行分类;10批对叶大戟中山奈酚、莨菪亭和香草酸成分薄层分离效果较好,可作为对叶大戟质量标准评价的一个指标。建立了HPLC法测定对叶大戟中山奈酚、莨菪亭、香草酸含量,为对叶大戟药用提供了理论依据。结论:本研究初步探索了维药对叶大戟的质量评价方法,建立了对叶大戟中香草酸、莨菪亭、山奈酚的含量测定方法,测定了对叶大戟中不同的元素含量,为维药对叶大戟质量评价研究提供科学依据。
[Abstract]:Objective: halberd was recorded in the Chinese Flora 443, which contains only the name, character, origin, origin and identification of Euphorbia. The standard issued by the Ministry of Health, "Veterinary Medicine Partition", includes the characters, identification, impurity and moisture examination, function and main treatment of Euphorbia esculentum. There is no corresponding quality standard for Euphorbia esculentata at the present stage. Through the scientific research on the chemical composition and quality standard of Euphorbia esculentata, this kind of Uygur medical material with Xinjiang characteristics can be better developed. Methods: 10 batches of chemical constituents of Euphorbia esculentata were determined by the method of chemical composition system pretest. The contents of water, extract, total ash and acid insoluble substances in Euphorbia esculentata were determined in 10 batches. According to the requirements of Chinese Pharmacopoeia 2015 edition, ICP-AES method was used to determine the content of FebCuCuznzncr, graphite furnace atomic absorption spectrometry (GFAAS) to determine Pb and Cd.and atomic fluorescence spectrometry (AFS) to determine the contents of as and Hg. The contents of total polyphenols and total flavonoids were determined by UV-vis spectrophotometer. Infrared spectroscopy and chemometrics were used to study the infrared spectra of 10 batches of Euphorbia esculentata and its extracts. HPTLC was used to qualitatively study the three active components of Euphorbia mongolicum, scopoletin and vanillic acid in 10 batches of Euphorbia officinalis. The content of three active components in Euphorbia davidiana was determined by HPLC. Results: the chemical composition system showed that the chemical constituents of Euphorbia esculentum mainly contained flavonoids, phenols, carbohydrates, alkaloids and so on. The average values of water-soluble extracts and alcohol-soluble extracts were 11.53 and 17.68, respectively. ICP-AES, graphite furnace atomic absorption spectrometry and atomic fluorescence spectrometry were used to determine the contents of 8 specified elements in 10 batches of Euphorbia trifoliata. The total polyphenols and flavonoids in 10 batches of Euphorbia lobata extract were optimized by the content of 8 elements in the 2015 edition of Chinese Pharmacopoeia, and the contents of 8 elements in the medicinal materials met the requirements of the 2015 edition of the Chinese Pharmacopoeia. The optimum extraction conditions of total polyphenols and total flavonoids were as follows: ethanol concentration 70, ratio of material to liquid 1: 40, temperature 45 鈩，

本文编号：1902116