Bcl-2、Bak与骨质疏松症及其中医证型的相关性研究

发布时间：2018-05-18 09:36

本文选题：Bcl-2 + Bak　；参考：《广州中医药大学》2016年硕士论文

【摘要】：目的：本研究试图从基因水平探讨骨质疏松症发病原因,丰富骨质疏松症的病因理论；并从宏观和微观角度探讨骨质疏松症的中医辩证分型的物质基础,为中医药防治骨质疏松症提供可靠的科学依据。方法：自2014年08月至2015年12月份期间,将符合纳入标准的患者进行髋部手术时收集松质骨标本；分别采用定量PCR检测和Western Blot检测两组病例骨标本的抗细胞凋亡的Bcl-2基因和促细胞凋亡的Bak的基因及其调控的线粒体凋亡途径关键因子Cyt C、Apaf-1和Caspase-9的表达。数据处理、统计,分析指标与腰椎骨密度及中医证型的相关性。结果：本研究骨质疏松症患者61例(肝肾阴虚29例,脾肾阳虚32例),非骨质疏松症患者33例。男33例,女61例。年龄为47-93岁,平均年龄70.81±11.28岁。骨质疏松组与非骨质疏松组比较,腰1-4椎体骨密度及其平均值、腰椎T-score差异均具有统计学意义(P0.01)。非骨质疏松组患者年龄比骨质疏松组低,差异具有统计学意义(p＜0.05)；骨质疏松组的身高比非骨质疏松组低,差异具有统计学意义(P0.05)；骨质疏松组患者体重、体重指数(BMI)比非骨质疏松组低,差异具有统计学意义(P〈0.01)。不同中医证型两组之间年龄、身高、体重、体重指数差异均无统计学意义(P0.05)。非骨质疏松组Bcl-2表达高于骨质疏松组,骨质疏松组Bak及CytC、Apaf-1和Caspase-9的表达高于非骨质疏松组,Bcl-2基因与腰椎平均骨密度及腰椎T值呈正相关,Bak、CytC、Apaf-1和Caspase-9则与之呈负相关,相关性均具有统计学意义(P0.01)。骨质疏松症不同中医证型之间腰1-4椎体骨密度及其平均值、T-score差异均无有统计学意义。骨质疏松症不同中医证型的Bcl-2、Bak、 Cyt C、Apaf-1和Caspase-9差异均无统计学意义(P〉0.05)。结论：①Bcl-2表达与腰椎骨密度呈正相关,Bak、Cyt C、Apaf-1和Caspase-9的表达则与之呈负相关。②Bcl-2与Bak、CytC、Apaf-1和Caspase-9的表达与骨质疏松症中医证型相关性无统计学意义。
[Abstract]:Objective: this study attempts to explore the pathogenesis of osteoporosis at the gene level, enrich the etiological theory of osteoporosis, and explore the material basis of TCM dialectical classification of osteoporosis from macro and micro perspectives. To provide a reliable scientific basis for the prevention and treatment of osteoporosis by traditional Chinese medicine. Methods: from August 2014 to December 2015, cancellous bone specimens were collected from patients who met the inclusion criteria during hip surgery. Quantitative PCR and Western Blot were used to detect the expression of anti-apoptotic Bcl-2 gene and apoptosis-promoting Bak gene and Cyt CnApaf-1 and Caspase-9, respectively. Data processing, statistics, and analysis of the correlation between lumbar bone mineral density and TCM syndromes. Results: there were 61 cases of osteoporosis (29 cases of liver and kidney yin deficiency, 32 cases of deficiency of spleen and kidney yang, 33 cases of non-osteoporosis). There were 33 males and 61 females. The average age was 70.81 卤11.28 years. The bone mineral density (BMD) and average value of lumbar vertebrae and lumbar T-score in osteoporosis group were significantly higher than those in non-osteoporosis group (P 0.01). The age of non-osteoporosis group was lower than that of osteoporosis group, the difference was statistically significant (P < 0.05), the height of osteoporosis group was lower than that of non-osteoporosis group, and the difference was statistically significant (P 0.05). Body mass index (BMI) was significantly lower than that in non-osteoporosis group (P < 0.01). There was no significant difference in age, height and body mass index between the two groups. The expression of Bcl-2 in non-osteoporosis group was higher than that in osteoporosis group, and the expression of Bak, CytCnApaf-1 and Caspase-9 in osteoporosis group was higher than that in non-osteoporosis group. The expression of Bcl-2 gene was positively correlated with the mean bone mineral density of lumbar vertebrae and T value of lumbar vertebrae, but negatively correlated with the expression of Bak, CytCnApaf-1 and Caspase-9 in osteoporosis group. The correlation was statistically significant (P 0.01). There was no significant difference in bone mineral density (BMD) and T-score of lumbar 1-4 vertebrae between different TCM syndromes of osteoporosis. There was no significant difference in Bcl-2P, Cyt Apaf-1 and Caspase-9 between different TCM syndromes of osteoporosis (P > 0.05). Conclusion the expression of Bcl-2 was positively correlated with bone mineral density of lumbar vertebrae, but the expression of Apaf-1 and Caspase-9 were negatively correlated with BMD. 2 the expressions of Bcl-2 and Caspase-9 were not significantly correlated with the TCM syndrome type of osteoporosis.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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