益气养阴化瘀通络法对2型糖尿病大鼠胸主动脉病变的干预研究

发布时间：2018-05-24 12:52

本文选题：益气养阴 + 化瘀通络　；参考：《南京中医药大学》2016年硕士论文

【摘要】：本论文主要分为理论研究与实验研究两大部分。理论部分系统整理了中医学和西医学有关2型糖尿病(T2DM)大血管病变的文献,并对益气养阴化瘀通络法防治T2DM大血管病变进行了系统深入探讨。实验部分建立T2DM大鼠模型,观察通心络对T2DM大鼠胸主动脉的影响。目的：观察通心络对T2DM大鼠空腹血糖(FPG)、血脂、超氧化物歧化酶(SOD)、丙二醛(MDA)、活性氧簇(ROS)、糖基化终末产物(AGEs)和大鼠胸主动脉中AGEs蛋白表达的影响,探讨益气养阴化瘀通络法防治T2DM大血管病变的作用机制。方法：将80只雄性Wistar大鼠适应性饲养1周,然后随机分成实验组(70只)和空白组(10只)。实验组喂以高脂高糖饲料,空白组用常规标准饲料喂养4周,共5周,5周末对实验组的大鼠进行一次性腹腔注射新鲜配置的1%STZ (30mg/kg)柠檬酸-柠檬酸钠缓冲液,空白组注射同等剂量的柠檬酸-柠檬酸钠缓冲液。3天后对大鼠断尾采血,测定空腹血糖,连续两次空腹血糖耋16.7mmol/L,视为建模成功。除去造模失败和死去的大鼠,将成模的56只大鼠随机分为模型组(n=14),辛伐他汀对照组(n=14),通心络高剂量治疗组(n=14),通心络低剂量治疗组(n=14)。继续喂以高糖高脂饲料至第9周。然后对通心络高、低剂量组和辛伐他汀组大鼠进行连续给药至第21周。21周末检测大鼠的FPG、血脂、SOD、MDA、ROS、AGEs和胸主动脉中AGEs蛋白表达水平。结果：体重,通心络高剂量治疗组高于模型组和辛伐他汀对照组(P0.01); FPG,治疗前,与正常组相比较,模型组和治疗组及对照组均明显升高(P0.01),治疗后,与模型组相比较,对照组和治疗组均无明显降低(P0.05)；血脂,与正常组比较,模型组大鼠TG、TC、LDL-C浓度均明显升高(P0.05),HDL-C浓度明显降低(P0.05),与模型组相比较,治疗组大鼠TG、TC、LDL-C均降低(P0.05),HDL-C明显升高(P0.05),与对照组相比较,通心络高剂量治疗组大鼠血清中TG显著降低(P0.05),HDL-C显著升高(P0.05),LDL-C显著降低(P0.05); SOD活力,模型组显著低于正常组(P0.01),与模型组相比较,治疗组升高显著(P0.05),通心络高剂量组高于辛伐他汀组(P0.05)；MDA浓度,模型组显著高于正常组(P0.01),与模型组相比较,治疗组显著降低(P0.05),通心络高剂量组低于辛伐他汀组(P0.05); ROS和AGEs浓度,与模型组相比较,治疗组明显升高(P0.05)；AGEs蛋白表达,与模型组相比较,治疗组明显降低(P0.05),与辛伐他汀对照组比较,通心络高、低剂量治疗组均明显降低(P0.05)。结论：通心络可以显著改善动脉粥样硬化程度,从而有效治疗或缓解T2DM大血管病变。其作用机制可能与通心络增加SOD活力、降低ROS、AGEs和MDA浓度,下调AGEs蛋白表达等有关。提示该方可以通过调节大鼠血脂代谢情况、增强大鼠抗氧化能力及改善大鼠高糖代谢记忆效应来减缓大鼠胸主动脉病变。本研究不仅可以为益气养阴化瘀通络法治疗T2DM大血管病变提供重要的理论指导,还为今后运用中医药防治T2DM大血管病变提供了良好的基础。
[Abstract]:This paper is divided into two main parts: theoretical research and experimental research. The theoretical part systemically collated the literature of traditional Chinese medicine and Western medicine related to type 2 diabetes (T2DM) macrovascular disease, and studied the prevention and treatment of T2DM major vascular diseases by the method of nourishing qi and nourishing yin and removing blood and dredging collaterals. The T2DM rat model was established to observe the effect of Tongxinluo on T. Objective: To observe the effect of Tongxinluo on fasting blood glucose (FPG), blood lipid, superoxide dismutase (SOD), malondialdehyde (MDA), active oxygen cluster (ROS), glycosylation end product (AGEs) and the expression of AGEs protein in thoracic aorta of T2DM rats, and to explore the effect of Supplementing Qi and nourishing yin and removing stasis and dredging collaterals in the prevention and treatment of major vascular lesions in T2DM. Methods: 80 male Wistar rats were fed for 1 weeks, and then randomly divided into experimental group (70 rats) and blank group (10 rats). The experimental group was fed with high fat and high sugar diet, the blank group was fed with conventional standard feed for 4 weeks for 5 weeks, and the rats in the experimental group were intraperitoneally injected with fresh collocated 1%STZ (30mg/kg) citric acid - lime on the 5 weekend. In the blank group, the blank group was injected with the same dose of citrate sodium citrate buffer for.3 days after.3, and the fasting blood glucose was measured, and the fasting blood glucose 16.7mmol/L was considered as a successful model. The rats were divided into the model group (n=14) and the simvastatin control group (n=1). 