穴位局部沉默对训练大鼠IL-2功能影响的研究

发布时间：2018-05-18 15:45

本文选题：Cx + 免疫功能　；参考：《中华中医药学刊》2017年01期

【摘要】：目的:研究阻断足三里穴对训练大鼠IL-2功能的影响。方法:将60只大鼠随机分为质粒组、阴性对照组、生理盐水组和空白组4组,并建立运动模型,对于质粒组大鼠足三里穴加压注射干扰质粒,20μg,阴性对照组大鼠足三里穴注射阴性对照质粒,每穴20μg;生理盐水组大鼠足三里穴注射生理盐水20μL;1天2次,连续注射14 d,并检测其淋巴细胞增值反应、IL-2的浓度等免疫学指标。结果:质粒组大鼠的淋巴细胞反应明显降低,质粒组(0.409±0.031)与阴性对照组(0.578±0.054)、生理盐水组(0.538±0.023)、空白组(0.552±0.042)相比较,差异均有显著性(P0.05)。质粒组大鼠的IL-2浓度明显减少,质粒组(424.12±85.36)与阴性对照组(594.12±74.17)、生理盐水组(589.30±77.15)、空白组(578.30±87.25)相比较,差异均有显著性(P0.05)。结论:经穴阻断可以降低训练大鼠的免疫功能,Cx43基因是经络活动的关键因素之一。
[Abstract]:Objective: to study the effect of blocking Zusanli (Zusanli) on IL-2 function in training rats. Methods: sixty rats were randomly divided into four groups: plasmid group, negative control group, normal saline group and blank group. For the rats in the plasmid group, 20 渭 g of interference plasmid was injected under pressure at Zusanli point, 20 渭 g of each point in the negative control group was injected into Zusanli point of the negative control group, and 20 渭 L of normal saline was injected twice a day at Zusanli point of rats in the saline group. After 14 days of continuous injection, the immunological indexes such as the concentration of IL-2 in lymphocyte proliferation reaction were measured. Results: the lymphocyte reaction of the plasmid group was significantly lower than that of the negative control group (0.409 卤0.031), the negative control group (0.578 卤0.054), the saline group (0.538 卤0.023) and the blank group (0.552 卤0.042). The concentration of IL-2 in plasmid group (424.12 卤85.36) was significantly lower than that in negative control group (594.12 卤74.17), normal saline group (589.30 卤77.15) and blank group (578.30 卤87.25). Conclusion: the blocking of meridian points can reduce the immune function of training rats, Cx43 gene is one of the key factors of meridian activity.
【作者单位】：滨州医学院解剖教研室;滨州医学院基础学院;
【基金】：国家自然科学基金项目(81001562) 山东省自然科学基金项目(ZR2011HQ041)
【分类号】：R245

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