4) Tongxinluo high dose treatment group (n=14), Tongxinluo low dose treatment group (n=14). Continue to feed high glucose and high fat diet for ninth weeks. Then the rats of Tongxinluo high, low dose group and simvastatin group were given continuous administration to twenty-first weeks.21 weekend to detect the FPG, SOD, MDA, ROS, AGEs and the thoracic aorta of AGEs protein expression level. Weight, Tongxinluo high dose treatment group was higher than model group and simvastatin control group (P0.01); FPG, before treatment, compared with normal group, model group and treatment group and control group were significantly increased (P0.01), after treatment, compared with the model group, the control group and the treatment group were not significantly decreased (P0.05); blood lipids, compared with the normal group, model group rats The concentration of TG, TC and LDL-C increased significantly (P0.05), and the concentration of HDL-C decreased significantly (P0.05). Compared with the model group, the TG, TC, LDL-C in the treatment group decreased (P0.05) and HDL-C significantly increased (P0.05). OD vitality, model group was significantly lower than normal group (P0.01), compared with model group, the treatment group increased significantly (P0.05), Tongxinluo high dose group was higher than simvastatin group (P0.05), MDA concentration, the model group was significantly higher than the normal group (P0.01), compared with the model group, treatment group significantly decreased (P0.05), Tongxinluo high dose group was lower than simvastatin group (P0.05); The concentration of ROS and AGEs was significantly higher in the treatment group than in the model group (P0.05), and the expression of AGEs protein was significantly lower in the treatment group than in the model group (P0.05). Compared with the simvastatin control group, Tongxinluo was significantly higher, and the low dose treatment group was significantly lower (P0.05). Conclusion: Tongxinluo can significantly improve the degree of atherosclerosis and thus effective. Treatment or relief of T2DM macrovascular disease, its mechanism may be associated with Tongxinluo increase SOD activity, reduce the concentration of ROS, AGEs and MDA, down the expression of AGEs protein, suggesting that the prescription can regulate the metabolism of blood lipid in rats, enhance the antioxidant capacity of rats and improve the memory effect of high glucose metabolism in rats to slow down the thoracic aorta lesion of rats. This study can not only provide important theoretical guidance for the treatment of T2DM macrovascular diseases, but also provide a good basis for the future use of traditional Chinese medicine to prevent and cure T2DM major vascular diseases.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

【相似文献】